Isolation of adipose-derived stem cells by using a subfractionation culturing method

Tac Ghee Yi, Wang Kyun Kim, Joon Seok Choi, Seung Yong Song, Juhee Han, Ji Hye Kim, Won Serk Kim, Sang Gyu Park, Hyun Joo Lee, Yun Kyoung Cho, Sung Joo Hwang, Sun U. Song, Jong Hyuk Sung

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


Objective: Adipose-derived stem cells (ASCs) isolated from subcutaneous adipose tissue have been tested in clinical trials. However, ASCs isolated by enzyme digestion and centrifugation are heterogeneous and exhibit wide variation in regenerative potential and clinical outcomes. Therefore, we developed a new method for isolating clonal ASCs (cASCs) that does not use enzyme digestion or centrifugation steps. Research design and methods: In addition to cell surface markers and differentiation potential, we compared the mitogenic, paracrine and hair growth-promoting effects of ASCs isolated by the gradient centrifugation method (GCM) or by the new subfractionation culturing method (SCM). Results: We selected three cASCs isolated by SCM that showed high rates of proliferation. The cell surface markers expressed by ASCs isolated by GCM or SCM were very similar, and SCM-isolated ASCs could potentially differentiate into different cell lineages. However, cASC lines exhibited better mitogenic and paracrine effects than ASCs isolated by GCM. The expression of Diras3, Myb, Cdca7, Mki67, Rrm2, Cdk1 and Ccna2, which may play a key role in cASC proliferation, was upregulated in cASCs. In addition, cASCs exhibited enhanced hair growth-promoting effects in dermal papilla cells and animal experiments. Conclusions: SCM generates a highly homogeneous population of ASCs via a simple and effective procedure that can be used in therapeutic settings.

Original languageEnglish
Pages (from-to)1551-1560
Number of pages10
JournalExpert Opinion on Biological Therapy
Issue number11
Publication statusPublished - 2014 Nov 1

Bibliographical note

Publisher Copyright:
© 2014 Informa UK, Ltd.

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry


Dive into the research topics of 'Isolation of adipose-derived stem cells by using a subfractionation culturing method'. Together they form a unique fingerprint.

Cite this