Ku in the cytoplasm associates with CD40 in human B cells and translocates into the nucleus following incubation with IL-4 and anti-CD40 mAb

Tomohiro Morio; Silva H. Hanissian; Leonard B. Bacharier; Hirobumi Teraoka; Shigeaki Nonoyama; Makoto Seki; Jun Kondo; Hiroyasu Nakano; Sang Kyou Lee; Raif S. Geha; Jun Ichi Yata

doi:10.1016/S1074-7613(00)80109-0

Ku in the cytoplasm associates with CD40 in human B cells and translocates into the nucleus following incubation with IL-4 and anti-CD40 mAb

Tomohiro Morio, Silva H. Hanissian, Leonard B. Bacharier, Hirobumi Teraoka, Shigeaki Nonoyama, Makoto Seki, Jun Kondo, Hiroyasu Nakano, Sang Kyou Lee, Raif S. Geha, Jun Ichi Yata

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

62 Citations (Scopus)

Abstract

CD40 plays a critical role in survival, growth, differentiation, and class switching of B lymphocytes. Although Ku is required for immunoglobulin class switching, how CD40 signal transduction is coupled to Ku is still unknown. Here, we show that CD40 directly interacts with Ku through the membrane-proximal region of cytoplasmic CD40. Ku was confined to the cytoplasm in human primary B cells, and the engagement of CD40 on the B cells cultured in the presence of IL-4 resulted in the dissociation of Ku from CD40, translocation of Ku into the nucleus, and increase in the activity of DNA-dependent protein kinase. These findings indicate that Ku is involved in the CD40 signal transduction pathway and may play an important role in the CD40-mediated events.

Original language	English
Pages (from-to)	339-348
Number of pages	10
Journal	Immunity
Volume	11
Issue number	3
DOIs	https://doi.org/10.1016/S1074-7613(00)80109-0
Publication status	Published - 1999 Sept

Bibliographical note

Funding Information:
We thank B. Seed and T. Watanabe for cDNA constructs; E. A. Clark, S. M. Fu, T. Kobata, and T. Mimori for antibodies; K. Moore for IL-4: and all the members of R. S. G.'s lab, where this work was initiated. This work was supported by grants from Kato Memorial Foundation (T. M.) and from the Ministry of Education, Science, Sports, and Culture of Japan (T. M., H. T., and J. Y.).

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S1074-7613(00)80109-0

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@article{3aa1f398b283401f9b2f8f320720a314,

title = "Ku in the cytoplasm associates with CD40 in human B cells and translocates into the nucleus following incubation with IL-4 and anti-CD40 mAb",

abstract = "CD40 plays a critical role in survival, growth, differentiation, and class switching of B lymphocytes. Although Ku is required for immunoglobulin class switching, how CD40 signal transduction is coupled to Ku is still unknown. Here, we show that CD40 directly interacts with Ku through the membrane-proximal region of cytoplasmic CD40. Ku was confined to the cytoplasm in human primary B cells, and the engagement of CD40 on the B cells cultured in the presence of IL-4 resulted in the dissociation of Ku from CD40, translocation of Ku into the nucleus, and increase in the activity of DNA-dependent protein kinase. These findings indicate that Ku is involved in the CD40 signal transduction pathway and may play an important role in the CD40-mediated events.",

author = "Tomohiro Morio and Hanissian, {Silva H.} and Bacharier, {Leonard B.} and Hirobumi Teraoka and Shigeaki Nonoyama and Makoto Seki and Jun Kondo and Hiroyasu Nakano and Lee, {Sang Kyou} and Geha, {Raif S.} and Yata, {Jun Ichi}",

note = "Funding Information: We thank B. Seed and T. Watanabe for cDNA constructs; E. A. Clark, S. M. Fu, T. Kobata, and T. Mimori for antibodies; K. Moore for IL-4: and all the members of R. S. G.'s lab, where this work was initiated. This work was supported by grants from Kato Memorial Foundation (T. M.) and from the Ministry of Education, Science, Sports, and Culture of Japan (T. M., H. T., and J. Y.).",

year = "1999",

month = sep,

doi = "10.1016/S1074-7613(00)80109-0",

language = "English",

volume = "11",

pages = "339--348",

journal = "Immunity",

issn = "1074-7613",

publisher = "Cell Press",

number = "3",

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TY - JOUR

T1 - Ku in the cytoplasm associates with CD40 in human B cells and translocates into the nucleus following incubation with IL-4 and anti-CD40 mAb

AU - Morio, Tomohiro

AU - Hanissian, Silva H.

AU - Bacharier, Leonard B.

AU - Teraoka, Hirobumi

AU - Nonoyama, Shigeaki

AU - Seki, Makoto

AU - Kondo, Jun

AU - Nakano, Hiroyasu

AU - Lee, Sang Kyou

AU - Geha, Raif S.

AU - Yata, Jun Ichi

N1 - Funding Information: We thank B. Seed and T. Watanabe for cDNA constructs; E. A. Clark, S. M. Fu, T. Kobata, and T. Mimori for antibodies; K. Moore for IL-4: and all the members of R. S. G.'s lab, where this work was initiated. This work was supported by grants from Kato Memorial Foundation (T. M.) and from the Ministry of Education, Science, Sports, and Culture of Japan (T. M., H. T., and J. Y.).

PY - 1999/9

Y1 - 1999/9

N2 - CD40 plays a critical role in survival, growth, differentiation, and class switching of B lymphocytes. Although Ku is required for immunoglobulin class switching, how CD40 signal transduction is coupled to Ku is still unknown. Here, we show that CD40 directly interacts with Ku through the membrane-proximal region of cytoplasmic CD40. Ku was confined to the cytoplasm in human primary B cells, and the engagement of CD40 on the B cells cultured in the presence of IL-4 resulted in the dissociation of Ku from CD40, translocation of Ku into the nucleus, and increase in the activity of DNA-dependent protein kinase. These findings indicate that Ku is involved in the CD40 signal transduction pathway and may play an important role in the CD40-mediated events.

AB - CD40 plays a critical role in survival, growth, differentiation, and class switching of B lymphocytes. Although Ku is required for immunoglobulin class switching, how CD40 signal transduction is coupled to Ku is still unknown. Here, we show that CD40 directly interacts with Ku through the membrane-proximal region of cytoplasmic CD40. Ku was confined to the cytoplasm in human primary B cells, and the engagement of CD40 on the B cells cultured in the presence of IL-4 resulted in the dissociation of Ku from CD40, translocation of Ku into the nucleus, and increase in the activity of DNA-dependent protein kinase. These findings indicate that Ku is involved in the CD40 signal transduction pathway and may play an important role in the CD40-mediated events.

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AN - SCOPUS:0033199987

SN - 1074-7613

VL - 11

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EP - 348

JO - Immunity

JF - Immunity

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ER -

Ku in the cytoplasm associates with CD40 in human B cells and translocates into the nucleus following incubation with IL-4 and anti-CD40 mAb

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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