Low-cost strategy for time delay adjustment of STED microscopy using digital oscilloscope

Guk Jong Choi; Won Sup Lee; Geon Lim; Hyungbae Moon; Young Pil Park; No Cheol Park

doi:10.1109/MOC.2015.7416452

Low-cost strategy for time delay adjustment of STED microscopy using digital oscilloscope

Guk Jong Choi, Won Sup Lee, Geon Lim, Hyungbae Moon, Young Pil Park, No Cheol Park

Department of Mechanical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

There has been much research about STED microscopy to reduce cost. In that microscopy, it is necessary to synchronize the pulsed lights which is just for initial setup. However, there has not been researched in order to reduce this expense until now. So, in this paper, we propose the low-cost strategy to measure the time delay in STED microscopy. To achieve that, we utilized TCSPC principle with a general digital oscilloscope. As a result, we attained successful measurement and adjustment about two pulsed lights with 180-ps delay in STED system.

Original language	English
Title of host publication	MOC 2015 - Technical Digest of 20th Microoptics Conference
Publisher	Institute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)	9784863485433
DOIs	https://doi.org/10.1109/MOC.2015.7416452
Publication status	Published - 2016 Feb 23
Event	20th Microoptics Conference, MOC 2015 - Fukuoka, Japan Duration: 2015 Oct 25 → 2015 Oct 28

Publication series

Name	MOC 2015 - Technical Digest of 20th Microoptics Conference

Other

Other	20th Microoptics Conference, MOC 2015
Country/Territory	Japan
City	Fukuoka
Period	15/10/25 → 15/10/28

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (NRF-2013R1A2A1A01016979).

Publisher Copyright:
© 2015 The Japan Society of Applied Physics.

All Science Journal Classification (ASJC) codes

Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials

Access to Document

10.1109/MOC.2015.7416452

Cite this

Choi, G. J., Lee, W. S., Lim, G., Moon, H., Park, Y. P., & Park, N. C. (2016). Low-cost strategy for time delay adjustment of STED microscopy using digital oscilloscope. In MOC 2015 - Technical Digest of 20th Microoptics Conference Article 7416452 (MOC 2015 - Technical Digest of 20th Microoptics Conference). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MOC.2015.7416452

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title = "Low-cost strategy for time delay adjustment of STED microscopy using digital oscilloscope",

abstract = "There has been much research about STED microscopy to reduce cost. In that microscopy, it is necessary to synchronize the pulsed lights which is just for initial setup. However, there has not been researched in order to reduce this expense until now. So, in this paper, we propose the low-cost strategy to measure the time delay in STED microscopy. To achieve that, we utilized TCSPC principle with a general digital oscilloscope. As a result, we attained successful measurement and adjustment about two pulsed lights with 180-ps delay in STED system.",

author = "Choi, {Guk Jong} and Lee, {Won Sup} and Geon Lim and Hyungbae Moon and Park, {Young Pil} and Park, {No Cheol}",

note = "Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (NRF-2013R1A2A1A01016979). Publisher Copyright: {\textcopyright} 2015 The Japan Society of Applied Physics.; 20th Microoptics Conference, MOC 2015 ; Conference date: 25-10-2015 Through 28-10-2015",

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doi = "10.1109/MOC.2015.7416452",

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Choi, GJ, Lee, WS, Lim, G, Moon, H, Park, YP & Park, NC 2016, Low-cost strategy for time delay adjustment of STED microscopy using digital oscilloscope. in MOC 2015 - Technical Digest of 20th Microoptics Conference., 7416452, MOC 2015 - Technical Digest of 20th Microoptics Conference, Institute of Electrical and Electronics Engineers Inc., 20th Microoptics Conference, MOC 2015, Fukuoka, Japan, 15/10/25. https://doi.org/10.1109/MOC.2015.7416452

Low-cost strategy for time delay adjustment of STED microscopy using digital oscilloscope. / Choi, Guk Jong; Lee, Won Sup; Lim, Geon et al.
MOC 2015 - Technical Digest of 20th Microoptics Conference. Institute of Electrical and Electronics Engineers Inc., 2016. 7416452 (MOC 2015 - Technical Digest of 20th Microoptics Conference).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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AU - Choi, Guk Jong

AU - Lee, Won Sup

AU - Lim, Geon

AU - Moon, Hyungbae

AU - Park, Young Pil

AU - Park, No Cheol

N1 - Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (NRF-2013R1A2A1A01016979). Publisher Copyright: © 2015 The Japan Society of Applied Physics.

PY - 2016/2/23

Y1 - 2016/2/23

N2 - There has been much research about STED microscopy to reduce cost. In that microscopy, it is necessary to synchronize the pulsed lights which is just for initial setup. However, there has not been researched in order to reduce this expense until now. So, in this paper, we propose the low-cost strategy to measure the time delay in STED microscopy. To achieve that, we utilized TCSPC principle with a general digital oscilloscope. As a result, we attained successful measurement and adjustment about two pulsed lights with 180-ps delay in STED system.

AB - There has been much research about STED microscopy to reduce cost. In that microscopy, it is necessary to synchronize the pulsed lights which is just for initial setup. However, there has not been researched in order to reduce this expense until now. So, in this paper, we propose the low-cost strategy to measure the time delay in STED microscopy. To achieve that, we utilized TCSPC principle with a general digital oscilloscope. As a result, we attained successful measurement and adjustment about two pulsed lights with 180-ps delay in STED system.

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Low-cost strategy for time delay adjustment of STED microscopy using digital oscilloscope

Abstract

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Bibliographical note

All Science Journal Classification (ASJC) codes

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