TY - JOUR
T1 - Isolating bronchial epithelial cell preparations from gross lung specimens
AU - Kwon, Yong Jae
AU - Lee, Seog Joo
AU - Koh, Jae Soo
AU - Kim, Young Joon
AU - Park, Jong Ho
PY - 2009/9
Y1 - 2009/9
N2 - Tissue microdissection is appropriate for separating pure cells from heterogeneous tissues. Recently, we have focused on whole genome DNA methylation patterns of lung squamous cell carcinoma (SCC) and needed to obtain the appropriate counterpart cells of lung SCC. However, in some regions of human tissues, such as in bronchial epithelium, it is difficult to apply tissue microdissection as a means to isolate pure cells from a heterogenous mixture of cells. Accordingly, we developed the pop brush method to retrieve sufficient amounts of pure bronchial epithelium from gross lung specimens, and this method enables us to study epigenetic variations of lung SCC.
AB - Tissue microdissection is appropriate for separating pure cells from heterogeneous tissues. Recently, we have focused on whole genome DNA methylation patterns of lung squamous cell carcinoma (SCC) and needed to obtain the appropriate counterpart cells of lung SCC. However, in some regions of human tissues, such as in bronchial epithelium, it is difficult to apply tissue microdissection as a means to isolate pure cells from a heterogenous mixture of cells. Accordingly, we developed the pop brush method to retrieve sufficient amounts of pure bronchial epithelium from gross lung specimens, and this method enables us to study epigenetic variations of lung SCC.
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U2 - 10.1007/s11626-009-9229-y
DO - 10.1007/s11626-009-9229-y
M3 - Article
C2 - 19609830
AN - SCOPUS:70349332929
SN - 1071-2690
VL - 45
SP - 496
EP - 499
JO - In Vitro Cellular and Developmental Biology - Animal
JF - In Vitro Cellular and Developmental Biology - Animal
IS - 9
ER -