Intracellular ca2+-mediated ae2 is involved in the vectorial movement of hacat keratinocyte

Soyoung Hwang; Dong Min Shin; Jeong Hee Hong

doi:10.3390/ijms21228429

Intracellular ca²⁺-mediated ae2 is involved in the vectorial movement of hacat keratinocyte

Soyoung Hwang, Dong Min Shin, Jeong Hee Hong

Department of Oral Biology

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Keratinocyte migration is initiated toward the wound skin barrier as a crucial process in wound healing. However, the migratory machinery used by keratinocytes is relatively unknown. Histamine signaling, including an increase in the Ca²⁺ signal, mediated the enhanced protein expression and chloride/bicarbonate exchange activity of anion exchanger AE2 in keratinocytes. In this study, we applied an agarose spot assay to induce a vectorial motion. The vectorial stimulation of the histamine-containing agarose spot enhanced the HaCaT keratinocyte migration, compared to non-directional stimulation. AE2 is associated with the vectorial movement of HaCaT keratinocytes. Enhanced expression of AE2 was mainly associated with an increase in Ca²⁺ and was abolished by the treatment with the Ca²⁺ chelating agent BAPTA-AM. These findings revealed that the directionality of Ca²⁺-exerted stimulation can play a prominent role in facilitating migration through the involvement of AE2 as a migratory machinery in HaCaT keratinocytes.

Original language	English
Article number	8429
Pages (from-to)	1-17
Number of pages	17
Journal	International journal of molecular sciences
Volume	21
Issue number	22
DOIs	https://doi.org/10.3390/ijms21228429
Publication status	Published - 2020 Nov 2

Bibliographical note

Funding Information:
Funding: This work was supported by National Research Foundation of Korea (NRF) grants funded by the Korean government (MSIT; NRF-2019R1F1A1046785 (J.H.H.) and NRF-2020R1A2C2003409 (D.M.S.)).

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

All Science Journal Classification (ASJC) codes

Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry

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10.3390/ijms21228429

Cite this

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title = "Intracellular ca2+-mediated ae2 is involved in the vectorial movement of hacat keratinocyte",

abstract = "Keratinocyte migration is initiated toward the wound skin barrier as a crucial process in wound healing. However, the migratory machinery used by keratinocytes is relatively unknown. Histamine signaling, including an increase in the Ca2+ signal, mediated the enhanced protein expression and chloride/bicarbonate exchange activity of anion exchanger AE2 in keratinocytes. In this study, we applied an agarose spot assay to induce a vectorial motion. The vectorial stimulation of the histamine-containing agarose spot enhanced the HaCaT keratinocyte migration, compared to non-directional stimulation. AE2 is associated with the vectorial movement of HaCaT keratinocytes. Enhanced expression of AE2 was mainly associated with an increase in Ca2+ and was abolished by the treatment with the Ca2+ chelating agent BAPTA-AM. These findings revealed that the directionality of Ca2+-exerted stimulation can play a prominent role in facilitating migration through the involvement of AE2 as a migratory machinery in HaCaT keratinocytes.",

author = "Soyoung Hwang and Shin, {Dong Min} and Hong, {Jeong Hee}",

note = "Funding Information: Funding: This work was supported by National Research Foundation of Korea (NRF) grants funded by the Korean government (MSIT; NRF-2019R1F1A1046785 (J.H.H.) and NRF-2020R1A2C2003409 (D.M.S.)). Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",

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N2 - Keratinocyte migration is initiated toward the wound skin barrier as a crucial process in wound healing. However, the migratory machinery used by keratinocytes is relatively unknown. Histamine signaling, including an increase in the Ca2+ signal, mediated the enhanced protein expression and chloride/bicarbonate exchange activity of anion exchanger AE2 in keratinocytes. In this study, we applied an agarose spot assay to induce a vectorial motion. The vectorial stimulation of the histamine-containing agarose spot enhanced the HaCaT keratinocyte migration, compared to non-directional stimulation. AE2 is associated with the vectorial movement of HaCaT keratinocytes. Enhanced expression of AE2 was mainly associated with an increase in Ca2+ and was abolished by the treatment with the Ca2+ chelating agent BAPTA-AM. These findings revealed that the directionality of Ca2+-exerted stimulation can play a prominent role in facilitating migration through the involvement of AE2 as a migratory machinery in HaCaT keratinocytes.

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