Light-sheet fluorescence microscopy with structured light

Jonathan Nylk; Kishan Dholakia

doi:10.1016/B978-0-323-48067-3.00018-4

Light-sheet fluorescence microscopy with structured light

Jonathan Nylk, Kishan Dholakia

Department of Physics

Research output: Chapter in Book/Report/Conference proceeding › Chapter

2 Citations (Scopus)

Abstract

Light-sheet microscopy has emerged as a powerful approach for long-term imaging within neuroscience and developmental biology. This chapter will cover the basics of light-sheet microscopy with an emphasis on structured light fields, namely propagation-invariant beams. The use of such fields allows the field-of-view to be maximized while retaining high resolution. There are additional advantages of minimizing photodamage.

Original language	English
Title of host publication	Neurophotonics and Biomedical Spectroscopy
Publisher	Elsevier
Pages	477-501
Number of pages	25
ISBN (Electronic)	9780323480673
ISBN (Print)	9780323480680
DOIs	https://doi.org/10.1016/B978-0-323-48067-3.00018-4
Publication status	Published - 2018 Jan 1

Bibliographical note

Publisher Copyright:
© 2019 Elsevier Ltd. All rights reserved.

All Science Journal Classification (ASJC) codes

General Engineering
General Materials Science

Access to Document

10.1016/B978-0-323-48067-3.00018-4

Cite this

@inbook{262195cca3cc489b95c24a6a5fcfdb66,

title = "Light-sheet fluorescence microscopy with structured light",

abstract = "Light-sheet microscopy has emerged as a powerful approach for long-term imaging within neuroscience and developmental biology. This chapter will cover the basics of light-sheet microscopy with an emphasis on structured light fields, namely propagation-invariant beams. The use of such fields allows the field-of-view to be maximized while retaining high resolution. There are additional advantages of minimizing photodamage.",

keywords = "Airy beam, Beam shaping, Bessel beam, Developmental biology, Fluorescence, Holography, Light-sheet microscopy, Microscopy, Neuroscience, Optical sectioning, Structured light",

author = "Jonathan Nylk and Kishan Dholakia",

year = "2018",

month = jan,

day = "1",

doi = "10.1016/B978-0-323-48067-3.00018-4",

language = "English",

isbn = "9780323480680",

pages = "477--501",

booktitle = "Neurophotonics and Biomedical Spectroscopy",

publisher = "Elsevier",

address = "Netherlands",

}

TY - CHAP

T1 - Light-sheet fluorescence microscopy with structured light

AU - Nylk, Jonathan

AU - Dholakia, Kishan

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Light-sheet microscopy has emerged as a powerful approach for long-term imaging within neuroscience and developmental biology. This chapter will cover the basics of light-sheet microscopy with an emphasis on structured light fields, namely propagation-invariant beams. The use of such fields allows the field-of-view to be maximized while retaining high resolution. There are additional advantages of minimizing photodamage.

AB - Light-sheet microscopy has emerged as a powerful approach for long-term imaging within neuroscience and developmental biology. This chapter will cover the basics of light-sheet microscopy with an emphasis on structured light fields, namely propagation-invariant beams. The use of such fields allows the field-of-view to be maximized while retaining high resolution. There are additional advantages of minimizing photodamage.

KW - Airy beam

KW - Beam shaping

KW - Bessel beam

KW - Developmental biology

KW - Fluorescence

KW - Holography

KW - Light-sheet microscopy

KW - Microscopy

KW - Neuroscience

KW - Optical sectioning

KW - Structured light

UR - http://www.scopus.com/inward/record.url?scp=85064805651&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85064805651&partnerID=8YFLogxK

U2 - 10.1016/B978-0-323-48067-3.00018-4

DO - 10.1016/B978-0-323-48067-3.00018-4

M3 - Chapter

AN - SCOPUS:85064805651

SN - 9780323480680

SP - 477

EP - 501

BT - Neurophotonics and Biomedical Spectroscopy

PB - Elsevier

ER -

Light-sheet fluorescence microscopy with structured light

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this