Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques

Martin Cox; So Kawaguchi; Robert King; Kishan Dholakia; Christian T.A. Brown

doi:10.1080/10236244.2015.1073455

Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques

Martin Cox, So Kawaguchi, Robert King, Kishan Dholakia, Christian T.A. Brown

Department of Physics

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The accurate observation of physiological changes on in vivo samples of important animal species such as Euphausia superba (Antarctic krill) is an important goal in helping to understand how environmental changes can affect animal development. Using a custom made ‘krill trap’, live un-anaesthetized krill were confined for seven hours, during which three hours of optical imaging were obtained and no subsequent ill effects observed. The trap enabled two imaging methods to be employed: optical coherence tomography (OCT) and microscopy. OCT enabled internal structure and tissues to be imaged to a depth of approximately 2 mm and resolution of approximately 12 μm. Microscopy was used to observe heart rate. During our experiments, we imaged a range of internal structures in live animals including the heart and gastric areas. The trap design enables a new generation of mixed modality imaging of these animals in vivo. These techniques will enable detailed studies of the internal physiology of live krill to be undertaken under a wide range of environmental conditions and have the potential to highlight important variations in behaviour and animal development.

Original language	English
Pages (from-to)	455-466
Number of pages	12
Journal	Marine and Freshwater Behaviour and Physiology
Volume	48
Issue number	6
DOIs	https://doi.org/10.1080/10236244.2015.1073455
Publication status	Published - 2015 Nov 2

Bibliographical note

Funding Information:
The collection and rearing of krill specimens was funded by the Australian Antarctic Division Science Programme Project 4037 (Experimental Krill Biology: Response of krill to environmental change), and Project 4050 (Assessing change in krill distribution and abundance in Eastern Antarctica). MJC is funded by an Australian Research Council grant FS110200057. CTAB acknowledges support for instrument development and shipping costs from the United Kingdom Engineering and Physical Sciences Research Council Grant EP/M000869/1 (Shaped Light at the Interface). Live wild krill were caught in the Southern Ocean under permits issued by the Australian Department of Environment and Heritage under the Environment Protection and Biodiversity Conservation Act 1999, permit WT2005-8619, and under the Antarctic Marine Living Resources Conservation Act 1981, permit AMLR 05-06_2655.

Funding Information:
This work was supported by the Australian Antarctic Division Science Programme [project 4037], [project 4050]; Australian Research Council [grant FS110200057]; United Kingdom Engineering and Physical Sciences Research Council [grant EP/M000869/1].

Publisher Copyright:
© 2015 Taylor & Francis.

All Science Journal Classification (ASJC) codes

Oceanography
Physiology
Aquatic Science

Access to Document

10.1080/10236244.2015.1073455

Cite this

@article{281e5e5bed0e47178494f2fc1a1cab52,

title = "Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques",

abstract = "The accurate observation of physiological changes on in vivo samples of important animal species such as Euphausia superba (Antarctic krill) is an important goal in helping to understand how environmental changes can affect animal development. Using a custom made {\textquoteleft}krill trap{\textquoteright}, live un-anaesthetized krill were confined for seven hours, during which three hours of optical imaging were obtained and no subsequent ill effects observed. The trap enabled two imaging methods to be employed: optical coherence tomography (OCT) and microscopy. OCT enabled internal structure and tissues to be imaged to a depth of approximately 2 mm and resolution of approximately 12 μm. Microscopy was used to observe heart rate. During our experiments, we imaged a range of internal structures in live animals including the heart and gastric areas. The trap design enables a new generation of mixed modality imaging of these animals in vivo. These techniques will enable detailed studies of the internal physiology of live krill to be undertaken under a wide range of environmental conditions and have the potential to highlight important variations in behaviour and animal development.",

author = "Martin Cox and So Kawaguchi and Robert King and Kishan Dholakia and Brown, {Christian T.A.}",

note = "Funding Information: The collection and rearing of krill specimens was funded by the Australian Antarctic Division Science Programme Project 4037 (Experimental Krill Biology: Response of krill to environmental change), and Project 4050 (Assessing change in krill distribution and abundance in Eastern Antarctica). MJC is funded by an Australian Research Council grant FS110200057. CTAB acknowledges support for instrument development and shipping costs from the United Kingdom Engineering and Physical Sciences Research Council Grant EP/M000869/1 (Shaped Light at the Interface). Live wild krill were caught in the Southern Ocean under permits issued by the Australian Department of Environment and Heritage under the Environment Protection and Biodiversity Conservation Act 1999, permit WT2005-8619, and under the Antarctic Marine Living Resources Conservation Act 1981, permit AMLR 05-06_2655. Funding Information: This work was supported by the Australian Antarctic Division Science Programme [project 4037], [project 4050]; Australian Research Council [grant FS110200057]; United Kingdom Engineering and Physical Sciences Research Council [grant EP/M000869/1]. Publisher Copyright: {\textcopyright} 2015 Taylor & Francis.",

year = "2015",

month = nov,

day = "2",

doi = "10.1080/10236244.2015.1073455",

language = "English",

volume = "48",

pages = "455--466",

journal = "Marine and Freshwater Behaviour and Physiology",

issn = "1023-6244",

publisher = "Taylor and Francis Ltd.",

number = "6",

}

TY - JOUR

T1 - Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques

AU - Cox, Martin

AU - Kawaguchi, So

AU - King, Robert

AU - Dholakia, Kishan

AU - Brown, Christian T.A.

N1 - Funding Information: The collection and rearing of krill specimens was funded by the Australian Antarctic Division Science Programme Project 4037 (Experimental Krill Biology: Response of krill to environmental change), and Project 4050 (Assessing change in krill distribution and abundance in Eastern Antarctica). MJC is funded by an Australian Research Council grant FS110200057. CTAB acknowledges support for instrument development and shipping costs from the United Kingdom Engineering and Physical Sciences Research Council Grant EP/M000869/1 (Shaped Light at the Interface). Live wild krill were caught in the Southern Ocean under permits issued by the Australian Department of Environment and Heritage under the Environment Protection and Biodiversity Conservation Act 1999, permit WT2005-8619, and under the Antarctic Marine Living Resources Conservation Act 1981, permit AMLR 05-06_2655. Funding Information: This work was supported by the Australian Antarctic Division Science Programme [project 4037], [project 4050]; Australian Research Council [grant FS110200057]; United Kingdom Engineering and Physical Sciences Research Council [grant EP/M000869/1]. Publisher Copyright: © 2015 Taylor & Francis.

PY - 2015/11/2

Y1 - 2015/11/2

N2 - The accurate observation of physiological changes on in vivo samples of important animal species such as Euphausia superba (Antarctic krill) is an important goal in helping to understand how environmental changes can affect animal development. Using a custom made ‘krill trap’, live un-anaesthetized krill were confined for seven hours, during which three hours of optical imaging were obtained and no subsequent ill effects observed. The trap enabled two imaging methods to be employed: optical coherence tomography (OCT) and microscopy. OCT enabled internal structure and tissues to be imaged to a depth of approximately 2 mm and resolution of approximately 12 μm. Microscopy was used to observe heart rate. During our experiments, we imaged a range of internal structures in live animals including the heart and gastric areas. The trap design enables a new generation of mixed modality imaging of these animals in vivo. These techniques will enable detailed studies of the internal physiology of live krill to be undertaken under a wide range of environmental conditions and have the potential to highlight important variations in behaviour and animal development.

AB - The accurate observation of physiological changes on in vivo samples of important animal species such as Euphausia superba (Antarctic krill) is an important goal in helping to understand how environmental changes can affect animal development. Using a custom made ‘krill trap’, live un-anaesthetized krill were confined for seven hours, during which three hours of optical imaging were obtained and no subsequent ill effects observed. The trap enabled two imaging methods to be employed: optical coherence tomography (OCT) and microscopy. OCT enabled internal structure and tissues to be imaged to a depth of approximately 2 mm and resolution of approximately 12 μm. Microscopy was used to observe heart rate. During our experiments, we imaged a range of internal structures in live animals including the heart and gastric areas. The trap design enables a new generation of mixed modality imaging of these animals in vivo. These techniques will enable detailed studies of the internal physiology of live krill to be undertaken under a wide range of environmental conditions and have the potential to highlight important variations in behaviour and animal development.

UR - http://www.scopus.com/inward/record.url?scp=84945458265&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84945458265&partnerID=8YFLogxK

U2 - 10.1080/10236244.2015.1073455

DO - 10.1080/10236244.2015.1073455

M3 - Article

AN - SCOPUS:84945458265

SN - 1023-6244

VL - 48

SP - 455

EP - 466

JO - Marine and Freshwater Behaviour and Physiology

JF - Marine and Freshwater Behaviour and Physiology

IS - 6

ER -

Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this