In situ lysis droplet supply to efficiently extract ATP from dust particles for near-real-time bioaerosol monitoring

Hyeong Rae Kim; Sanggwon An; Jungho Hwang; Jae Hong Park; Jeong Hoon Byeon

doi:10.1016/j.jhazmat.2019.02.088

In situ lysis droplet supply to efficiently extract ATP from dust particles for near-real-time bioaerosol monitoring

Hyeong Rae Kim, Sanggwon An, Jungho Hwang, Jae Hong Park, Jeong Hoon Byeon

Department of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Simultaneous improvement in detection speed and reliability is critical for bioaerosol monitoring. Recent rapid detection strategies exhibit difficulties with misinterpretation due to signal interference from co-existing nonbiological particles, whereas biomolecular and bioluminescent approaches require long process times (>several tens of minutes) to generate readable values despite their better detection reliability. To overcome these shortcomings, we designed a system to achieve rapid reliable field detection of bioaerosols (>10⁴ relative luminescence units [RLU] per cubic meter of air) in <3 min processing time (equivalent to 24 L sampling air volume) by employing a lysis droplet supply for efficient extraction of adenosine triphosphate (ATP) from particulate matter (PM) and a photomultiplier tube detector for signal amplification of ATP bioluminescence. We also suggested the use of the ratio of RLU (m⁻³) to total PM (μg m⁻³), or specific bioluminescence (RLU μg⁻¹), as a measure of the biofraction of PM (i.e., potential biohazards). A correlation between RLU and colony forming unit was also obtained from simultaneous aerosol sampling using an agar-inserted sampler.

Original language	English
Pages (from-to)	684-690
Number of pages	7
Journal	Journal of Hazardous Materials
Volume	369
DOIs	https://doi.org/10.1016/j.jhazmat.2019.02.088
Publication status	Published - 2019 May 5

Bibliographical note

Funding Information:
This work was supported from Bio Nano Health-Guard Research Center funded by the Ministry of Science, ICT & Future Planning (MSIP) of Korea as Global Frontier Project (Grant Number H-GUARD_2013M3A6B2078959 ). We also acknowledge the efforts of the members of Prof. Jeong Hoon Byeon’s Mechanical Engineering Project 2 (course 1393 in 2018) for the field tests and system preparation. Appendix A

Publisher Copyright:
© 2019 Elsevier B.V.

All Science Journal Classification (ASJC) codes

Environmental Engineering
Environmental Chemistry
Waste Management and Disposal
Pollution
Health, Toxicology and Mutagenesis

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10.1016/j.jhazmat.2019.02.088

Cite this

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title = "In situ lysis droplet supply to efficiently extract ATP from dust particles for near-real-time bioaerosol monitoring",

abstract = "Simultaneous improvement in detection speed and reliability is critical for bioaerosol monitoring. Recent rapid detection strategies exhibit difficulties with misinterpretation due to signal interference from co-existing nonbiological particles, whereas biomolecular and bioluminescent approaches require long process times (>several tens of minutes) to generate readable values despite their better detection reliability. To overcome these shortcomings, we designed a system to achieve rapid reliable field detection of bioaerosols (>104 relative luminescence units [RLU] per cubic meter of air) in <3 min processing time (equivalent to 24 L sampling air volume) by employing a lysis droplet supply for efficient extraction of adenosine triphosphate (ATP) from particulate matter (PM) and a photomultiplier tube detector for signal amplification of ATP bioluminescence. We also suggested the use of the ratio of RLU (m−3) to total PM (μg m−3), or specific bioluminescence (RLU μg−1), as a measure of the biofraction of PM (i.e., potential biohazards). A correlation between RLU and colony forming unit was also obtained from simultaneous aerosol sampling using an agar-inserted sampler.",

author = "Kim, {Hyeong Rae} and Sanggwon An and Jungho Hwang and Park, {Jae Hong} and Byeon, {Jeong Hoon}",

note = "Funding Information: This work was supported from Bio Nano Health-Guard Research Center funded by the Ministry of Science, ICT & Future Planning (MSIP) of Korea as Global Frontier Project (Grant Number H-GUARD_2013M3A6B2078959 ). We also acknowledge the efforts of the members of Prof. Jeong Hoon Byeon{\textquoteright}s Mechanical Engineering Project 2 (course 1393 in 2018) for the field tests and system preparation. Appendix A Publisher Copyright: {\textcopyright} 2019 Elsevier B.V.",

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N2 - Simultaneous improvement in detection speed and reliability is critical for bioaerosol monitoring. Recent rapid detection strategies exhibit difficulties with misinterpretation due to signal interference from co-existing nonbiological particles, whereas biomolecular and bioluminescent approaches require long process times (>several tens of minutes) to generate readable values despite their better detection reliability. To overcome these shortcomings, we designed a system to achieve rapid reliable field detection of bioaerosols (>104 relative luminescence units [RLU] per cubic meter of air) in <3 min processing time (equivalent to 24 L sampling air volume) by employing a lysis droplet supply for efficient extraction of adenosine triphosphate (ATP) from particulate matter (PM) and a photomultiplier tube detector for signal amplification of ATP bioluminescence. We also suggested the use of the ratio of RLU (m−3) to total PM (μg m−3), or specific bioluminescence (RLU μg−1), as a measure of the biofraction of PM (i.e., potential biohazards). A correlation between RLU and colony forming unit was also obtained from simultaneous aerosol sampling using an agar-inserted sampler.

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In situ lysis droplet supply to efficiently extract ATP from dust particles for near-real-time bioaerosol monitoring

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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