Purpose: The potential of L-glutamine as a T2 exchange contrast agent in MRI was investigated. Methods: The T2 relaxation rate of L-glutamine solutions prepared in various concentrations was measured at 9.4 T. A series of T2-weighted images in a mouse cancer model was acquired with an L-glutamine solution infusion. Results: The T2 relaxivity caused by the exchange (R2ex) at 37°C was 0.069 s−1 mM−1 and 0.102 s−1 mM−1 for glutamine and glutamate solutions at pH = 7.2, respectively. The R2ex of glutamine at pH = 6.1–6.7 was in the 0.097–0.1 s−1 mM−1 range. No significant dependence of T1 on the concentration of glutamine was observed. The dynamic measurement of T2-weighted images in vivo showed that the glutamine uptake was primarily observed at the localized part of the tumor. Conclusion: L-glutamine can be used as a T2 exchange contrast agent and images of glutamine uptake in vivo can be acquired.
Bibliographical noteFunding Information:
C.G.J. is supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF‐2019R1I1A1A01064082).
© 2020 International Society for Magnetic Resonance in Medicine
All Science Journal Classification (ASJC) codes
- Radiology Nuclear Medicine and imaging