Low-intensity ultrasound stimulation prevents osteoporotic bone loss in young adult ovariectomized mice

Dohyung Lim, Chang Yong Ko, Dong Hyun Seo, Dae Gon Woo, Jin Man Kim, Keyoung Jin Chun, Han Sung Kim

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20 Citations (Scopus)


Osteoporosis is a disease characterized by low bone mass, increased bone fragility, and a greater risk for bone fracture. Currently, pharmacological intervention can generally aid in the prevention and treatment of osteoporosis, but these therapies are often accompanied by undesirable side effects. Therefore, alternative therapies that minimize side effects are necessary. Biophysical stimuli, especially low-intensity ultrasound stimulation (LIUS), may be potential alternatives to drug-based therapies for osteoporosis. Hence, we sought to address whether LIUS therapy can effectively prevent or treat osteoporotic bone loss induced by estrogen deficiency. LIUS (1.5aMHz frequency, 1.0akHz pulse repetition on frequency, 30amW/cm2 intensity, 200aμs pulse length) was applied to right tibiae of eight 14-week-old ovariectomized virgin ICR female mice for 20amin per day, 5 days per week, over a 6-week period. Changes in 3D structural bone characteristics were detected using in vivo micro-computed tomography. Left tibiae served as controls. Structural characteristics including bone volume/tissue volume, trabecular number, trabecular bone pattern factor, and mean polar moment inertia were significantly enhanced 6 weeks after LIUS compared to the control, nonstimulated group (p<0.05). In particular, the bone volume/tissue volume in the region exposed directly to LIUS was significantly higher in the treated group (p<0.05). These findings indicate that new bone formation may be activated or that bone structure may be maintained by LIUS, and that LIUS may be effective for preventing estrogen deficiency-induced bone loss.

Original languageEnglish
Pages (from-to)116-125
Number of pages10
JournalJournal of Orthopaedic Research
Issue number1
Publication statusPublished - 2011 Jan

All Science Journal Classification (ASJC) codes

  • Orthopedics and Sports Medicine


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