Lipopolysaccharide temporarily impairs sphincter of Oddi motility

Joseph J. Cullen, Hyojin Park, Kimberly S. Ephgrave, Jeffrey L. Conklin

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


The aim of our study was to determine the effect of lipopolysaccharide (LPS) on sphincter of Oddi (SO) motility. Opossums received saline, Escherichia coli LPS (1.0 mg/kg), or E. coli LPS (1.0 mg/kg) and aminoguanidine (50 mg/kg), and the SO was removed 6-24 h later. At 12 h LPS decreased electrical field stimulation (EFS)-induced relaxation and increased baseline tone. These changes were reversed when the animals were pretreated with aminoguanidine. The dose-dependent decrease in EFS-induced relaxation by Nω-nitro-L-arginine was impaired after LPS, but not in animals that received LPS and aminoguanidine. The impaired EFS-induced relaxation after LPS was reversed when L-arginine was added to the tissue bath. Serum levels of NO2-/NO3- were increased with LPS as compared to saline or both LPS and aminoguanidine. Inducible nitric oxide synthase mRNA was readily seen in SO segments after LPS. LPS impairs EFS-induced relaxation and increases baseline tone of the SO. The effects of LPS on SO motility appear to be mediated by nitric oxide.

Original languageEnglish
Pages (from-to)547-554
Number of pages8
JournalNitric Oxide - Biology and Chemistry
Issue number6
Publication statusPublished - 2001

Bibliographical note

Funding Information:
1 A portion of this manuscript was presented at annual meeting of the American Gastroenterological Association, May 16–19, 1999, Orlando, Florida, and published in abstract form in Gastroenterology 116(A7). Supported by Merit Review awards (J.J.C. and J.L.C.) from the Department of Veterans Affairs.

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Clinical Biochemistry
  • Cancer Research


Dive into the research topics of 'Lipopolysaccharide temporarily impairs sphincter of Oddi motility'. Together they form a unique fingerprint.

Cite this