Light-sheet fluorescence microscopy with structured light

Jonathan Nylk, Kishan Dholakia

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)


Light-sheet microscopy has emerged as a powerful approach for long-term imaging within neuroscience and developmental biology. This chapter will cover the basics of light-sheet microscopy with an emphasis on structured light fields, namely propagation-invariant beams. The use of such fields allows the field-of-view to be maximized while retaining high resolution. There are additional advantages of minimizing photodamage.

Original languageEnglish
Title of host publicationNeurophotonics and Biomedical Spectroscopy
Number of pages25
ISBN (Electronic)9780323480673
ISBN (Print)9780323480680
Publication statusPublished - 2018 Jan 1

Bibliographical note

Publisher Copyright:
© 2019 Elsevier Ltd. All rights reserved.

All Science Journal Classification (ASJC) codes

  • Engineering(all)
  • Materials Science(all)


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