Large-scale analysis of the GRAS gene family in Arabidopsis thaliana

Mi Hyun Lee; Bohye Kim; Sang Kee Song; Jung Ok Heo; Nan Ie Yu; Shin Ae Lee; Miran Kim; Dong Gwan Kim; Sung Oh Sohn; Chae Eun Lim; Kwang Suk Chang; Myeong Min Lee; Jun Lim

doi:10.1007/s11103-008-9345-1

Large-scale analysis of the GRAS gene family in Arabidopsis thaliana

Mi Hyun Lee, Bohye Kim, Sang Kee Song, Jung Ok Heo, Nan Ie Yu, Shin Ae Lee, Miran Kim, Dong Gwan Kim, Sung Oh Sohn, Chae Eun Lim, Kwang Suk Chang, Myeong Min Lee, Jun Lim

Research output: Contribution to journal › Article › peer-review

159 Citations (Scopus)

Abstract

GRAS proteins belong to a plant-specific transcription factor family. Currently, 33 GRAS members including a putative expressed pseudogene have been identified in the Arabidopsis genome. With a reverse genetic approach, we have constructed a "phenome-ready unimutant collection" of the GRAS genes in Arabidopsis thaliana. Of this collection, we focused on loss-of-function mutations in 23 novel GRAS members. Under standard conditions, homozygous mutants have no obvious morphological phenotypes compared with those of wild-type plants. Expression analysis of GRAS genes using quantitative real-time RT-PCR (qRT-PCR), microarray data mining, and promoter::GUS reporter fusions revealed their tissue-specific expression patterns. Our analysis of protein-protein interaction and subcellular localization of individual GRAS members indicated their roles as transcription regulators. In our yeast two-hybrid (Y2H) assay, we confirmed the protein-protein interaction between SHORT-ROOT (SHR) and SCARECROW (SCR). Furthermore, we identified a new SHR-interacting protein, SCARECROW-LIKE23 (SCL23), which is the most closely related to SCR. Our large-scale analysis provides a comprehensive evaluation on the Arabidopsis GRAS members, and also our phenome-ready unimutant collection will be a useful resource to better understand individual GRAS proteins that play diverse roles in plant growth and development.

Original language	English
Pages (from-to)	659-670
Number of pages	12
Journal	Plant Molecular Biology
Volume	67
Issue number	6
DOIs	https://doi.org/10.1007/s11103-008-9345-1
Publication status	Published - 2008 Aug

Bibliographical note

Funding Information:
Acknowledgments The authors wish to thank ABRC, NASC, and RIKEN for providing the seeds and cDNA clones. We also thank Yrjo Helariutta for critical reading of the manuscript. We are also grateful to In A Kim and Ji Hye Yoon for technical assistance and plant maintenance. This work was supported by grants from Plant Signaling Network Research Center (R11-2003-008-03004-0) and KRF (C00469) to MML; KOSEF (R01-2006-000-11026-0), KRF (C00131), Crop Functional Genomics Center (CG1123), and Bio-Green 21 to JL.

All Science Journal Classification (ASJC) codes

Genetics
Agronomy and Crop Science
Plant Science

Access to Document

10.1007/s11103-008-9345-1

Cite this

@article{842483a44b2b4e93ac7241f3595bda8d,

title = "Large-scale analysis of the GRAS gene family in Arabidopsis thaliana",

abstract = "GRAS proteins belong to a plant-specific transcription factor family. Currently, 33 GRAS members including a putative expressed pseudogene have been identified in the Arabidopsis genome. With a reverse genetic approach, we have constructed a {"}phenome-ready unimutant collection{"} of the GRAS genes in Arabidopsis thaliana. Of this collection, we focused on loss-of-function mutations in 23 novel GRAS members. Under standard conditions, homozygous mutants have no obvious morphological phenotypes compared with those of wild-type plants. Expression analysis of GRAS genes using quantitative real-time RT-PCR (qRT-PCR), microarray data mining, and promoter::GUS reporter fusions revealed their tissue-specific expression patterns. Our analysis of protein-protein interaction and subcellular localization of individual GRAS members indicated their roles as transcription regulators. In our yeast two-hybrid (Y2H) assay, we confirmed the protein-protein interaction between SHORT-ROOT (SHR) and SCARECROW (SCR). Furthermore, we identified a new SHR-interacting protein, SCARECROW-LIKE23 (SCL23), which is the most closely related to SCR. Our large-scale analysis provides a comprehensive evaluation on the Arabidopsis GRAS members, and also our phenome-ready unimutant collection will be a useful resource to better understand individual GRAS proteins that play diverse roles in plant growth and development.",

author = "Lee, {Mi Hyun} and Bohye Kim and Song, {Sang Kee} and Heo, {Jung Ok} and Yu, {Nan Ie} and Lee, {Shin Ae} and Miran Kim and Kim, {Dong Gwan} and Sohn, {Sung Oh} and Lim, {Chae Eun} and Chang, {Kwang Suk} and Lee, {Myeong Min} and Jun Lim",

note = "Funding Information: Acknowledgments The authors wish to thank ABRC, NASC, and RIKEN for providing the seeds and cDNA clones. We also thank Yrjo Helariutta for critical reading of the manuscript. We are also grateful to In A Kim and Ji Hye Yoon for technical assistance and plant maintenance. This work was supported by grants from Plant Signaling Network Research Center (R11-2003-008-03004-0) and KRF (C00469) to MML; KOSEF (R01-2006-000-11026-0), KRF (C00131), Crop Functional Genomics Center (CG1123), and Bio-Green 21 to JL.",

year = "2008",

month = aug,

doi = "10.1007/s11103-008-9345-1",

language = "English",

volume = "67",

pages = "659--670",

journal = "Plant Molecular Biology",

issn = "0167-4412",

publisher = "Springer Netherlands",

number = "6",

}

TY - JOUR

T1 - Large-scale analysis of the GRAS gene family in Arabidopsis thaliana

AU - Lee, Mi Hyun

AU - Kim, Bohye

AU - Song, Sang Kee

AU - Heo, Jung Ok

AU - Yu, Nan Ie

AU - Lee, Shin Ae

AU - Kim, Miran

AU - Kim, Dong Gwan

AU - Sohn, Sung Oh

AU - Lim, Chae Eun

AU - Chang, Kwang Suk

AU - Lee, Myeong Min

AU - Lim, Jun

N1 - Funding Information: Acknowledgments The authors wish to thank ABRC, NASC, and RIKEN for providing the seeds and cDNA clones. We also thank Yrjo Helariutta for critical reading of the manuscript. We are also grateful to In A Kim and Ji Hye Yoon for technical assistance and plant maintenance. This work was supported by grants from Plant Signaling Network Research Center (R11-2003-008-03004-0) and KRF (C00469) to MML; KOSEF (R01-2006-000-11026-0), KRF (C00131), Crop Functional Genomics Center (CG1123), and Bio-Green 21 to JL.

PY - 2008/8

Y1 - 2008/8

N2 - GRAS proteins belong to a plant-specific transcription factor family. Currently, 33 GRAS members including a putative expressed pseudogene have been identified in the Arabidopsis genome. With a reverse genetic approach, we have constructed a "phenome-ready unimutant collection" of the GRAS genes in Arabidopsis thaliana. Of this collection, we focused on loss-of-function mutations in 23 novel GRAS members. Under standard conditions, homozygous mutants have no obvious morphological phenotypes compared with those of wild-type plants. Expression analysis of GRAS genes using quantitative real-time RT-PCR (qRT-PCR), microarray data mining, and promoter::GUS reporter fusions revealed their tissue-specific expression patterns. Our analysis of protein-protein interaction and subcellular localization of individual GRAS members indicated their roles as transcription regulators. In our yeast two-hybrid (Y2H) assay, we confirmed the protein-protein interaction between SHORT-ROOT (SHR) and SCARECROW (SCR). Furthermore, we identified a new SHR-interacting protein, SCARECROW-LIKE23 (SCL23), which is the most closely related to SCR. Our large-scale analysis provides a comprehensive evaluation on the Arabidopsis GRAS members, and also our phenome-ready unimutant collection will be a useful resource to better understand individual GRAS proteins that play diverse roles in plant growth and development.

AB - GRAS proteins belong to a plant-specific transcription factor family. Currently, 33 GRAS members including a putative expressed pseudogene have been identified in the Arabidopsis genome. With a reverse genetic approach, we have constructed a "phenome-ready unimutant collection" of the GRAS genes in Arabidopsis thaliana. Of this collection, we focused on loss-of-function mutations in 23 novel GRAS members. Under standard conditions, homozygous mutants have no obvious morphological phenotypes compared with those of wild-type plants. Expression analysis of GRAS genes using quantitative real-time RT-PCR (qRT-PCR), microarray data mining, and promoter::GUS reporter fusions revealed their tissue-specific expression patterns. Our analysis of protein-protein interaction and subcellular localization of individual GRAS members indicated their roles as transcription regulators. In our yeast two-hybrid (Y2H) assay, we confirmed the protein-protein interaction between SHORT-ROOT (SHR) and SCARECROW (SCR). Furthermore, we identified a new SHR-interacting protein, SCARECROW-LIKE23 (SCL23), which is the most closely related to SCR. Our large-scale analysis provides a comprehensive evaluation on the Arabidopsis GRAS members, and also our phenome-ready unimutant collection will be a useful resource to better understand individual GRAS proteins that play diverse roles in plant growth and development.

UR - http://www.scopus.com/inward/record.url?scp=46249113503&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=46249113503&partnerID=8YFLogxK

U2 - 10.1007/s11103-008-9345-1

DO - 10.1007/s11103-008-9345-1

M3 - Article

C2 - 18500650

AN - SCOPUS:46249113503

SN - 0167-4412

VL - 67

SP - 659

EP - 670

JO - Plant Molecular Biology

JF - Plant Molecular Biology

IS - 6

ER -

Large-scale analysis of the GRAS gene family in Arabidopsis thaliana

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this