Influence of reactive species on the modification of biomolecules generated from the soft plasma

Pankaj Attri; Naresh Kumar; Ji Hoon Park; Dharmendra Kumar Yadav; Sooho Choi; Han S. Uhm; In Tae Kim; Eun Ha Choi; Weontae Lee

doi:10.1038/srep08221

Influence of reactive species on the modification of biomolecules generated from the soft plasma

Pankaj Attri, Naresh Kumar, Ji Hoon Park, Dharmendra Kumar Yadav, Sooho Choi, Han S. Uhm, In Tae Kim, Eun Ha Choi, Weontae Lee

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Abstract

Plasma medicine is an upcoming research area that has attracted the scientists to explore more deeply the utility of plasma. So, apart from the treating biomaterials and tissues with plasma, we have studied the effect of soft plasma with different feeding gases such as Air, N₂ and Ar on modification of biomolecules. Hence, in this work we have used the soft plasma on biomolecules such as proteins ((Hemoglobin (Hb) and Myoglobin (Mb)), calf thymus DNA and amino acids. The structural changes or structural modification of proteins and DNA have been studied using circular dichroism (CD), fluorescence spectroscopy, protein oxidation test, gel electrophoresis, UV-vis spectroscopy, dynamic light scattering (DLS) and 1D NMR, while Liquid Chromatograph/Capillary Electrophoresis-Mass Spectrometer (LC/CE-MS) based on qualitative and quantitative bio-analysis have been used to study the modification of amino acids. Further, the thermal analysis of the protein has been studied with differential scanning calorimetry (DSC) and CD. Additionally, we have performed docking studies of H₂O₂ with Hb and Mb, which reveals that H₂O₂ molecules preferably attack the amino acids near heme group. We have also shown that N₂ gas plasma has strong deformation action on biomolecules and compared to other gases plasma.

Original language	English
Article number	8221
Journal	Scientific reports
Volume	5
DOIs	https://doi.org/10.1038/srep08221
Publication status	Published - 2015 Feb 4

Bibliographical note

Funding Information:
We gratefully acknowledge SRC program of National Research Foundation of Korea (NRF) Grant funded by the Korean Government (MEST) (No. 2010-0027963) and in part by Kwangwoon University 2014. This work was also supported by Mid-career Researcher Program (NRF-2013R1A2A2A01068963) through NRF grant funded by the MEST and in part by the Brain Korea 21(BK21) PLUS program (Sooho Choi, Yonsei U.).

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10.1038/srep08221

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title = "Influence of reactive species on the modification of biomolecules generated from the soft plasma",

abstract = "Plasma medicine is an upcoming research area that has attracted the scientists to explore more deeply the utility of plasma. So, apart from the treating biomaterials and tissues with plasma, we have studied the effect of soft plasma with different feeding gases such as Air, N2 and Ar on modification of biomolecules. Hence, in this work we have used the soft plasma on biomolecules such as proteins ((Hemoglobin (Hb) and Myoglobin (Mb)), calf thymus DNA and amino acids. The structural changes or structural modification of proteins and DNA have been studied using circular dichroism (CD), fluorescence spectroscopy, protein oxidation test, gel electrophoresis, UV-vis spectroscopy, dynamic light scattering (DLS) and 1D NMR, while Liquid Chromatograph/Capillary Electrophoresis-Mass Spectrometer (LC/CE-MS) based on qualitative and quantitative bio-analysis have been used to study the modification of amino acids. Further, the thermal analysis of the protein has been studied with differential scanning calorimetry (DSC) and CD. Additionally, we have performed docking studies of H2O2 with Hb and Mb, which reveals that H2O2 molecules preferably attack the amino acids near heme group. We have also shown that N2 gas plasma has strong deformation action on biomolecules and compared to other gases plasma.",

author = "Pankaj Attri and Naresh Kumar and Park, {Ji Hoon} and Yadav, {Dharmendra Kumar} and Sooho Choi and Uhm, {Han S.} and Kim, {In Tae} and Choi, {Eun Ha} and Weontae Lee",

note = "Funding Information: We gratefully acknowledge SRC program of National Research Foundation of Korea (NRF) Grant funded by the Korean Government (MEST) (No. 2010-0027963) and in part by Kwangwoon University 2014. This work was also supported by Mid-career Researcher Program (NRF-2013R1A2A2A01068963) through NRF grant funded by the MEST and in part by the Brain Korea 21(BK21) PLUS program (Sooho Choi, Yonsei U.).",

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AU - Uhm, Han S.

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N1 - Funding Information: We gratefully acknowledge SRC program of National Research Foundation of Korea (NRF) Grant funded by the Korean Government (MEST) (No. 2010-0027963) and in part by Kwangwoon University 2014. This work was also supported by Mid-career Researcher Program (NRF-2013R1A2A2A01068963) through NRF grant funded by the MEST and in part by the Brain Korea 21(BK21) PLUS program (Sooho Choi, Yonsei U.).

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Influence of reactive species on the modification of biomolecules generated from the soft plasma

Abstract

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