Isolation of a novel Lactiplantibacillus plantarum strain resistant to nitrite stress and its transcriptome analysis

Chae Young Kwon, Kyoung Jin Choi, Dongeun Yong, Ji Eun Kim, Sang Sun Yoon

Research output: Contribution to journalArticlepeer-review


Nitric oxide (NO) is a reactive nitrogen species (RNS) that plays a vital role in regulating inflammatory processes. Under abnormal conditions, excessive NO levels can promote the oxidation of cellular components, which may cause or exacerbate diseases such as hypertension, cardiovascular dysfunction, and inflammatory bowel disease (IBD). Previous studies have shown that reducing NO levels in the lumen can attenuate the clinical symptoms of IBD. Thus, we aimed to identify bacteria that can reduce RNS and that can be used as valuable probiotics. In this study, we isolated bacteria resistant to nitrite stress from human feces and used 16S and whole-genome sequencing to identify them as Lactiplantibacillus plantarum LP7 (LP7). The ability to survive at high nitrite levels and to decrease them was greater in the LP7 strain than in the reference strain L. plantarum ATCC14917 (ATCC14917). To characterize the LP7 genome in more detail, we performed a comparative genome analysis. However, the unique genes that directly confer the ability to withstand nitrite stress were not present in the LP7 genome. Furthermore, we performed transcriptomic analysis of LP7 and ATCC14917 cells treated with nitrite. We found that the expression levels of genes involved in the cell division process were induced in LP7, which showed a more regular rod-shape than ATCC14917. This could explain why LP7 can survive better than ATCC14917 under nitrite stress. Based on its ability to survive better in nitrite stress and decrease nitrite concentration, we suggest that LP7 could be a valuable probiotic strain.

Original languageEnglish
Pages (from-to)715-726
Number of pages12
JournalJournal of Microbiology
Issue number7
Publication statusPublished - 2022 Jul

Bibliographical note

Funding Information:
This study was supported by the Basic Science Research Program through the National Research foundation of Korea (NRF) funded by the Ministry of Education (Grant No. NRF-2020R1A6A3A01097647, to Ji-Eun Kim). This work was also supported by grants from the National Research Foundation (NRF) of Korea (2019R1A6A1A03032869, to Sang Sun Yoon). This research was also supported by a grant from the Korea Health Technology R&D Project funded by the Ministry of Health & Welfare, Korea (HI14C1324, to Sang Sun Yoon).

Publisher Copyright:
© 2022, Author(s).

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Applied Microbiology and Biotechnology


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