In vivo optical modulation of neural signals using monolithically integrated two-dimensional neural probe arrays

Yoojin Son, Hyunjoo Jenny Lee, Jeongyeon Kim, Hyogeun Shin, Nakwon Choi, C. Justin Lee, Eui Sung Yoon, Euisik Yoon, Kensall D. Wise, Tae Geun Kim, Il Joo Cho

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72 Citations (Scopus)


Integration of stimulation modalities (e.g. electrical, optical, and chemical) on a large array of neural probes can enable an investigation of important underlying mechanisms of brain disorders that is not possible through neural recordings alone. Furthermore, it is important to achieve this integration of multiple functionalities in a compact structure to utilize a large number of the mouse models. Here we present a successful optical modulation of in vivo neural signals of a transgenic mouse through our compact 2D MEMS neural array (optrodes). Using a novel fabrication method that embeds a lower cladding layer in a silicon substrate, we achieved a thin silicon 2D optrode array that is capable of delivering light to multiple sites using SU-8 as a waveguide core. Without additional modification to the microelectrodes, the measured impedance of the multiple microelectrodes was below 1 MΩ at 1 kHz. In addition, with a low background noise level (±25μV), neural spikes from different individual neurons were recorded on each microelectrode. Lastly, we successfully used our optrodes to modulate the neural activity of a transgenic mouse through optical stimulation. These results demonstrate the functionality of the 2D optrode array and its potential as a next-generation tool for optogenetic applications.

Original languageEnglish
Article number15466
JournalScientific reports
Publication statusPublished - 2015 Oct 23

Bibliographical note

Funding Information:
This work was partially supported by the KIST Institutional Program (2E24184), the Technology Innovation Program (10053023, The Development of RF MEMS Devices Core Technology for Multiband IoT System Applications) funded By the Ministry of Trade, industry & Energy (MI, Korea) and National Research Foundation of Korea (NRF) grant funded by the Korean government (No. 2011-0028769).

All Science Journal Classification (ASJC) codes

  • General


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