Interspecies microbial nexus facilitated methanation of polysaccharidic wastes

Shouvik Saha, Byong Hun Jeon, Mayur B. Kurade, Sanjay P. Govindwar, Pradip K. Chatterjee, Sang Eun Oh, Hyun Seog Roh, Sean S. Lee

Research output: Contribution to journalArticlepeer-review

52 Citations (Scopus)


Compositional variations in organic wastes influence microbial abundancy and syntrophy during anaerobic digestion (AD), impacting the normal performance of digesters for methanation. Investigation of the microbial dynamics during AD following augmentation with polysaccharidic wastes (PW) revealed the association of effective digester performance and methane yields with the microbial nexus. Dominance of the acidogenic saccharolytic genera, Prevotella, Eubacterium, and Lachnoclostridium, enhanced the utilization of carbohydrates (54%) in PW-augmented digesters. Spearman's rs correlation showed dynamic interspecies interactions among acetogenic syntrophs, and that of iron oxidizers/reducers with acetoclastic and hydrogenotrophic methanogens. Propionate oxidizers in Chloroflexi (i.e., Bellilinea, Levilinea, and Longilinea) exhibited positive associations with acetoclastic methanogens. Increase in the population of acetoclastic methanogens (Methanosaeta, 77% and Methanosarcina, 9%) accelerated the methanogenic activity of PW-augmented digesters by 7 times during the exponential phase, increasing the methane yield (75%) compared to the control. Thus, microbial syntrophy facilitated the effective methanation of PW during AD process.

Original languageEnglish
Article number121638
JournalBioresource technology
Publication statusPublished - 2019 Oct

Bibliographical note

Funding Information:
This work was supported by the Korea Institute of Energy Technology Evaluation and Planning (KETEP) and the Ministry of Trade, Industry & Energy ( MOTIE ) of the Republic of Korea (No. 20163010092250 ; No. 20173010092470 ).

Publisher Copyright:
© 2019 Elsevier Ltd

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


Dive into the research topics of 'Interspecies microbial nexus facilitated methanation of polysaccharidic wastes'. Together they form a unique fingerprint.

Cite this