Light-sheet microscopy using an Airy beam

Tom Vettenburg; Heather I.C. Dalgarno; Jonathan Nylk; Clara Coll-Lladó; David E.K. Ferrier; Tomáš Čižmár; Frank J. Gunn-Moore; Kishan Dholakia

doi:10.1038/nmeth.2922

Light-sheet microscopy using an Airy beam

Tom Vettenburg, Heather I.C. Dalgarno, Jonathan Nylk, Clara Coll-Lladó, David E.K. Ferrier, Tomáš Čižmár, Frank J. Gunn-Moore, Kishan Dholakia

Department of Physics

Research output: Contribution to journal › Article › peer-review

491 Citations (Scopus)

Abstract

Light-sheet microscopy facilitates rapid, high-contrast, volumetric imaging with minimal sample exposure. However, the rapid divergence of a traditional Gaussian light sheet restricts the field of view (FOV) that provides innate subcellular resolution. We show that the Airy beam innately yields high contrast and resolution up to a tenfold larger FOV. In contrast to the Bessel beam, which also provides an increased FOV, the Airy beam's characteristic asymmetric excitation pattern results in all fluorescence contributing positively to the contrast, enabling a step change for light-sheet microscopy.

Original language	English
Pages (from-to)	541-544
Number of pages	4
Journal	Nature Methods
Volume	11
Issue number	5
DOIs	https://doi.org/10.1038/nmeth.2922
Publication status	Published - 2014 May

Bibliographical note

Funding Information:
The project was supported by the UK Engineering and Physical Sciences Research Council, RS Macdonald Charitable Trust, Scottish Universities Life Sciences Alliance and St Andrews 600 anniversary BRAINS appeal. The PhD apprenticeship of C.C.L. was funded by the School of Biology. The authors thank A.M. Tilston-Lunel, M.L. Torres-Mapa, Z. Allen and A. Chatterton for their assistance with the cell culture; M. Ploschner for advice and proofreading of the manuscript; P.A. Reynolds for the fruitful discussions on the biological aspects of this study; and C. Penedo for his assistance with the photobleaching studies.

All Science Journal Classification (ASJC) codes

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth.2922

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title = "Light-sheet microscopy using an Airy beam",

abstract = "Light-sheet microscopy facilitates rapid, high-contrast, volumetric imaging with minimal sample exposure. However, the rapid divergence of a traditional Gaussian light sheet restricts the field of view (FOV) that provides innate subcellular resolution. We show that the Airy beam innately yields high contrast and resolution up to a tenfold larger FOV. In contrast to the Bessel beam, which also provides an increased FOV, the Airy beam's characteristic asymmetric excitation pattern results in all fluorescence contributing positively to the contrast, enabling a step change for light-sheet microscopy.",

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note = "Funding Information: The project was supported by the UK Engineering and Physical Sciences Research Council, RS Macdonald Charitable Trust, Scottish Universities Life Sciences Alliance and St Andrews 600 anniversary BRAINS appeal. The PhD apprenticeship of C.C.L. was funded by the School of Biology. The authors thank A.M. Tilston-Lunel, M.L. Torres-Mapa, Z. Allen and A. Chatterton for their assistance with the cell culture; M. Ploschner for advice and proofreading of the manuscript; P.A. Reynolds for the fruitful discussions on the biological aspects of this study; and C. Penedo for his assistance with the photobleaching studies.",

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doi = "10.1038/nmeth.2922",

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AU - Vettenburg, Tom

AU - Dalgarno, Heather I.C.

AU - Nylk, Jonathan

AU - Coll-Lladó, Clara

AU - Ferrier, David E.K.

AU - Čižmár, Tomáš

AU - Gunn-Moore, Frank J.

AU - Dholakia, Kishan

N1 - Funding Information: The project was supported by the UK Engineering and Physical Sciences Research Council, RS Macdonald Charitable Trust, Scottish Universities Life Sciences Alliance and St Andrews 600 anniversary BRAINS appeal. The PhD apprenticeship of C.C.L. was funded by the School of Biology. The authors thank A.M. Tilston-Lunel, M.L. Torres-Mapa, Z. Allen and A. Chatterton for their assistance with the cell culture; M. Ploschner for advice and proofreading of the manuscript; P.A. Reynolds for the fruitful discussions on the biological aspects of this study; and C. Penedo for his assistance with the photobleaching studies.

PY - 2014/5

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AB - Light-sheet microscopy facilitates rapid, high-contrast, volumetric imaging with minimal sample exposure. However, the rapid divergence of a traditional Gaussian light sheet restricts the field of view (FOV) that provides innate subcellular resolution. We show that the Airy beam innately yields high contrast and resolution up to a tenfold larger FOV. In contrast to the Bessel beam, which also provides an increased FOV, the Airy beam's characteristic asymmetric excitation pattern results in all fluorescence contributing positively to the contrast, enabling a step change for light-sheet microscopy.

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Light-sheet microscopy using an Airy beam

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Bibliographical note

All Science Journal Classification (ASJC) codes

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