Lactobacillus plantarum CBT LP3 ameliorates colitis via modulating T cells in mice

Da Hye Kim; Soochan Kim; Jae Bum Ahn; Jae Hyeon Kim; Hyun Woo Ma; Dong Hyuk Seo; Xiumei Che; Ki Cheong Park; Jeong Yong Jeon; Sang Yong Kim; Han Cheol Lee; Jae Young Lee; Tae Il Kim; Won Ho Kim; Seung Won Kim; Jae Hee Cheon

doi:10.1016/j.ijmm.2020.151391

Lactobacillus plantarum CBT LP3 ameliorates colitis via modulating T cells in mice

Da Hye Kim, Soochan Kim, Jae Bum Ahn, Jae Hyeon Kim, Hyun Woo Ma, Dong Hyuk Seo, Xiumei Che, Ki Cheong Park, Jeong Yong Jeon, Sang Yong Kim, Han Cheol Lee, Jae Young Lee, Tae Il Kim, Won Ho Kim, Seung Won Kim, Jae Hee Cheon

Department of Internal Medicine

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Lactobacillus plantarum has been identified as a probiotic bacterium owing to its role in immune regulation and maintenance of intestinal permeability. Here, we investigated the anti-colitic effects and mechanism of L. plantarum CBT LP3 (LP3). This in vivo study was performed using dextran sodium sulfate (DSS) to induce colitis in mice. Mice were randomly divided into three groups: a control supplied with normal drinking water, a DSS-treated group followed by oral administration of vehicle, and a DSS-treated group gavaged with LP3 daily for 7 days following DSS administration. An analysis of macrophages and T cell subsets harvesting from peritonium cavity cells and splenocytes was performed using a flow cytometric assay. Gene expression and cytokine profiles were measured using quantitative reverse transcriptase polymerase chain reaction. The administration of LP3 significantly attenuated disease activity and histolopathology compared to control. LP3 had anti-inflammatory effects, with increased induction of regulatory T cells and type 2 helper T cells in splenocytes and restoration of goblet cells accompanied by suppression of proinflammatory cytokine expressions. These findings suggest that L. plantarum CBT LP3 can be used as a potent immunomodulator, which has significant implications for IBD treatment.

Original language	English
Article number	151391
Journal	International Journal of Medical Microbiology
Volume	310
Issue number	2
DOIs	https://doi.org/10.1016/j.ijmm.2020.151391
Publication status	Published - 2020 Feb

Bibliographical note

Funding Information:
This work was supported by a grant from the Ministry of Health & Welfare, Republic of Korea (grant number: HI14C1324), a Mid-career Researcher Program through an NRF grant funded by the Korean government (MSIP) (NRF-2017R1A2B4001848), and Health Fellowship Foundation, as well as Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Agricultural Microbiome R&D Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (918003041SB010).

Funding Information:
This work was supported by a grant from the Ministry of Health & Welfare, Republic of Korea (grant number: HI14C1324 ), a Mid-career Researcher Program through an NRF grant funded by the Korean government ( MSIP ) (NRF- 2017R1A2B4001848 ), and Health Fellowship Foundation, as well as Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Agricultural Microbiome R&D Program, funded by Ministry of Agriculture, Food and Rural Affairs ( MAFRA ) ( 918003041SB010 ).

Publisher Copyright:
© 2020 The Authors

All Science Journal Classification (ASJC) codes

Microbiology
Microbiology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.ijmm.2020.151391

Cite this

Kim, D. H., Kim, S., Ahn, J. B., Kim, J. H., Ma, H. W., Seo, D. H., Che, X., Park, K. C., Jeon, J. Y., Kim, S. Y., Lee, H. C., Lee, J. Y., Kim, T. I., Kim, W. H., Kim, S. W., & Cheon, J. H. (2020). Lactobacillus plantarum CBT LP3 ameliorates colitis via modulating T cells in mice. International Journal of Medical Microbiology, 310(2), [151391]. https://doi.org/10.1016/j.ijmm.2020.151391

@article{a3c815ec77b24e3eb67571a55f37e403,

title = "Lactobacillus plantarum CBT LP3 ameliorates colitis via modulating T cells in mice",

abstract = "Lactobacillus plantarum has been identified as a probiotic bacterium owing to its role in immune regulation and maintenance of intestinal permeability. Here, we investigated the anti-colitic effects and mechanism of L. plantarum CBT LP3 (LP3). This in vivo study was performed using dextran sodium sulfate (DSS) to induce colitis in mice. Mice were randomly divided into three groups: a control supplied with normal drinking water, a DSS-treated group followed by oral administration of vehicle, and a DSS-treated group gavaged with LP3 daily for 7 days following DSS administration. An analysis of macrophages and T cell subsets harvesting from peritonium cavity cells and splenocytes was performed using a flow cytometric assay. Gene expression and cytokine profiles were measured using quantitative reverse transcriptase polymerase chain reaction. The administration of LP3 significantly attenuated disease activity and histolopathology compared to control. LP3 had anti-inflammatory effects, with increased induction of regulatory T cells and type 2 helper T cells in splenocytes and restoration of goblet cells accompanied by suppression of proinflammatory cytokine expressions. These findings suggest that L. plantarum CBT LP3 can be used as a potent immunomodulator, which has significant implications for IBD treatment.",

author = "Kim, {Da Hye} and Soochan Kim and Ahn, {Jae Bum} and Kim, {Jae Hyeon} and Ma, {Hyun Woo} and Seo, {Dong Hyuk} and Xiumei Che and Park, {Ki Cheong} and Jeon, {Jeong Yong} and Kim, {Sang Yong} and Lee, {Han Cheol} and Lee, {Jae Young} and Kim, {Tae Il} and Kim, {Won Ho} and Kim, {Seung Won} and Cheon, {Jae Hee}",

note = "Funding Information: This work was supported by a grant from the Ministry of Health & Welfare, Republic of Korea (grant number: HI14C1324), a Mid-career Researcher Program through an NRF grant funded by the Korean government (MSIP) (NRF-2017R1A2B4001848), and Health Fellowship Foundation, as well as Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Agricultural Microbiome R&D Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (918003041SB010). Funding Information: This work was supported by a grant from the Ministry of Health & Welfare, Republic of Korea (grant number: HI14C1324 ), a Mid-career Researcher Program through an NRF grant funded by the Korean government ( MSIP ) (NRF- 2017R1A2B4001848 ), and Health Fellowship Foundation, as well as Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Agricultural Microbiome R&D Program, funded by Ministry of Agriculture, Food and Rural Affairs ( MAFRA ) ( 918003041SB010 ). Publisher Copyright: {\textcopyright} 2020 The Authors",

year = "2020",

month = feb,

doi = "10.1016/j.ijmm.2020.151391",

language = "English",

volume = "310",

journal = "International Journal of Medical Microbiology",

issn = "1438-4221",

publisher = "Urban und Fischer Verlag GmbH und Co. KG",

number = "2",

}

TY - JOUR

T1 - Lactobacillus plantarum CBT LP3 ameliorates colitis via modulating T cells in mice

AU - Kim, Da Hye

AU - Kim, Soochan

AU - Ahn, Jae Bum

AU - Kim, Jae Hyeon

AU - Ma, Hyun Woo

AU - Seo, Dong Hyuk

AU - Che, Xiumei

AU - Park, Ki Cheong

AU - Jeon, Jeong Yong

AU - Kim, Sang Yong

AU - Lee, Han Cheol

AU - Lee, Jae Young

AU - Kim, Tae Il

AU - Kim, Won Ho

AU - Kim, Seung Won

AU - Cheon, Jae Hee

N1 - Funding Information: This work was supported by a grant from the Ministry of Health & Welfare, Republic of Korea (grant number: HI14C1324), a Mid-career Researcher Program through an NRF grant funded by the Korean government (MSIP) (NRF-2017R1A2B4001848), and Health Fellowship Foundation, as well as Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Agricultural Microbiome R&D Program, funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (918003041SB010). Funding Information: This work was supported by a grant from the Ministry of Health & Welfare, Republic of Korea (grant number: HI14C1324 ), a Mid-career Researcher Program through an NRF grant funded by the Korean government ( MSIP ) (NRF- 2017R1A2B4001848 ), and Health Fellowship Foundation, as well as Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Agricultural Microbiome R&D Program, funded by Ministry of Agriculture, Food and Rural Affairs ( MAFRA ) ( 918003041SB010 ). Publisher Copyright: © 2020 The Authors

PY - 2020/2

Y1 - 2020/2

N2 - Lactobacillus plantarum has been identified as a probiotic bacterium owing to its role in immune regulation and maintenance of intestinal permeability. Here, we investigated the anti-colitic effects and mechanism of L. plantarum CBT LP3 (LP3). This in vivo study was performed using dextran sodium sulfate (DSS) to induce colitis in mice. Mice were randomly divided into three groups: a control supplied with normal drinking water, a DSS-treated group followed by oral administration of vehicle, and a DSS-treated group gavaged with LP3 daily for 7 days following DSS administration. An analysis of macrophages and T cell subsets harvesting from peritonium cavity cells and splenocytes was performed using a flow cytometric assay. Gene expression and cytokine profiles were measured using quantitative reverse transcriptase polymerase chain reaction. The administration of LP3 significantly attenuated disease activity and histolopathology compared to control. LP3 had anti-inflammatory effects, with increased induction of regulatory T cells and type 2 helper T cells in splenocytes and restoration of goblet cells accompanied by suppression of proinflammatory cytokine expressions. These findings suggest that L. plantarum CBT LP3 can be used as a potent immunomodulator, which has significant implications for IBD treatment.

AB - Lactobacillus plantarum has been identified as a probiotic bacterium owing to its role in immune regulation and maintenance of intestinal permeability. Here, we investigated the anti-colitic effects and mechanism of L. plantarum CBT LP3 (LP3). This in vivo study was performed using dextran sodium sulfate (DSS) to induce colitis in mice. Mice were randomly divided into three groups: a control supplied with normal drinking water, a DSS-treated group followed by oral administration of vehicle, and a DSS-treated group gavaged with LP3 daily for 7 days following DSS administration. An analysis of macrophages and T cell subsets harvesting from peritonium cavity cells and splenocytes was performed using a flow cytometric assay. Gene expression and cytokine profiles were measured using quantitative reverse transcriptase polymerase chain reaction. The administration of LP3 significantly attenuated disease activity and histolopathology compared to control. LP3 had anti-inflammatory effects, with increased induction of regulatory T cells and type 2 helper T cells in splenocytes and restoration of goblet cells accompanied by suppression of proinflammatory cytokine expressions. These findings suggest that L. plantarum CBT LP3 can be used as a potent immunomodulator, which has significant implications for IBD treatment.

UR - http://www.scopus.com/inward/record.url?scp=85078743028&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85078743028&partnerID=8YFLogxK

U2 - 10.1016/j.ijmm.2020.151391

DO - 10.1016/j.ijmm.2020.151391

M3 - Article

C2 - 32007342

AN - SCOPUS:85078743028

SN - 1438-4221

VL - 310

JO - International Journal of Medical Microbiology

JF - International Journal of Medical Microbiology

IS - 2

M1 - 151391

ER -

Lactobacillus plantarum CBT LP3 ameliorates colitis via modulating T cells in mice

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this