The Mxi1 proteins are biochemical and biological antagonists of c-myc oncoprotein. It has been reported that the overexpression pattern of c-myc might be similar to a molecular feature of early and late stages of human autosomal dominant polycystic kidney disease. We identified the cyst phenotype in Mxi1-deficient mice aged 6-12 months using H&E staining. Some chemokines containing a protein domain similar to human IL-8, which is associated with the inflammatory response, were subsequently selected from the up-regulated genes. We confirmed the expression level of these chemokines and measured protein concentrations of IL-8 using ELISA in the Mxi1-knockdown cells. IL-8 was found to be significantly increased in Mxi1-knockdown cells. We found that p38 MAP kinase activation was involved in the signal transduction of the Mxi1-inactivated secretion of IL-8. Therefore, we could suggest that the inactivation of Mxi1 leads to the inflammatory response and has the potential to induce polycystic renal disease.
|Number of pages
|Biochemical and Biophysical Research Communications
|Published - 2007 Apr 27
Bibliographical noteFunding Information:
This work was supported by the Korea Science and Engineering Foundation (KOSEF) through the National Research Lab. Program funded by the Ministry of Science and Technology (M10500000101-06J0000-10110), KOSEF grant funded by the Korea government (MOST) (ROI-2004-000-10182-0), Grants from 21C Frontier FHGP (FG05-22-02) and the BRC Frontier (M103KV010018-05K2201-01830). We are grateful to Dr. J.Y. Noh at Sookmyng Women’s University for discussions.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology