Label-free and direct detection of C-reactive protein using reduced graphene oxide-nanoparticle hybrid impedimetric sensor

Ajay Kumar Yagati; Jae Chul Pyun; Junhong Min; Sungbo Cho

doi:10.1016/j.bioelechem.2015.10.002

Label-free and direct detection of C-reactive protein using reduced graphene oxide-nanoparticle hybrid impedimetric sensor

Ajay Kumar Yagati, Jae Chul Pyun, Junhong Min, Sungbo Cho

Department of Materials Science and Engineering

Research output: Contribution to journal › Article › peer-review

78 Citations (Scopus)

Abstract

For label-free and direct detection of C-reactive protein (CRP), an impedimetric sensor based on an indium tin oxide (ITO) electrode array functionalized with reduced graphene oxide-nanoparticle (rGO-NP) hybrid was fabricated and evaluated. Analytical measurements were performed to examine the properties of rGO-NP-modified ITO microelectrodes and to determine the influence upon sensory performance of using nanostructures modified for antibody immobilization and for recognition of CRP binding events. Impedimetric measurements in the presence of the redox couple [Fe(CN)₆]^3-/4- showed significant changes in charge transfer resistance upon binding of CRP. The impedance measurements were highly target specific, linear with logarithmic CRP concentrations in PBS and human serum across a 1ngmL^-1 and 1000ngmL^-1 range and associated with a detection limits of 0.06 and 0.08ngmL^-1 respectively.

Original language	English
Pages (from-to)	37-44
Number of pages	8
Journal	Bioelectrochemistry
Volume	107
DOIs	https://doi.org/10.1016/j.bioelechem.2015.10.002
Publication status	Published - 2016 Feb 1

Bibliographical note

Funding Information:
This research was supported by the National Research Foundation of Korea (No. 2015-022008 , 2015-037739 ) and by the Korean Ministry of Trade, Industry & Energy (Industrial Convergence Promotion Business No. 10053803 ).

Publisher Copyright:
© 2015 Elsevier B.V.

All Science Journal Classification (ASJC) codes

Biophysics
Physical and Theoretical Chemistry
Electrochemistry

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10.1016/j.bioelechem.2015.10.002

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title = "Label-free and direct detection of C-reactive protein using reduced graphene oxide-nanoparticle hybrid impedimetric sensor",

abstract = "For label-free and direct detection of C-reactive protein (CRP), an impedimetric sensor based on an indium tin oxide (ITO) electrode array functionalized with reduced graphene oxide-nanoparticle (rGO-NP) hybrid was fabricated and evaluated. Analytical measurements were performed to examine the properties of rGO-NP-modified ITO microelectrodes and to determine the influence upon sensory performance of using nanostructures modified for antibody immobilization and for recognition of CRP binding events. Impedimetric measurements in the presence of the redox couple [Fe(CN)6]3-/4- showed significant changes in charge transfer resistance upon binding of CRP. The impedance measurements were highly target specific, linear with logarithmic CRP concentrations in PBS and human serum across a 1ngmL-1 and 1000ngmL-1 range and associated with a detection limits of 0.06 and 0.08ngmL-1 respectively.",

author = "Yagati, {Ajay Kumar} and Pyun, {Jae Chul} and Junhong Min and Sungbo Cho",

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doi = "10.1016/j.bioelechem.2015.10.002",

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AU - Yagati, Ajay Kumar

AU - Pyun, Jae Chul

AU - Min, Junhong

AU - Cho, Sungbo

N1 - Funding Information: This research was supported by the National Research Foundation of Korea (No. 2015-022008 , 2015-037739 ) and by the Korean Ministry of Trade, Industry & Energy (Industrial Convergence Promotion Business No. 10053803 ). Publisher Copyright: © 2015 Elsevier B.V.

PY - 2016/2/1

Y1 - 2016/2/1

N2 - For label-free and direct detection of C-reactive protein (CRP), an impedimetric sensor based on an indium tin oxide (ITO) electrode array functionalized with reduced graphene oxide-nanoparticle (rGO-NP) hybrid was fabricated and evaluated. Analytical measurements were performed to examine the properties of rGO-NP-modified ITO microelectrodes and to determine the influence upon sensory performance of using nanostructures modified for antibody immobilization and for recognition of CRP binding events. Impedimetric measurements in the presence of the redox couple [Fe(CN)6]3-/4- showed significant changes in charge transfer resistance upon binding of CRP. The impedance measurements were highly target specific, linear with logarithmic CRP concentrations in PBS and human serum across a 1ngmL-1 and 1000ngmL-1 range and associated with a detection limits of 0.06 and 0.08ngmL-1 respectively.

AB - For label-free and direct detection of C-reactive protein (CRP), an impedimetric sensor based on an indium tin oxide (ITO) electrode array functionalized with reduced graphene oxide-nanoparticle (rGO-NP) hybrid was fabricated and evaluated. Analytical measurements were performed to examine the properties of rGO-NP-modified ITO microelectrodes and to determine the influence upon sensory performance of using nanostructures modified for antibody immobilization and for recognition of CRP binding events. Impedimetric measurements in the presence of the redox couple [Fe(CN)6]3-/4- showed significant changes in charge transfer resistance upon binding of CRP. The impedance measurements were highly target specific, linear with logarithmic CRP concentrations in PBS and human serum across a 1ngmL-1 and 1000ngmL-1 range and associated with a detection limits of 0.06 and 0.08ngmL-1 respectively.

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Label-free and direct detection of C-reactive protein using reduced graphene oxide-nanoparticle hybrid impedimetric sensor

Abstract

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