Label-free protein assay with site-directly immobilized antibody using self-actuating PZT cantilever

Ghi Yuun Kang; Ga Young Han; Ji Yoon Kang; Il Hoon Cho; Hyung Ho Park; Se Hwan Paek; Tae Song Kim

doi:10.1016/j.snb.2005.11.011

Label-free protein assay with site-directly immobilized antibody using self-actuating PZT cantilever

Ghi Yuun Kang, Ga Young Han, Ji Yoon Kang, Il Hoon Cho, Hyung Ho Park, Se Hwan Paek, Tae Song Kim

Department of Materials Science and Engineering

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

This paper reports a method to improve the limit of the label-free detection of myoglobin using site-directed immobilization on the surface of self-actuating PZT cantilever sensor. The resonant frequency change of cantilever was measured electrically using piezoelectricity when antibody-antigen interaction occurred. Site-directed antibody was biotinylated after reducing the binding region to hinge region of randomly biotinylated antibody, which increased the number of effective binding sites. Investigating the effect of site-directed antibody on the cantilever sensor revealed that this protocol was able to enhance the performance of analytical sensitivity about 10 times with the modification of sensing layer. The experimental results demonstrated this biosensor can be used for diagnosis of acute myocardial infarction in clinical range of tens of ng/ml.

Original language	English
Pages (from-to)	332-338
Number of pages	7
Journal	Sensors and Actuators, B: Chemical
Volume	117
Issue number	2
DOIs	https://doi.org/10.1016/j.snb.2005.11.011
Publication status	Published - 2006 Oct 12

Bibliographical note

Funding Information:
This research has been supported by the Intelligent Microsystem Center (IMC; http://www.microsystem.re.kr ), which carries out one of the 21st century's Frontier R&D Projects sponsored by the Korea Ministry Of Commerce, Industry and Energy and a KIST GRANT Program sponsored by KIST.

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

Access to Document

10.1016/j.snb.2005.11.011

Cite this

@article{d7a8464a46ab435d8405ea5cba677e20,

title = "Label-free protein assay with site-directly immobilized antibody using self-actuating PZT cantilever",

abstract = "This paper reports a method to improve the limit of the label-free detection of myoglobin using site-directed immobilization on the surface of self-actuating PZT cantilever sensor. The resonant frequency change of cantilever was measured electrically using piezoelectricity when antibody-antigen interaction occurred. Site-directed antibody was biotinylated after reducing the binding region to hinge region of randomly biotinylated antibody, which increased the number of effective binding sites. Investigating the effect of site-directed antibody on the cantilever sensor revealed that this protocol was able to enhance the performance of analytical sensitivity about 10 times with the modification of sensing layer. The experimental results demonstrated this biosensor can be used for diagnosis of acute myocardial infarction in clinical range of tens of ng/ml.",

author = "Kang, {Ghi Yuun} and Han, {Ga Young} and Kang, {Ji Yoon} and Cho, {Il Hoon} and Park, {Hyung Ho} and Paek, {Se Hwan} and Kim, {Tae Song}",

note = "Funding Information: This research has been supported by the Intelligent Microsystem Center (IMC; http://www.microsystem.re.kr ), which carries out one of the 21st century's Frontier R&D Projects sponsored by the Korea Ministry Of Commerce, Industry and Energy and a KIST GRANT Program sponsored by KIST. ",

year = "2006",

month = oct,

day = "12",

doi = "10.1016/j.snb.2005.11.011",

language = "English",

volume = "117",

pages = "332--338",

journal = "Sensors and Actuators, B: Chemical",

issn = "0925-4005",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Label-free protein assay with site-directly immobilized antibody using self-actuating PZT cantilever

AU - Kang, Ghi Yuun

AU - Han, Ga Young

AU - Kang, Ji Yoon

AU - Cho, Il Hoon

AU - Park, Hyung Ho

AU - Paek, Se Hwan

AU - Kim, Tae Song

N1 - Funding Information: This research has been supported by the Intelligent Microsystem Center (IMC; http://www.microsystem.re.kr ), which carries out one of the 21st century's Frontier R&D Projects sponsored by the Korea Ministry Of Commerce, Industry and Energy and a KIST GRANT Program sponsored by KIST.

PY - 2006/10/12

Y1 - 2006/10/12

N2 - This paper reports a method to improve the limit of the label-free detection of myoglobin using site-directed immobilization on the surface of self-actuating PZT cantilever sensor. The resonant frequency change of cantilever was measured electrically using piezoelectricity when antibody-antigen interaction occurred. Site-directed antibody was biotinylated after reducing the binding region to hinge region of randomly biotinylated antibody, which increased the number of effective binding sites. Investigating the effect of site-directed antibody on the cantilever sensor revealed that this protocol was able to enhance the performance of analytical sensitivity about 10 times with the modification of sensing layer. The experimental results demonstrated this biosensor can be used for diagnosis of acute myocardial infarction in clinical range of tens of ng/ml.

AB - This paper reports a method to improve the limit of the label-free detection of myoglobin using site-directed immobilization on the surface of self-actuating PZT cantilever sensor. The resonant frequency change of cantilever was measured electrically using piezoelectricity when antibody-antigen interaction occurred. Site-directed antibody was biotinylated after reducing the binding region to hinge region of randomly biotinylated antibody, which increased the number of effective binding sites. Investigating the effect of site-directed antibody on the cantilever sensor revealed that this protocol was able to enhance the performance of analytical sensitivity about 10 times with the modification of sensing layer. The experimental results demonstrated this biosensor can be used for diagnosis of acute myocardial infarction in clinical range of tens of ng/ml.

UR - http://www.scopus.com/inward/record.url?scp=33748052719&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748052719&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2005.11.011

DO - 10.1016/j.snb.2005.11.011

M3 - Article

AN - SCOPUS:33748052719

SN - 0925-4005

VL - 117

SP - 332

EP - 338

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

IS - 2

ER -

Label-free protein assay with site-directly immobilized antibody using self-actuating PZT cantilever

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this