In vivo O2 measurement inside single photosynthetic cells

Seoung Jai Bai; Won Hyoung Ryu; Rainer J. Fasching; Arthur R. Grossman; Fritz B. Prinz

doi:10.1007/s10529-011-0604-x

In vivo O₂ measurement inside single photosynthetic cells

Seoung Jai Bai, Won Hyoung Ryu, Rainer J. Fasching, Arthur R. Grossman, Fritz B. Prinz

Department of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

The oxygen evolution of single cells was investigated using a nano-probe with an ultra-micro electrode (UME) in a submicron sized system in combination with a micro-fluidic system. A single cell was immobilized in the micro-fluidic system and a nano-probe was inserted into the cytosolic space of the single cell. Then, the UME was used for an in vivo amperometric experiment at a fixed potential and electrochemical impedance spectroscopy to detect oxygen evolution of the single cell under various light intensities.

Original language	English
Pages (from-to)	1675-1681
Number of pages	7
Journal	Biotechnology Letters
Volume	33
Issue number	8
DOIs	https://doi.org/10.1007/s10529-011-0604-x
Publication status	Published - 2011 Aug

Bibliographical note

Funding Information:
Acknowledgment This work was supported by the Global Climate and Energy Project at Stanford University. S. Bai acknowledges additional support by Ministry of Environment under Environment Convergence Technology Project.

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

Access to Document

10.1007/s10529-011-0604-x

Cite this

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abstract = "The oxygen evolution of single cells was investigated using a nano-probe with an ultra-micro electrode (UME) in a submicron sized system in combination with a micro-fluidic system. A single cell was immobilized in the micro-fluidic system and a nano-probe was inserted into the cytosolic space of the single cell. Then, the UME was used for an in vivo amperometric experiment at a fixed potential and electrochemical impedance spectroscopy to detect oxygen evolution of the single cell under various light intensities.",

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AU - Fasching, Rainer J.

AU - Grossman, Arthur R.

AU - Prinz, Fritz B.

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