TY - GEN
T1 - Investigation of 3-D cell-based assays with structured illumination using a digital micromirror device
AU - Choi, Jong Ryul
AU - Kim, Donghyun
PY - 2012
Y1 - 2012
N2 - In this paper, we demonstrate a fluorescence optical detection system with structured illumination using a digital micromirror device to investigate various cell-based assays. To study the performance of the optical detection system, we measured 2-D and 3-D assays of fluorescent microbeads and extended the results with mammalian cells. Through image reconstruction after structured illumination, we have improved fluorescence images by 32 times in terms of the size of axial point-spread function. The findings can be useful to supplement structured illumination microscopy that has been actively investigated for various applications.
AB - In this paper, we demonstrate a fluorescence optical detection system with structured illumination using a digital micromirror device to investigate various cell-based assays. To study the performance of the optical detection system, we measured 2-D and 3-D assays of fluorescent microbeads and extended the results with mammalian cells. Through image reconstruction after structured illumination, we have improved fluorescence images by 32 times in terms of the size of axial point-spread function. The findings can be useful to supplement structured illumination microscopy that has been actively investigated for various applications.
UR - http://www.scopus.com/inward/record.url?scp=85087193062&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85087193062&partnerID=8YFLogxK
U2 - 10.1364/fio.2012.fm4d.3
DO - 10.1364/fio.2012.fm4d.3
M3 - Conference contribution
AN - SCOPUS:85087193062
SN - 9781557529565
T3 - Frontiers in Optics, FIO 2012
BT - Frontiers in Optics, FIO 2012
PB - Optical Society of America (OSA)
T2 - Frontiers in Optics, FIO 2012
Y2 - 14 October 2012 through 18 October 2012
ER -