Involvement of phospholipase D in oxidative stress-induced necrosis of vascular smooth muscle cells

Eun Young Shin; Do Sik Min; Ji Cheol Shin; Kyung Sun Shin; Min Soo Hyun; Kwon Soo Ha; Hun Sik Kim; Hee Yul Ahn; Eung Gook Kim

doi:10.1016/S0014-5793(01)03059-9

Involvement of phospholipase D in oxidative stress-induced necrosis of vascular smooth muscle cells

Eun Young Shin, Do Sik Min, Ji Cheol Shin, Kyung Sun Shin, Min Soo Hyun, Kwon Soo Ha, Hun Sik Kim, Hee Yul Ahn, Eung Gook Kim

Department of Pharmacy

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Phospholipase D (PLD) has been associated with necrosis. However, it is not clear whether PLD plays a causative role in this cellular process. We investigated the role of PLD in oxidative stress-induced necrosis of vascular smooth muscle cells (VSMCs). Pervanadate (hydrogen peroxide plus orthovanadate) but not hydrogen peroxide alone activated PLD in a dose- and time-dependent manner. Exposure of VSMCs to pervanadate resulted in necrosis. Pretreatment with butan-1-ol, a PLD inhibitor, attenuated both pervanadate-induced necrosis and increase of intracellular Ca²⁺. Removal of extracellular Ca²⁺ inhibited pervanadate-induced necrosis by 50%. These results suggest that PLD activation mediates pervanadate-induced necrosis of VSMCs, which is at least partly due to Ca²⁺ toxicity.

Original language	English
Pages (from-to)	277-281
Number of pages	5
Journal	FEBS Letters
Volume	508
Issue number	2
DOIs	https://doi.org/10.1016/S0014-5793(01)03059-9
Publication status	Published - 2001 Nov 16

Bibliographical note

Funding Information:
This work was supported by the Korean Research Foundation Grants (KRF-1999-015-FP0062 and FP0097).

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(01)03059-9

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title = "Involvement of phospholipase D in oxidative stress-induced necrosis of vascular smooth muscle cells",

abstract = "Phospholipase D (PLD) has been associated with necrosis. However, it is not clear whether PLD plays a causative role in this cellular process. We investigated the role of PLD in oxidative stress-induced necrosis of vascular smooth muscle cells (VSMCs). Pervanadate (hydrogen peroxide plus orthovanadate) but not hydrogen peroxide alone activated PLD in a dose- and time-dependent manner. Exposure of VSMCs to pervanadate resulted in necrosis. Pretreatment with butan-1-ol, a PLD inhibitor, attenuated both pervanadate-induced necrosis and increase of intracellular Ca2+. Removal of extracellular Ca2+ inhibited pervanadate-induced necrosis by 50%. These results suggest that PLD activation mediates pervanadate-induced necrosis of VSMCs, which is at least partly due to Ca2+ toxicity.",

author = "Shin, {Eun Young} and Min, {Do Sik} and Shin, {Ji Cheol} and Shin, {Kyung Sun} and Hyun, {Min Soo} and Ha, {Kwon Soo} and Kim, {Hun Sik} and Ahn, {Hee Yul} and Kim, {Eung Gook}",

note = "Funding Information: This work was supported by the Korean Research Foundation Grants (KRF-1999-015-FP0062 and FP0097).",

year = "2001",

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doi = "10.1016/S0014-5793(01)03059-9",

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T1 - Involvement of phospholipase D in oxidative stress-induced necrosis of vascular smooth muscle cells

AU - Shin, Eun Young

AU - Min, Do Sik

AU - Shin, Ji Cheol

AU - Shin, Kyung Sun

AU - Hyun, Min Soo

AU - Ha, Kwon Soo

AU - Kim, Hun Sik

AU - Ahn, Hee Yul

AU - Kim, Eung Gook

N1 - Funding Information: This work was supported by the Korean Research Foundation Grants (KRF-1999-015-FP0062 and FP0097).

PY - 2001/11/16

Y1 - 2001/11/16

N2 - Phospholipase D (PLD) has been associated with necrosis. However, it is not clear whether PLD plays a causative role in this cellular process. We investigated the role of PLD in oxidative stress-induced necrosis of vascular smooth muscle cells (VSMCs). Pervanadate (hydrogen peroxide plus orthovanadate) but not hydrogen peroxide alone activated PLD in a dose- and time-dependent manner. Exposure of VSMCs to pervanadate resulted in necrosis. Pretreatment with butan-1-ol, a PLD inhibitor, attenuated both pervanadate-induced necrosis and increase of intracellular Ca2+. Removal of extracellular Ca2+ inhibited pervanadate-induced necrosis by 50%. These results suggest that PLD activation mediates pervanadate-induced necrosis of VSMCs, which is at least partly due to Ca2+ toxicity.

AB - Phospholipase D (PLD) has been associated with necrosis. However, it is not clear whether PLD plays a causative role in this cellular process. We investigated the role of PLD in oxidative stress-induced necrosis of vascular smooth muscle cells (VSMCs). Pervanadate (hydrogen peroxide plus orthovanadate) but not hydrogen peroxide alone activated PLD in a dose- and time-dependent manner. Exposure of VSMCs to pervanadate resulted in necrosis. Pretreatment with butan-1-ol, a PLD inhibitor, attenuated both pervanadate-induced necrosis and increase of intracellular Ca2+. Removal of extracellular Ca2+ inhibited pervanadate-induced necrosis by 50%. These results suggest that PLD activation mediates pervanadate-induced necrosis of VSMCs, which is at least partly due to Ca2+ toxicity.

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ER -

Involvement of phospholipase D in oxidative stress-induced necrosis of vascular smooth muscle cells

Abstract

Bibliographical note

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