TY - JOUR
T1 - Investigation of extended-spectrum β-lactamases produced by clinical isolates of Klebsiella pneumoniae and Escherichia coli in Korea
AU - Jeong, S. H.
AU - Bae, I. K.
AU - Kwon, S. B.
AU - Lee, J. H.
AU - Jung, H. I.
AU - Song, J. S.
AU - Jeong, B. C.
AU - Kim, S. J.
AU - Lee, S. H.
PY - 2004
Y1 - 2004
N2 - Aims: Isolates obtained from various regions in Korea in 2002 were identified and their susceptibility to extended-spectrum cephalosporins, monobactams and/or cephamycins was studied along with any production of extended-spectrum β-lactamases (ESBLs). Methods and Results: Bacteria identified by the conventional techniques and Vitek GNI card were Klebsiella pneumoniae and Escherichia coli. Using disk diffusion and double-disk synergy tests, we found that 39.2% of strains produced ESBLs. About 52% of isolates transferred resistance to ceftazidime by conjugation. Banding patterns of PCR amplification with the designed primers showed that 837- and 259-bp fragments specific to blaTEM genes were amplified in 63.3% of strains. 929- and 231-bp fragments (blaSHV), 847- and 520-bp fragments (bla CMY), 597- and 858-bp fragments (blaCTX-M) were amplified in 61.5, 17.3 and 7.7% of strains respectively. About 51.9% of strains contained more than two types of β-lactamase genes. Especially, one strain contained blaTEM, blaCMY and blaCTX-M genes. Significance: Resistance mechanisms to β-lactams, comprising mostly ESBL production, lead to the resistance against even recently developed β-lactams in enterobacteria, which is now a serious threat to antibiotic therapy. The high prevalence of blaCMY genes and multidrug-resistant genes may also make therapeutic failure and lack of eradiation of these strains by extended-spectrum cephalosporins or cephamycins.
AB - Aims: Isolates obtained from various regions in Korea in 2002 were identified and their susceptibility to extended-spectrum cephalosporins, monobactams and/or cephamycins was studied along with any production of extended-spectrum β-lactamases (ESBLs). Methods and Results: Bacteria identified by the conventional techniques and Vitek GNI card were Klebsiella pneumoniae and Escherichia coli. Using disk diffusion and double-disk synergy tests, we found that 39.2% of strains produced ESBLs. About 52% of isolates transferred resistance to ceftazidime by conjugation. Banding patterns of PCR amplification with the designed primers showed that 837- and 259-bp fragments specific to blaTEM genes were amplified in 63.3% of strains. 929- and 231-bp fragments (blaSHV), 847- and 520-bp fragments (bla CMY), 597- and 858-bp fragments (blaCTX-M) were amplified in 61.5, 17.3 and 7.7% of strains respectively. About 51.9% of strains contained more than two types of β-lactamase genes. Especially, one strain contained blaTEM, blaCMY and blaCTX-M genes. Significance: Resistance mechanisms to β-lactams, comprising mostly ESBL production, lead to the resistance against even recently developed β-lactams in enterobacteria, which is now a serious threat to antibiotic therapy. The high prevalence of blaCMY genes and multidrug-resistant genes may also make therapeutic failure and lack of eradiation of these strains by extended-spectrum cephalosporins or cephamycins.
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U2 - 10.1111/j.1472-765X.2004.01537.x
DO - 10.1111/j.1472-765X.2004.01537.x
M3 - Article
C2 - 15189286
AN - SCOPUS:3042778914
SN - 0266-8254
VL - 39
SP - 41
EP - 47
JO - Letters in Applied Microbiology
JF - Letters in Applied Microbiology
IS - 1
ER -