In vivo study for clinical application of dental stem cell therapy incorporated with dental titanium implants

Hyunmin Choi, Kyu Hyung Park, Narae Jung, June Sung Shim, Hong Seok Moon, Hyung Jun Kim, Seung Han Oh, Yoon Young Kim, Seung Yup Ku, Young Bum Park

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


The aim of this study was to investigate the behavior of dental-derived human mesenchymal stem cells (d-hMSCs) in response to differently surface-treated implants and to evaluate the effect of d-hMSCs on local osteogenesis around an implant in vivo. d-hMSCs derived from alveolar bone were established and cultured on machined, sandblasted and acid-etched (SLA)-treated titanium discs with and without osteogenic induction medium. Their morphological and osteogenic potential was assessed by scanning electron microscopy (SEM) and real-time polymerase chain reaction (RT-PCR) via mixing of 5 × 106 of d-hMSCs with 1 mL of Metrigel and 20 µL of gel-cell mixture, which was dispensed into the defect followed by the placement of customized mini-implants (machined, SLA-treated implants) in New Zealand white rabbits. Following healing periods of 2 weeks and 12 weeks, the obtained samples in each group were analyzed radiographically, histomorphometrically and immunohistochemically. The quantitative change in osteogenic differentiation of d-hMSCs was identified according to the type of surface treatment. Radiographic analysis revealed that an increase in new bone formation was statistically significant in the d-hMSCs group. Histomorphometric analysis was in accordance with radiographic analysis, showing the significantly increased new bone formation in the d-hMSCs group regardless of time of sacrifice. Human nuclei A was identified near the area where d-hMSCs were implanted but the level of expression was found to be decreased as time passed. Within the limitations of the present study, in this animal model, the transplantation of d-hMSCs enhanced the new bone formation around an implant and the survival and function of the stem cells was experimentally proven up to 12 weeks post-sacrifice.

Original languageEnglish
Article number381
Pages (from-to)1-19
Number of pages19
Issue number2
Publication statusPublished - 2021 Jan 2

Bibliographical note

Funding Information:
Funding: This research was supported by the Brain Korea 21 plus project, Yonsei University College of Dentistry, and the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2016R1D1A1B03934584, 2020R1F1A1049978).

Publisher Copyright:
© 2021 by the authors. Li-censee MDPI, Basel, Switzerland.

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics


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