Immunological characterization of 130 kDa phospholipase C-β4 isozyme in rat cerebellar Purkinje cells

Do Sik Min; Myung Jong Kim; Hyun Kyung Jeong; Young Han Lee; Hyun Kim; Hee Sup Shin; Sung Ho Ryu; Pann Ghill Suh

doi:10.1016/S0304-3940(00)01419-1

Immunological characterization of 130 kDa phospholipase C-β4 isozyme in rat cerebellar Purkinje cells

Do Sik Min, Myung Jong Kim, Hyun Kyung Jeong, Young Han Lee, Hyun Kim, Hee Sup Shin, Sung Ho Ryu, Pann Ghill Suh

Department of Pharmacy

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

We have developed a monoclonal antibody, β4-22-9 that recognizes an epitope on pleckstrin homology (PH) domain (1-136 amino acids) located at the amino terminus of rat 130 kDa phospholipase C-β4 (PLC-β4). Tissue distribution of 130 kDa PLC-β4 was determined with this monoclonal antibody. One hundred and thirty kiloDalton PLC-β4 was exclusively expressed in the nervous tissues including cerebellum and cerebrum, especially at very high levels in cerebellum. The expression of 130 kDa PLC-β4 in cerebellum was gradually increased from 5 days after birth to adulthood. In the adult cerebellum, especially high level of 130 kDa PLC-β4 was localized on the periphery of Purkinje cells in the patch-like pattern. These data implies that 130 kDa PLC-β4 may a role in cerebellar Purkinje cells. (C) 2000 Elsevier Science Ireland Ltd.

Original language	English
Pages (from-to)	9-12
Number of pages	4
Journal	Neuroscience Letters
Volume	292
Issue number	1
DOIs	https://doi.org/10.1016/S0304-3940(00)01419-1
Publication status	Published - 2000 Sept 29

Bibliographical note

Funding Information:
This work was supported by the Brain Science & Engineering Research Program (98-J04-02-01-A-03) of the Ministry of Science and Technology in Korea. M.J. Kim was supported by a Creative Research Initiative program grant from the Ministry of Science and Technology in Korea.

All Science Journal Classification (ASJC) codes

Neuroscience(all)

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10.1016/S0304-3940(00)01419-1

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title = "Immunological characterization of 130 kDa phospholipase C-β4 isozyme in rat cerebellar Purkinje cells",

abstract = "We have developed a monoclonal antibody, β4-22-9 that recognizes an epitope on pleckstrin homology (PH) domain (1-136 amino acids) located at the amino terminus of rat 130 kDa phospholipase C-β4 (PLC-β4). Tissue distribution of 130 kDa PLC-β4 was determined with this monoclonal antibody. One hundred and thirty kiloDalton PLC-β4 was exclusively expressed in the nervous tissues including cerebellum and cerebrum, especially at very high levels in cerebellum. The expression of 130 kDa PLC-β4 in cerebellum was gradually increased from 5 days after birth to adulthood. In the adult cerebellum, especially high level of 130 kDa PLC-β4 was localized on the periphery of Purkinje cells in the patch-like pattern. These data implies that 130 kDa PLC-β4 may a role in cerebellar Purkinje cells. (C) 2000 Elsevier Science Ireland Ltd.",

author = "Min, {Do Sik} and Kim, {Myung Jong} and Jeong, {Hyun Kyung} and Lee, {Young Han} and Hyun Kim and Shin, {Hee Sup} and Ryu, {Sung Ho} and Suh, {Pann Ghill}",

note = "Funding Information: This work was supported by the Brain Science & Engineering Research Program (98-J04-02-01-A-03) of the Ministry of Science and Technology in Korea. M.J. Kim was supported by a Creative Research Initiative program grant from the Ministry of Science and Technology in Korea.",

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doi = "10.1016/S0304-3940(00)01419-1",

language = "English",

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AU - Min, Do Sik

AU - Kim, Myung Jong

AU - Jeong, Hyun Kyung

AU - Lee, Young Han

AU - Kim, Hyun

AU - Shin, Hee Sup

AU - Ryu, Sung Ho

AU - Suh, Pann Ghill

N1 - Funding Information: This work was supported by the Brain Science & Engineering Research Program (98-J04-02-01-A-03) of the Ministry of Science and Technology in Korea. M.J. Kim was supported by a Creative Research Initiative program grant from the Ministry of Science and Technology in Korea.

PY - 2000/9/29

Y1 - 2000/9/29

N2 - We have developed a monoclonal antibody, β4-22-9 that recognizes an epitope on pleckstrin homology (PH) domain (1-136 amino acids) located at the amino terminus of rat 130 kDa phospholipase C-β4 (PLC-β4). Tissue distribution of 130 kDa PLC-β4 was determined with this monoclonal antibody. One hundred and thirty kiloDalton PLC-β4 was exclusively expressed in the nervous tissues including cerebellum and cerebrum, especially at very high levels in cerebellum. The expression of 130 kDa PLC-β4 in cerebellum was gradually increased from 5 days after birth to adulthood. In the adult cerebellum, especially high level of 130 kDa PLC-β4 was localized on the periphery of Purkinje cells in the patch-like pattern. These data implies that 130 kDa PLC-β4 may a role in cerebellar Purkinje cells. (C) 2000 Elsevier Science Ireland Ltd.

AB - We have developed a monoclonal antibody, β4-22-9 that recognizes an epitope on pleckstrin homology (PH) domain (1-136 amino acids) located at the amino terminus of rat 130 kDa phospholipase C-β4 (PLC-β4). Tissue distribution of 130 kDa PLC-β4 was determined with this monoclonal antibody. One hundred and thirty kiloDalton PLC-β4 was exclusively expressed in the nervous tissues including cerebellum and cerebrum, especially at very high levels in cerebellum. The expression of 130 kDa PLC-β4 in cerebellum was gradually increased from 5 days after birth to adulthood. In the adult cerebellum, especially high level of 130 kDa PLC-β4 was localized on the periphery of Purkinje cells in the patch-like pattern. These data implies that 130 kDa PLC-β4 may a role in cerebellar Purkinje cells. (C) 2000 Elsevier Science Ireland Ltd.

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Immunological characterization of 130 kDa phospholipase C-β4 isozyme in rat cerebellar Purkinje cells

Abstract

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