Intracellular ca2+-mediated ae2 is involved in the vectorial movement of hacat keratinocyte

Soyoung Hwang, Dong Min Shin, Jeong Hee Hong

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7 Citations (Scopus)


Keratinocyte migration is initiated toward the wound skin barrier as a crucial process in wound healing. However, the migratory machinery used by keratinocytes is relatively unknown. Histamine signaling, including an increase in the Ca2+ signal, mediated the enhanced protein expression and chloride/bicarbonate exchange activity of anion exchanger AE2 in keratinocytes. In this study, we applied an agarose spot assay to induce a vectorial motion. The vectorial stimulation of the histamine-containing agarose spot enhanced the HaCaT keratinocyte migration, compared to non-directional stimulation. AE2 is associated with the vectorial movement of HaCaT keratinocytes. Enhanced expression of AE2 was mainly associated with an increase in Ca2+ and was abolished by the treatment with the Ca2+ chelating agent BAPTA-AM. These findings revealed that the directionality of Ca2+-exerted stimulation can play a prominent role in facilitating migration through the involvement of AE2 as a migratory machinery in HaCaT keratinocytes.

Original languageEnglish
Article number8429
Pages (from-to)1-17
Number of pages17
JournalInternational journal of molecular sciences
Issue number22
Publication statusPublished - 2020 Nov 2

Bibliographical note

Funding Information:
Funding: This work was supported by National Research Foundation of Korea (NRF) grants funded by the Korean government (MSIT; NRF-2019R1F1A1046785 (J.H.H.) and NRF-2020R1A2C2003409 (D.M.S.)).

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry


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