Interaction of β-Giardin with the Bop1 Protein in Giardia lamblia

J. Kim; S. Y. Goo; H. J. Chung; H. W. Yang; T. S. Yong; K. H. Lee; S. J. Park

doi:10.1007/s00436-005-0040-8

Interaction of β-Giardin with the Bop1 Protein in Giardia lamblia

J. Kim, S. Y. Goo, H. J. Chung, H. W. Yang, T. S. Yong, K. H. Lee, S. J. Park

Department of Environmental Medical Biology

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

A cDNA clone encoding a putative Bop1 homologous protein was identified in Giardia lamblia. Since Bop1 is a nucleolar protein involved in rRNA processing, thereby controlling the cell cycle, we investigated components of cell cycle control in G. lamblia by identifying the protein(s) that interact with Bop 1. Through an immunoaffinity column made with polyclonal antibodies specific to the recombinant Bop1 of G. lamblia, a pool of proteins was obtained from the crude extracts of Giardia and then used as antigens to immunize rats. By employing the resultant sera for cDNA library immunoscreening, we isolated cDNA clones encoding an immunopurified protein, which turned out to contain the gene for β-giardin, a Giardia-specific cytoskeletal protein. The interaction between Bop1 and β-giardin was confirmed via two different methods, yeast two-hybrid assay and coimmunoprecipitation.

Original language	English
Pages (from-to)	138-144
Number of pages	7
Journal	Parasitology Research
Volume	98
Issue number	2
DOIs	https://doi.org/10.1007/s00436-005-0040-8
Publication status	Published - 2006 Jan

Bibliographical note

Funding Information:
Acknowledgement This work was supported by an Anti-Communicable Diseases Program of the National Institute of Health (NIH 348-6111-215), Ministry of Health and Welfare, Korea.

All Science Journal Classification (ASJC) codes

Parasitology
veterinary(all)
Insect Science
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s00436-005-0040-8

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title = "Interaction of β-Giardin with the Bop1 Protein in Giardia lamblia",

abstract = "A cDNA clone encoding a putative Bop1 homologous protein was identified in Giardia lamblia. Since Bop1 is a nucleolar protein involved in rRNA processing, thereby controlling the cell cycle, we investigated components of cell cycle control in G. lamblia by identifying the protein(s) that interact with Bop 1. Through an immunoaffinity column made with polyclonal antibodies specific to the recombinant Bop1 of G. lamblia, a pool of proteins was obtained from the crude extracts of Giardia and then used as antigens to immunize rats. By employing the resultant sera for cDNA library immunoscreening, we isolated cDNA clones encoding an immunopurified protein, which turned out to contain the gene for β-giardin, a Giardia-specific cytoskeletal protein. The interaction between Bop1 and β-giardin was confirmed via two different methods, yeast two-hybrid assay and coimmunoprecipitation.",

author = "J. Kim and Goo, {S. Y.} and Chung, {H. J.} and Yang, {H. W.} and Yong, {T. S.} and Lee, {K. H.} and Park, {S. J.}",

note = "Funding Information: Acknowledgement This work was supported by an Anti-Communicable Diseases Program of the National Institute of Health (NIH 348-6111-215), Ministry of Health and Welfare, Korea.",

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AU - Kim, J.

AU - Goo, S. Y.

AU - Chung, H. J.

AU - Yang, H. W.

AU - Yong, T. S.

AU - Lee, K. H.

AU - Park, S. J.

N1 - Funding Information: Acknowledgement This work was supported by an Anti-Communicable Diseases Program of the National Institute of Health (NIH 348-6111-215), Ministry of Health and Welfare, Korea.

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N2 - A cDNA clone encoding a putative Bop1 homologous protein was identified in Giardia lamblia. Since Bop1 is a nucleolar protein involved in rRNA processing, thereby controlling the cell cycle, we investigated components of cell cycle control in G. lamblia by identifying the protein(s) that interact with Bop 1. Through an immunoaffinity column made with polyclonal antibodies specific to the recombinant Bop1 of G. lamblia, a pool of proteins was obtained from the crude extracts of Giardia and then used as antigens to immunize rats. By employing the resultant sera for cDNA library immunoscreening, we isolated cDNA clones encoding an immunopurified protein, which turned out to contain the gene for β-giardin, a Giardia-specific cytoskeletal protein. The interaction between Bop1 and β-giardin was confirmed via two different methods, yeast two-hybrid assay and coimmunoprecipitation.

AB - A cDNA clone encoding a putative Bop1 homologous protein was identified in Giardia lamblia. Since Bop1 is a nucleolar protein involved in rRNA processing, thereby controlling the cell cycle, we investigated components of cell cycle control in G. lamblia by identifying the protein(s) that interact with Bop 1. Through an immunoaffinity column made with polyclonal antibodies specific to the recombinant Bop1 of G. lamblia, a pool of proteins was obtained from the crude extracts of Giardia and then used as antigens to immunize rats. By employing the resultant sera for cDNA library immunoscreening, we isolated cDNA clones encoding an immunopurified protein, which turned out to contain the gene for β-giardin, a Giardia-specific cytoskeletal protein. The interaction between Bop1 and β-giardin was confirmed via two different methods, yeast two-hybrid assay and coimmunoprecipitation.

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Interaction of β-Giardin with the Bop1 Protein in Giardia lamblia

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

UN SDGs

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