Label-free detection of protein-protein interactions on multi-scale micro-well arrays using spatial light modulator

Eikhyun Cho; Ryung Shin; Jongmyeong Shim; Hyo Il Jung; Shinill Kang

doi:10.1063/1.4904006

Label-free detection of protein-protein interactions on multi-scale micro-well arrays using spatial light modulator

Eikhyun Cho, Ryung Shin, Jongmyeong Shim, Hyo Il Jung, Shinill Kang

Department of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

A mass production method of label-free protein microarray integrated with micro-well structures for the use of miniaturized multi-parallel scanning system was investigated. The geometrical parameters of biosensing structure were designed by rigorous coupled wave analysis simulation, and micro-well structures were designed considering the detection and material delivery system. The protein microarray with micro-well structures was fabricated by one-step UV nanoimprinting process using an electroformed multi-scale metallic stamp. Finally, microarray scanning was achieved using optical modulation without applying any motorized system and the feasibility of proposed protein microarray and scanning system was demonstrated by verifying the bio-molecular interactions.

Original language	English
Article number	231113
Journal	Applied Physics Letters
Volume	105
Issue number	23
DOIs	https://doi.org/10.1063/1.4904006
Publication status	Published - 2014 Dec 8

Bibliographical note

Publisher Copyright:
© 2014 AIP Publishing LLC.

All Science Journal Classification (ASJC) codes

Physics and Astronomy (miscellaneous)

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10.1063/1.4904006

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abstract = "A mass production method of label-free protein microarray integrated with micro-well structures for the use of miniaturized multi-parallel scanning system was investigated. The geometrical parameters of biosensing structure were designed by rigorous coupled wave analysis simulation, and micro-well structures were designed considering the detection and material delivery system. The protein microarray with micro-well structures was fabricated by one-step UV nanoimprinting process using an electroformed multi-scale metallic stamp. Finally, microarray scanning was achieved using optical modulation without applying any motorized system and the feasibility of proposed protein microarray and scanning system was demonstrated by verifying the bio-molecular interactions.",

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AU - Shim, Jongmyeong

AU - Jung, Hyo Il

AU - Kang, Shinill

PY - 2014/12/8

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AB - A mass production method of label-free protein microarray integrated with micro-well structures for the use of miniaturized multi-parallel scanning system was investigated. The geometrical parameters of biosensing structure were designed by rigorous coupled wave analysis simulation, and micro-well structures were designed considering the detection and material delivery system. The protein microarray with micro-well structures was fabricated by one-step UV nanoimprinting process using an electroformed multi-scale metallic stamp. Finally, microarray scanning was achieved using optical modulation without applying any motorized system and the feasibility of proposed protein microarray and scanning system was demonstrated by verifying the bio-molecular interactions.

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Label-free detection of protein-protein interactions on multi-scale micro-well arrays using spatial light modulator

Abstract

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