L-Ascorbic acid 2-phosphate and fibroblast growth factor-2 treatment maintains differentiation potential in bone marrow-derived mesenchymal stem cells through expression of hepatocyte growth factor

Sung Hae Bae; Hoon Ryu; Ki Jong Rhee; Ji Eun Oh; Soon Koo Baik; Kwang Yong Shim; Jee Hyun Kong; Shin Young Hyun; Hyun Sung Pack; Changjo Im; Ha Cheol Shin; Yong Man Kim; Hyun Soo Kim; Young Woo Eom; Jong In Lee

doi:10.3109/08977194.2015.1013628

L-Ascorbic acid 2-phosphate and fibroblast growth factor-2 treatment maintains differentiation potential in bone marrow-derived mesenchymal stem cells through expression of hepatocyte growth factor

Sung Hae Bae, Hoon Ryu, Ki Jong Rhee, Ji Eun Oh, Soon Koo Baik, Kwang Yong Shim, Jee Hyun Kong, Shin Young Hyun, Hyun Sung Pack, Changjo Im, Ha Cheol Shin, Yong Man Kim, Hyun Soo Kim, Young Woo Eom, Jong In Lee

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19 Citations (Scopus)

Abstract

l-ascorbic acid 2-phosphate (Asc-2P) acts as an antioxidant and a stimulator of hepatocyte growth factor (HGF) production. Previously, we reported that depletion of growth factors such as fibroblast growth factor (FGF)-2, epidermal growth factor (EGF), FGF-4 and HGF during serial passage could induce autophagy, senescence and down-regulation of stemness (proliferation via FGF-2/-4 and differentiation via HGF). In this study, we investigated the proliferation and differentiation potential of BMSCs by FGF-2 and Asc-2P. Co-treatment with FGF-2 and Asc-2P induced optimal proliferation of BMSCs and increased the accumulation rate of BMSC numbers during a 2-month culture period. Moreover, differentiation potential was maintained by co-treatment with FGF-2 and Asc-2P via HGF expression. Adipogenic differentiation potential by FGF-2 and Asc-2P was dramatically suppressed by c-Met inhibitors (SU11274). These data suggest that co-treatment with FGF-2 and Asc-2P would be beneficial in obtaining BMSCs that possess "stemness" during long-term culture.

Original language	English
Pages (from-to)	71-78
Number of pages	8
Journal	Growth Factors
Volume	33
Issue number	2
DOIs	https://doi.org/10.3109/08977194.2015.1013628
Publication status	Published - 2015 Apr 1

Bibliographical note

Funding Information:
The authors report no conflicts of interest. This research was partly supported by a research grant from Yonsei University Wonju College of Medicine (YUWCM-2005-02) and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2012R1A1A2004981).

Publisher Copyright:
© 2015 Informa UK Ltd. All rights reserved: reproduction in whole or part not permitted.

All Science Journal Classification (ASJC) codes

Endocrinology
Clinical Biochemistry
Cell Biology

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10.3109/08977194.2015.1013628

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Bae, S. H., Ryu, H., Rhee, K. J., Oh, J. E., Baik, S. K., Shim, K. Y., Kong, J. H., Hyun, S. Y., Pack, H. S., Im, C., Shin, H. C., Kim, Y. M., Kim, H. S., Eom, Y. W., & Lee, J. I. (2015). L-Ascorbic acid 2-phosphate and fibroblast growth factor-2 treatment maintains differentiation potential in bone marrow-derived mesenchymal stem cells through expression of hepatocyte growth factor. Growth Factors, 33(2), 71-78. https://doi.org/10.3109/08977194.2015.1013628

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title = "L-Ascorbic acid 2-phosphate and fibroblast growth factor-2 treatment maintains differentiation potential in bone marrow-derived mesenchymal stem cells through expression of hepatocyte growth factor",

abstract = "l-ascorbic acid 2-phosphate (Asc-2P) acts as an antioxidant and a stimulator of hepatocyte growth factor (HGF) production. Previously, we reported that depletion of growth factors such as fibroblast growth factor (FGF)-2, epidermal growth factor (EGF), FGF-4 and HGF during serial passage could induce autophagy, senescence and down-regulation of stemness (proliferation via FGF-2/-4 and differentiation via HGF). In this study, we investigated the proliferation and differentiation potential of BMSCs by FGF-2 and Asc-2P. Co-treatment with FGF-2 and Asc-2P induced optimal proliferation of BMSCs and increased the accumulation rate of BMSC numbers during a 2-month culture period. Moreover, differentiation potential was maintained by co-treatment with FGF-2 and Asc-2P via HGF expression. Adipogenic differentiation potential by FGF-2 and Asc-2P was dramatically suppressed by c-Met inhibitors (SU11274). These data suggest that co-treatment with FGF-2 and Asc-2P would be beneficial in obtaining BMSCs that possess {"}stemness{"} during long-term culture.",

author = "Bae, {Sung Hae} and Hoon Ryu and Rhee, {Ki Jong} and Oh, {Ji Eun} and Baik, {Soon Koo} and Shim, {Kwang Yong} and Kong, {Jee Hyun} and Hyun, {Shin Young} and Pack, {Hyun Sung} and Changjo Im and Shin, {Ha Cheol} and Kim, {Yong Man} and Kim, {Hyun Soo} and Eom, {Young Woo} and Lee, {Jong In}",

note = "Funding Information: The authors report no conflicts of interest. This research was partly supported by a research grant from Yonsei University Wonju College of Medicine (YUWCM-2005-02) and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2012R1A1A2004981). Publisher Copyright: {\textcopyright} 2015 Informa UK Ltd. All rights reserved: reproduction in whole or part not permitted.",

year = "2015",

month = apr,

day = "1",

doi = "10.3109/08977194.2015.1013628",

language = "English",

volume = "33",

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Bae, SH, Ryu, H, Rhee, KJ, Oh, JE, Baik, SK, Shim, KY, Kong, JH, Hyun, SY, Pack, HS, Im, C, Shin, HC, Kim, YM, Kim, HS, Eom, YW & Lee, JI 2015, 'L-Ascorbic acid 2-phosphate and fibroblast growth factor-2 treatment maintains differentiation potential in bone marrow-derived mesenchymal stem cells through expression of hepatocyte growth factor', Growth Factors, vol. 33, no. 2, pp. 71-78. https://doi.org/10.3109/08977194.2015.1013628

L-Ascorbic acid 2-phosphate and fibroblast growth factor-2 treatment maintains differentiation potential in bone marrow-derived mesenchymal stem cells through expression of hepatocyte growth factor. / Bae, Sung Hae; Ryu, Hoon; Rhee, Ki Jong et al.
In: Growth Factors, Vol. 33, No. 2, 01.04.2015, p. 71-78.

Research output: Contribution to journal › Article › peer-review

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T1 - L-Ascorbic acid 2-phosphate and fibroblast growth factor-2 treatment maintains differentiation potential in bone marrow-derived mesenchymal stem cells through expression of hepatocyte growth factor

AU - Bae, Sung Hae

AU - Ryu, Hoon

AU - Rhee, Ki Jong

AU - Oh, Ji Eun

AU - Baik, Soon Koo

AU - Shim, Kwang Yong

AU - Kong, Jee Hyun

AU - Hyun, Shin Young

AU - Pack, Hyun Sung

AU - Im, Changjo

AU - Shin, Ha Cheol

AU - Kim, Yong Man

AU - Kim, Hyun Soo

AU - Eom, Young Woo

AU - Lee, Jong In

N1 - Funding Information: The authors report no conflicts of interest. This research was partly supported by a research grant from Yonsei University Wonju College of Medicine (YUWCM-2005-02) and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2012R1A1A2004981). Publisher Copyright: © 2015 Informa UK Ltd. All rights reserved: reproduction in whole or part not permitted.

PY - 2015/4/1

Y1 - 2015/4/1

N2 - l-ascorbic acid 2-phosphate (Asc-2P) acts as an antioxidant and a stimulator of hepatocyte growth factor (HGF) production. Previously, we reported that depletion of growth factors such as fibroblast growth factor (FGF)-2, epidermal growth factor (EGF), FGF-4 and HGF during serial passage could induce autophagy, senescence and down-regulation of stemness (proliferation via FGF-2/-4 and differentiation via HGF). In this study, we investigated the proliferation and differentiation potential of BMSCs by FGF-2 and Asc-2P. Co-treatment with FGF-2 and Asc-2P induced optimal proliferation of BMSCs and increased the accumulation rate of BMSC numbers during a 2-month culture period. Moreover, differentiation potential was maintained by co-treatment with FGF-2 and Asc-2P via HGF expression. Adipogenic differentiation potential by FGF-2 and Asc-2P was dramatically suppressed by c-Met inhibitors (SU11274). These data suggest that co-treatment with FGF-2 and Asc-2P would be beneficial in obtaining BMSCs that possess "stemness" during long-term culture.

AB - l-ascorbic acid 2-phosphate (Asc-2P) acts as an antioxidant and a stimulator of hepatocyte growth factor (HGF) production. Previously, we reported that depletion of growth factors such as fibroblast growth factor (FGF)-2, epidermal growth factor (EGF), FGF-4 and HGF during serial passage could induce autophagy, senescence and down-regulation of stemness (proliferation via FGF-2/-4 and differentiation via HGF). In this study, we investigated the proliferation and differentiation potential of BMSCs by FGF-2 and Asc-2P. Co-treatment with FGF-2 and Asc-2P induced optimal proliferation of BMSCs and increased the accumulation rate of BMSC numbers during a 2-month culture period. Moreover, differentiation potential was maintained by co-treatment with FGF-2 and Asc-2P via HGF expression. Adipogenic differentiation potential by FGF-2 and Asc-2P was dramatically suppressed by c-Met inhibitors (SU11274). These data suggest that co-treatment with FGF-2 and Asc-2P would be beneficial in obtaining BMSCs that possess "stemness" during long-term culture.

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L-Ascorbic acid 2-phosphate and fibroblast growth factor-2 treatment maintains differentiation potential in bone marrow-derived mesenchymal stem cells through expression of hepatocyte growth factor

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