Integrated 3D macro-trapping and light-sheet imaging system

Zhengyi Yang; Peeter Piksarv; David E.K. Ferrier; Frank J. Gunn-Moore; Kishan Dholakia

doi:10.1117/12.2186399

Integrated 3D macro-trapping and light-sheet imaging system

Zhengyi Yang, Peeter Piksarv, David E.K. Ferrier, Frank J. Gunn-Moore, Kishan Dholakia

Department of Physics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Biological research requires high-speed and low-damage imaging techniques for live specimens in areas such as development study in embryos. Light sheet microscopy provides fast imaging speed whilst keeps the photo-damage and photo-blenching to minimum. Conventional sample embedding methods in light sheet imaging involves using agent such as agarose which potentially affects the behavior and the develop pattern of the specimens. Here we demonstrate integrating dual-beam trapping method into light sheet imaging system to confine and translate the specimen whilst light sheet images are taken. Tobacco plant cells as well as Spirobranchus lamarcki larva were trapped solely with optical force and sectional images were acquired. This now approach has the potential to extend the applications of light sheet imaging significantly.

Original language	English
Title of host publication	Optical Trapping and Optical Micromanipulation XII
Editors	Gabriel C. Spalding, Kishan Dholakia, Kishan Dholakia, Gabriel C. Spalding
Publisher	SPIE
ISBN (Electronic)	9781628417142, 9781628417142
DOIs	https://doi.org/10.1117/12.2186399
Publication status	Published - 2015
Event	Optical Trapping and Optical Micromanipulation XII - San Diego, United States Duration: 2015 Aug 9 → 2015 Aug 12

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	9548
ISSN (Print)	0277-786X
ISSN (Electronic)	1996-756X

Other

Other	Optical Trapping and Optical Micromanipulation XII
Country/Territory	United States
City	San Diego
Period	15/8/9 → 15/8/12

Bibliographical note

Publisher Copyright:
© 2015 SPIE.

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Access to Document

10.1117/12.2186399

Cite this

Yang, Z., Piksarv, P., Ferrier, D. E. K., Gunn-Moore, F. J., & Dholakia, K. (2015). Integrated 3D macro-trapping and light-sheet imaging system. In G. C. Spalding, K. Dholakia, K. Dholakia, & G. C. Spalding (Eds.), Optical Trapping and Optical Micromanipulation XII Article 95480T (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 9548). SPIE. https://doi.org/10.1117/12.2186399

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abstract = "Biological research requires high-speed and low-damage imaging techniques for live specimens in areas such as development study in embryos. Light sheet microscopy provides fast imaging speed whilst keeps the photo-damage and photo-blenching to minimum. Conventional sample embedding methods in light sheet imaging involves using agent such as agarose which potentially affects the behavior and the develop pattern of the specimens. Here we demonstrate integrating dual-beam trapping method into light sheet imaging system to confine and translate the specimen whilst light sheet images are taken. Tobacco plant cells as well as Spirobranchus lamarcki larva were trapped solely with optical force and sectional images were acquired. This now approach has the potential to extend the applications of light sheet imaging significantly.",

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author = "Zhengyi Yang and Peeter Piksarv and Ferrier, {David E.K.} and Gunn-Moore, {Frank J.} and Kishan Dholakia",

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Yang, Z, Piksarv, P, Ferrier, DEK, Gunn-Moore, FJ & Dholakia, K 2015, Integrated 3D macro-trapping and light-sheet imaging system. in GC Spalding, K Dholakia, K Dholakia & GC Spalding (eds), Optical Trapping and Optical Micromanipulation XII., 95480T, Proceedings of SPIE - The International Society for Optical Engineering, vol. 9548, SPIE, Optical Trapping and Optical Micromanipulation XII, San Diego, United States, 15/8/9. https://doi.org/10.1117/12.2186399

Integrated 3D macro-trapping and light-sheet imaging system. / Yang, Zhengyi; Piksarv, Peeter; Ferrier, David E.K. et al.
Optical Trapping and Optical Micromanipulation XII. ed. / Gabriel C. Spalding; Kishan Dholakia; Kishan Dholakia; Gabriel C. Spalding. SPIE, 2015. 95480T (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 9548).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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AB - Biological research requires high-speed and low-damage imaging techniques for live specimens in areas such as development study in embryos. Light sheet microscopy provides fast imaging speed whilst keeps the photo-damage and photo-blenching to minimum. Conventional sample embedding methods in light sheet imaging involves using agent such as agarose which potentially affects the behavior and the develop pattern of the specimens. Here we demonstrate integrating dual-beam trapping method into light sheet imaging system to confine and translate the specimen whilst light sheet images are taken. Tobacco plant cells as well as Spirobranchus lamarcki larva were trapped solely with optical force and sectional images were acquired. This now approach has the potential to extend the applications of light sheet imaging significantly.

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Integrated 3D macro-trapping and light-sheet imaging system

Abstract

Publication series

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Bibliographical note

All Science Journal Classification (ASJC) codes

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