Immunoscintigraphy in the detection of tuberculosis with radiolabelled antibody fragment against Mycobacterium bovis bacillus Calmette-Guérin: a preliminary study in a rabbit model

Jong Doo Lee; Kyu Ho Shin; Sang Nae Cho; Jun Soo Shin; Min Geol Lee; Woo Ik Yang; Chang Yoon Park; Hyung Sik Yoo; Jong Tae Lee; Ok Doo Awh

doi:10.1007/BF00180861

Immunoscintigraphy in the detection of tuberculosis with radiolabelled antibody fragment against Mycobacterium bovis bacillus Calmette-Guérin: a preliminary study in a rabbit model

Jong Doo Lee, Kyu Ho Shin, Sang Nae Cho, Jun Soo Shin, Min Geol Lee, Woo Ik Yang, Chang Yoon Park, Hyung Sik Yoo, Jong Tae Lee, Ok Doo Awh

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Abstract

Immunoscintigraphy with radiolabelled monoclonal antibodies is widely used to detect solid tumours, but only a few trials have been carried out concerning the specific in vivo localization of an inflammatory process. The purpose of this study was to investigate the detectability of tuberculous foci utilizing this method with radiolabelled bacillus Calmette-Guérin (BCG)-specific F(ab′)₂ in rabbits. All of the tuberculous lesions (n=8) were clearly visualized on serial scintigraphy for up to 48 h after injection of the antibody. Immunohistochemical and Ziel-Neelson staining of the tuberculous lesions confirmed the presence of the tuberculous antigens and bacilli. It failed to demonstrate any sustained retention of the BCG-specific antibody fragment in the control group with syphilitic orchitis (n=2). Therefore, the specific in vivo localization of tuberculosis is feasible by immunoscintigraphy.

Original language	English
Pages (from-to)	1011-1015
Number of pages	5
Journal	European Journal of Nuclear Medicine
Volume	19
Issue number	12
DOIs	https://doi.org/10.1007/BF00180861
Publication status	Published - 1992 Dec

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Radiology Nuclear Medicine and imaging

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10.1007/BF00180861

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Lee, J. D., Shin, K. H., Cho, S. N., Shin, J. S., Lee, M. G., Yang, W. I., Park, C. Y., Yoo, H. S., Lee, J. T., & Awh, O. D. (1992). Immunoscintigraphy in the detection of tuberculosis with radiolabelled antibody fragment against Mycobacterium bovis bacillus Calmette-Guérin: a preliminary study in a rabbit model. European Journal of Nuclear Medicine, 19(12), 1011-1015. https://doi.org/10.1007/BF00180861

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title = "Immunoscintigraphy in the detection of tuberculosis with radiolabelled antibody fragment against Mycobacterium bovis bacillus Calmette-Gu{\'e}rin: a preliminary study in a rabbit model",

abstract = "Immunoscintigraphy with radiolabelled monoclonal antibodies is widely used to detect solid tumours, but only a few trials have been carried out concerning the specific in vivo localization of an inflammatory process. The purpose of this study was to investigate the detectability of tuberculous foci utilizing this method with radiolabelled bacillus Calmette-Gu{\'e}rin (BCG)-specific F(ab′)2 in rabbits. All of the tuberculous lesions (n=8) were clearly visualized on serial scintigraphy for up to 48 h after injection of the antibody. Immunohistochemical and Ziel-Neelson staining of the tuberculous lesions confirmed the presence of the tuberculous antigens and bacilli. It failed to demonstrate any sustained retention of the BCG-specific antibody fragment in the control group with syphilitic orchitis (n=2). Therefore, the specific in vivo localization of tuberculosis is feasible by immunoscintigraphy.",

author = "Lee, {Jong Doo} and Shin, {Kyu Ho} and Cho, {Sang Nae} and Shin, {Jun Soo} and Lee, {Min Geol} and Yang, {Woo Ik} and Park, {Chang Yoon} and Yoo, {Hyung Sik} and Lee, {Jong Tae} and Awh, {Ok Doo}",

year = "1992",

month = dec,

doi = "10.1007/BF00180861",

language = "English",

volume = "19",

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}

Lee, JD, Shin, KH, Cho, SN, Shin, JS, Lee, MG, Yang, WI, Park, CY, Yoo, HS, Lee, JT & Awh, OD 1992, 'Immunoscintigraphy in the detection of tuberculosis with radiolabelled antibody fragment against Mycobacterium bovis bacillus Calmette-Guérin: a preliminary study in a rabbit model', European Journal of Nuclear Medicine, vol. 19, no. 12, pp. 1011-1015. https://doi.org/10.1007/BF00180861

Immunoscintigraphy in the detection of tuberculosis with radiolabelled antibody fragment against Mycobacterium bovis bacillus Calmette-Guérin: a preliminary study in a rabbit model. / Lee, Jong Doo; Shin, Kyu Ho; Cho, Sang Nae et al.
In: European Journal of Nuclear Medicine, Vol. 19, No. 12, 12.1992, p. 1011-1015.

Research output: Contribution to journal › Article › peer-review

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AU - Shin, Jun Soo

AU - Lee, Min Geol

AU - Yang, Woo Ik

AU - Park, Chang Yoon

AU - Yoo, Hyung Sik

AU - Lee, Jong Tae

AU - Awh, Ok Doo

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AB - Immunoscintigraphy with radiolabelled monoclonal antibodies is widely used to detect solid tumours, but only a few trials have been carried out concerning the specific in vivo localization of an inflammatory process. The purpose of this study was to investigate the detectability of tuberculous foci utilizing this method with radiolabelled bacillus Calmette-Guérin (BCG)-specific F(ab′)2 in rabbits. All of the tuberculous lesions (n=8) were clearly visualized on serial scintigraphy for up to 48 h after injection of the antibody. Immunohistochemical and Ziel-Neelson staining of the tuberculous lesions confirmed the presence of the tuberculous antigens and bacilli. It failed to demonstrate any sustained retention of the BCG-specific antibody fragment in the control group with syphilitic orchitis (n=2). Therefore, the specific in vivo localization of tuberculosis is feasible by immunoscintigraphy.

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Immunoscintigraphy in the detection of tuberculosis with radiolabelled antibody fragment against Mycobacterium bovis bacillus Calmette-Guérin: a preliminary study in a rabbit model

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

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