Involvement of PI3K and PKA pathways in mouse tongue epithelial differentiation

Jae Kwang Jung, Hye In Jung, Sanjiv Neupane, Ki Rim Kim, Ji Youn Kim, Hitoshi Yamamoto, Sung Won Cho, Youngkyun Lee, Hong In Shin, Wern Joo Sohn, Jae Young Kim

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


In mice, tongue epithelial differentiation is mainly regulated by the interactions among various signalling molecules including Fgf signalling pathways. However, the subsequent signalling modulations for epithelial maturation, initiated by Fgf signalling, remain to be elucidated. Therefore, we employed an in vitro tongue organ cultivation system along with the applications of various pharmacological inhibitors against the intracellular signalling molecules of Fgf signalling pathways, including H89, LY294002, PD98059, and U0126. Following treatments with LY294002 and H89, inhibitors for PI3K and PKA, respectively, the decreased thickness of the tongue epithelium was observed along with the alteration in cell proliferative and apoptotic patterns. Meanwhile, cultivated tongues treated with MEK inhibitor U0126 or PD98059 showed significantly decreased cell proliferation in the tongue epithelium and the mesenchyme. Based on these results, we suggest that the tongue epithelium is differentiated into multiple epithelial cell layers via the PI3K and PKA pathways in tissue-specific manner during the epithelial-mesenchymal interactions.

Original languageEnglish
Pages (from-to)92-98
Number of pages7
JournalActa Histochemica
Issue number1
Publication statusPublished - 2017 Jan 1

Bibliographical note

Publisher Copyright:
© 2016 Elsevier GmbH

All Science Journal Classification (ASJC) codes

  • Histology
  • Cell Biology


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