Abstract
Biological research requires high-speed and low-damage imaging techniques for live specimens in areas such as development study in embryos. Light sheet microscopy provides fast imaging speed whilst keeps the photo-damage and photo-blenching to minimum. Conventional sample embedding methods in light sheet imaging involves using agent such as agarose which potentially affects the behavior and the develop pattern of the specimens. Here we demonstrate integrating dual-beam trapping method into light sheet imaging system to confine and translate the specimen whilst light sheet images are taken. Tobacco plant cells as well as Spirobranchus lamarcki larva were trapped solely with optical force and sectional images were acquired. This now approach has the potential to extend the applications of light sheet imaging significantly.
Original language | English |
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Title of host publication | Optical Trapping and Optical Micromanipulation XII |
Editors | Gabriel C. Spalding, Kishan Dholakia, Kishan Dholakia, Gabriel C. Spalding |
Publisher | SPIE |
ISBN (Electronic) | 9781628417142, 9781628417142 |
DOIs | |
Publication status | Published - 2015 |
Event | Optical Trapping and Optical Micromanipulation XII - San Diego, United States Duration: 2015 Aug 9 → 2015 Aug 12 |
Publication series
Name | Proceedings of SPIE - The International Society for Optical Engineering |
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Volume | 9548 |
ISSN (Print) | 0277-786X |
ISSN (Electronic) | 1996-756X |
Other
Other | Optical Trapping and Optical Micromanipulation XII |
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Country/Territory | United States |
City | San Diego |
Period | 15/8/9 → 15/8/12 |
Bibliographical note
Publisher Copyright:© 2015 SPIE.
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Computer Science Applications
- Applied Mathematics
- Electrical and Electronic Engineering