Abstract
It is known that oestrogen influences skin wound healing by modulating the inflammatory response, cytokine expression and extracellular matrix deposition; accelerating re-epithelialization; and stimulating angiogenesis. To identify novel proteins associated with effects of oestrogen on keratinocyte, stable isotope labelling by amino acids in cell culture (SILAC)-based mass spectrometry was performed. Using SILAC, quantification of 1085 proteins was achieved. Among these proteins, 60 proteins were upregulated and 32 proteins were downregulated. Among significantly upregulated proteins, high-mobility group protein B1 (HMGB1) has been further evaluated for its role in the effect of oestrogen on keratinocytes. HMGB1 expression was strongly induced in oestrogen-treated keratinocytes in dose- and time-dependent manner. Further, HMGB1 was able to significantly accelerate the rate of HaCaT cell migration. To determine whether HMGB1 is involved in E2-induced HaCaT cell migration, cells were transfected with HMGB1 siRNA. Knockdown of HMGB1 blocked oestrogen-induced keratinocyte migration. Collectively, these experiments demonstrate that HMGB1 is a novel downstream mediator of oestrogen-stimulated keratinocyte migration.
| Original language | English |
|---|---|
| Pages (from-to) | 478-480 |
| Number of pages | 3 |
| Journal | Experimental dermatology |
| Volume | 24 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2015 Jun 1 |
Bibliographical note
Publisher Copyright:© 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Biology
- Dermatology