Integrated single- and two-photon light sheet microscopy using accelerating beams

Peeter Piksarv; Dominik Marti; Tuan Le; Angelika Unterhuber; Lindsey H. Forbes; Melissa R. Andrews; Andreas Stingl; Wolfgang Drexler; Peter E. Andersen; Kishan Dholakia

doi:10.1038/s41598-017-01543-4

Integrated single- and two-photon light sheet microscopy using accelerating beams

Peeter Piksarv, Dominik Marti, Tuan Le, Angelika Unterhuber, Lindsey H. Forbes, Melissa R. Andrews, Andreas Stingl, Wolfgang Drexler, Peter E. Andersen, Kishan Dholakia

Department of Physics

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

We demonstrate the first light sheet microscope using propagation invariant, accelerating Airy beams that operates both in single- and two-photon modes. The use of the Airy beam permits us to develop an ultra compact, high resolution light sheet system without beam scanning. In two-photon mode, an increase in the field of view over the use of a standard Gaussian beam by a factor of six is demonstrated. This implementation for light sheet microscopy opens up new possibilities across a wide range of biomedical applications, especially for the study of neuronal processes.

Original language	English
Article number	1435
Journal	Scientific reports
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/s41598-017-01543-4
Publication status	Published - 2017 Dec 1

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© 2017 The Author(s).

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abstract = "We demonstrate the first light sheet microscope using propagation invariant, accelerating Airy beams that operates both in single- and two-photon modes. The use of the Airy beam permits us to develop an ultra compact, high resolution light sheet system without beam scanning. In two-photon mode, an increase in the field of view over the use of a standard Gaussian beam by a factor of six is demonstrated. This implementation for light sheet microscopy opens up new possibilities across a wide range of biomedical applications, especially for the study of neuronal processes.",

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AU - Le, Tuan

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AU - Forbes, Lindsey H.

AU - Andrews, Melissa R.

AU - Stingl, Andreas

AU - Drexler, Wolfgang

AU - Andersen, Peter E.

AU - Dholakia, Kishan

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Integrated single- and two-photon light sheet microscopy using accelerating beams

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