Inhibition of skin inflammation and atopic dermatitis by topical application of a novel ceramide derivative, K112PC-5, in mice

Jong Soon Kang; Chang Woo Lee; Kiho Lee; Mi Hwa Han; Hyunju Lee; Jong Kyung Youm; Se Kyoo Jeong; Byeong Deog Park; Sang Bae Han; Gyoonhee Han; Song Kyu Park; Hwan Mook Kim

doi:10.1007/s12272-001-1260-z

Inhibition of skin inflammation and atopic dermatitis by topical application of a novel ceramide derivative, K112PC-5, in mice

Jong Soon Kang, Chang Woo Lee, Kiho Lee, Mi Hwa Han, Hyunju Lee, Jong Kyung Youm, Se Kyoo Jeong, Byeong Deog Park, Sang Bae Han, Gyoonhee Han, Song Kyu Park, Hwan Mook Kim

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7 Citations (Scopus)

Abstract

PC-9S (N-Ethanol-2-mirystyl-3-oxo-stearamide) is a synthetic ceramide and has been known to be effective in atopic and psoriatic patients. K112PC-5 (2-Acetyl-N-(1,3-dihydroxyisopropyl)-tetradecanamide) is a novel ceramide derivative of PC-9S. In the present study, we examined the effect of K112PC-5 on macrophage and T lymphocyte function in primary macrophages and splenocytes, respectively, as well as the effect of topical application of K112PC-5 on skin inflammation and atopic dermatitis (AD) in mouse models. K112PC-5 inhibited lipopolysaccharide-induced nitrite generation in mouse peritoneal macrophages in a dose-dependent manner. However, K112PC-5 did not affect concanavalin A-induced proliferation, interleukin (IL)-2 secretion and IL-4 secretion in mouse splenocytes. In addition, K112PC-5 significantly suppressed the increase in phorbol ester-induced ear thickness in BALB/c mice. Further study demonstrated that topical application of K112PC-5 also inhibited AD induced by extracts of dust mites, Dermatophagoides pteronyssinus and Dermatophagoides farinae, in NC/Nga mice. Taken together, these results showed that K112PC-5 exerted an anti-inflammatory effect both in vitro and in vivo and proved to be beneficial in an animal model of AD. Our results suggest that K112PC-5 might be beneficial as a topical agent for the treatment of AD.

Original language	English
Pages (from-to)	1004-1009
Number of pages	6
Journal	Archives of pharmacal research
Volume	31
Issue number	8
DOIs	https://doi.org/10.1007/s12272-001-1260-z
Publication status	Published - 2008 Aug

Bibliographical note

Funding Information:
This work was supported by a grant of Components and Materials Technology Development program (10014690), which is managed by Korea Ministry of Commerce, Industry and Energy and a grant from KRIBB Research Initiative Program.

All Science Journal Classification (ASJC) codes

Molecular Medicine
Drug Discovery
Organic Chemistry

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10.1007/s12272-001-1260-z

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@article{493a95a7724843d2a94210b6137ce40a,

title = "Inhibition of skin inflammation and atopic dermatitis by topical application of a novel ceramide derivative, K112PC-5, in mice",

abstract = "PC-9S (N-Ethanol-2-mirystyl-3-oxo-stearamide) is a synthetic ceramide and has been known to be effective in atopic and psoriatic patients. K112PC-5 (2-Acetyl-N-(1,3-dihydroxyisopropyl)-tetradecanamide) is a novel ceramide derivative of PC-9S. In the present study, we examined the effect of K112PC-5 on macrophage and T lymphocyte function in primary macrophages and splenocytes, respectively, as well as the effect of topical application of K112PC-5 on skin inflammation and atopic dermatitis (AD) in mouse models. K112PC-5 inhibited lipopolysaccharide-induced nitrite generation in mouse peritoneal macrophages in a dose-dependent manner. However, K112PC-5 did not affect concanavalin A-induced proliferation, interleukin (IL)-2 secretion and IL-4 secretion in mouse splenocytes. In addition, K112PC-5 significantly suppressed the increase in phorbol ester-induced ear thickness in BALB/c mice. Further study demonstrated that topical application of K112PC-5 also inhibited AD induced by extracts of dust mites, Dermatophagoides pteronyssinus and Dermatophagoides farinae, in NC/Nga mice. Taken together, these results showed that K112PC-5 exerted an anti-inflammatory effect both in vitro and in vivo and proved to be beneficial in an animal model of AD. Our results suggest that K112PC-5 might be beneficial as a topical agent for the treatment of AD.",

author = "Kang, {Jong Soon} and Lee, {Chang Woo} and Kiho Lee and Han, {Mi Hwa} and Hyunju Lee and Youm, {Jong Kyung} and Jeong, {Se Kyoo} and Park, {Byeong Deog} and Han, {Sang Bae} and Gyoonhee Han and Park, {Song Kyu} and Kim, {Hwan Mook}",

note = "Funding Information: This work was supported by a grant of Components and Materials Technology Development program (10014690), which is managed by Korea Ministry of Commerce, Industry and Energy and a grant from KRIBB Research Initiative Program.",

year = "2008",

month = aug,

doi = "10.1007/s12272-001-1260-z",

language = "English",

volume = "31",

pages = "1004--1009",

journal = "Archives of Pharmacal Research",

issn = "0253-6269",

publisher = "Pharmaceutical Society of Korea",

number = "8",

}

TY - JOUR

T1 - Inhibition of skin inflammation and atopic dermatitis by topical application of a novel ceramide derivative, K112PC-5, in mice

AU - Kang, Jong Soon

AU - Lee, Chang Woo

AU - Lee, Kiho

AU - Han, Mi Hwa

AU - Lee, Hyunju

AU - Youm, Jong Kyung

AU - Jeong, Se Kyoo

AU - Park, Byeong Deog

AU - Han, Sang Bae

AU - Han, Gyoonhee

AU - Park, Song Kyu

AU - Kim, Hwan Mook

N1 - Funding Information: This work was supported by a grant of Components and Materials Technology Development program (10014690), which is managed by Korea Ministry of Commerce, Industry and Energy and a grant from KRIBB Research Initiative Program.

PY - 2008/8

Y1 - 2008/8

N2 - PC-9S (N-Ethanol-2-mirystyl-3-oxo-stearamide) is a synthetic ceramide and has been known to be effective in atopic and psoriatic patients. K112PC-5 (2-Acetyl-N-(1,3-dihydroxyisopropyl)-tetradecanamide) is a novel ceramide derivative of PC-9S. In the present study, we examined the effect of K112PC-5 on macrophage and T lymphocyte function in primary macrophages and splenocytes, respectively, as well as the effect of topical application of K112PC-5 on skin inflammation and atopic dermatitis (AD) in mouse models. K112PC-5 inhibited lipopolysaccharide-induced nitrite generation in mouse peritoneal macrophages in a dose-dependent manner. However, K112PC-5 did not affect concanavalin A-induced proliferation, interleukin (IL)-2 secretion and IL-4 secretion in mouse splenocytes. In addition, K112PC-5 significantly suppressed the increase in phorbol ester-induced ear thickness in BALB/c mice. Further study demonstrated that topical application of K112PC-5 also inhibited AD induced by extracts of dust mites, Dermatophagoides pteronyssinus and Dermatophagoides farinae, in NC/Nga mice. Taken together, these results showed that K112PC-5 exerted an anti-inflammatory effect both in vitro and in vivo and proved to be beneficial in an animal model of AD. Our results suggest that K112PC-5 might be beneficial as a topical agent for the treatment of AD.

AB - PC-9S (N-Ethanol-2-mirystyl-3-oxo-stearamide) is a synthetic ceramide and has been known to be effective in atopic and psoriatic patients. K112PC-5 (2-Acetyl-N-(1,3-dihydroxyisopropyl)-tetradecanamide) is a novel ceramide derivative of PC-9S. In the present study, we examined the effect of K112PC-5 on macrophage and T lymphocyte function in primary macrophages and splenocytes, respectively, as well as the effect of topical application of K112PC-5 on skin inflammation and atopic dermatitis (AD) in mouse models. K112PC-5 inhibited lipopolysaccharide-induced nitrite generation in mouse peritoneal macrophages in a dose-dependent manner. However, K112PC-5 did not affect concanavalin A-induced proliferation, interleukin (IL)-2 secretion and IL-4 secretion in mouse splenocytes. In addition, K112PC-5 significantly suppressed the increase in phorbol ester-induced ear thickness in BALB/c mice. Further study demonstrated that topical application of K112PC-5 also inhibited AD induced by extracts of dust mites, Dermatophagoides pteronyssinus and Dermatophagoides farinae, in NC/Nga mice. Taken together, these results showed that K112PC-5 exerted an anti-inflammatory effect both in vitro and in vivo and proved to be beneficial in an animal model of AD. Our results suggest that K112PC-5 might be beneficial as a topical agent for the treatment of AD.

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U2 - 10.1007/s12272-001-1260-z

DO - 10.1007/s12272-001-1260-z

M3 - Article

C2 - 18787789

AN - SCOPUS:51849152423

SN - 0253-6269

VL - 31

SP - 1004

EP - 1009

JO - Archives of Pharmacal Research

JF - Archives of Pharmacal Research

IS - 8

ER -

Inhibition of skin inflammation and atopic dermatitis by topical application of a novel ceramide derivative, K112PC-5, in mice

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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