TY - GEN
T1 - Inhibition of apoptosis by taurine in macrophages treated with sodium nitroprusside
AU - Kim, So Young
AU - Park, Taesun
AU - Kim, Ha Won
PY - 2009
Y1 - 2009
N2 - Nitric oxide (NO) induces apoptotic cell death in murine RAW264.7 macrophages. To elucidate the mechanism underlying the inhibitory effect of taurine on NO-induced apoptosis, a cell was exposed to sodium nitroprusside (SNP), an NO donor, in the absence and presence of taurine. Taurine treatment prevented SNP-mediated cellular apoptosis in a concentration dependent manner. The exposure of the cell to taurine prior to SNP treatment inhibited DNA fragmentation more than addition of taurine to the medium after SNP treatment. Agarose gel electrophoresis data revealed that taurine reduced the intensity of SNP-induced DNA laddering. The taurine-mediated reduction in the number of apoptotic cells was also observed using the Hoechst 33258 stain. These results support the idea that taurine has the potential to function as an inhibitory modulator of NO-mediated cell injury.
AB - Nitric oxide (NO) induces apoptotic cell death in murine RAW264.7 macrophages. To elucidate the mechanism underlying the inhibitory effect of taurine on NO-induced apoptosis, a cell was exposed to sodium nitroprusside (SNP), an NO donor, in the absence and presence of taurine. Taurine treatment prevented SNP-mediated cellular apoptosis in a concentration dependent manner. The exposure of the cell to taurine prior to SNP treatment inhibited DNA fragmentation more than addition of taurine to the medium after SNP treatment. Agarose gel electrophoresis data revealed that taurine reduced the intensity of SNP-induced DNA laddering. The taurine-mediated reduction in the number of apoptotic cells was also observed using the Hoechst 33258 stain. These results support the idea that taurine has the potential to function as an inhibitory modulator of NO-mediated cell injury.
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U2 - 10.1007/978-0-387-75681-3_50
DO - 10.1007/978-0-387-75681-3_50
M3 - Conference contribution
C2 - 19239180
AN - SCOPUS:63149177310
SN - 9780387756806
T3 - Advances in Experimental Medicine and Biology
SP - 481
EP - 489
BT - Taurine 7
A2 - Azuma, Junichi
A2 - Takashi, Ito
A2 - Schaffer, Stephen
ER -