Immunohistochemical localization of phospholipase D2 in embryonic rat brain

Jeong Sun Choi; Hyun Jung Park; Young Chan Jo; Myung Hoon Chun; Jin Woong Chung; Joon Mo Kim; Do Sik Min; Mun Yong Lee

doi:10.1016/j.neulet.2003.12.054

Immunohistochemical localization of phospholipase D2 in embryonic rat brain

Jeong Sun Choi, Hyun Jung Park, Young Chan Jo, Myung Hoon Chun, Jin Woong Chung, Joon Mo Kim, Do Sik Min, Mun Yong Lee

Department of Pharmacy

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The present study has characterized the cellular and temporal localization of the phospholipase D2 (PLD2) protein in the embryonic rat brain, using immunohistochemistry. PLD2 immunoreactivity was first observed in the choroid plexus and in the most ventricular zone of the lateral and third ventricles at embryonic day 15 (E15), followed by gradual restriction to the limited zone of ventricles at E20. In addition, PLD2 expression was high in the developing cerebral cortex and hippocampus. In the cortex, PLD2 expression was observed in the marginal zone from the earliest stage (E15) and then declined and had completely disappeared by E20. Double-labelling studies demonstrated co-expression of the anti-class III β-tubulin antibody in most of the PLD2 immunoreactive cells. Therefore, our findings suggest that PLD2 may be involved in early developmental processes of some neuronal progenitors.

Original language	English
Pages (from-to)	147-151
Number of pages	5
Journal	Neuroscience Letters
Volume	357
Issue number	2
DOIs	https://doi.org/10.1016/j.neulet.2003.12.054
Publication status	Published - 2004 Mar 4

Bibliographical note

Funding Information:
This work was supported by the Basic Research Program of the Korea Science and Engineering Foundation (Grant Number: 2000-0-213-001-2).

All Science Journal Classification (ASJC) codes

Neuroscience(all)

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10.1016/j.neulet.2003.12.054

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title = "Immunohistochemical localization of phospholipase D2 in embryonic rat brain",

abstract = "The present study has characterized the cellular and temporal localization of the phospholipase D2 (PLD2) protein in the embryonic rat brain, using immunohistochemistry. PLD2 immunoreactivity was first observed in the choroid plexus and in the most ventricular zone of the lateral and third ventricles at embryonic day 15 (E15), followed by gradual restriction to the limited zone of ventricles at E20. In addition, PLD2 expression was high in the developing cerebral cortex and hippocampus. In the cortex, PLD2 expression was observed in the marginal zone from the earliest stage (E15) and then declined and had completely disappeared by E20. Double-labelling studies demonstrated co-expression of the anti-class III β-tubulin antibody in most of the PLD2 immunoreactive cells. Therefore, our findings suggest that PLD2 may be involved in early developmental processes of some neuronal progenitors.",

author = "Choi, {Jeong Sun} and Park, {Hyun Jung} and Jo, {Young Chan} and Chun, {Myung Hoon} and Chung, {Jin Woong} and Kim, {Joon Mo} and Min, {Do Sik} and Lee, {Mun Yong}",

note = "Funding Information: This work was supported by the Basic Research Program of the Korea Science and Engineering Foundation (Grant Number: 2000-0-213-001-2). ",

year = "2004",

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doi = "10.1016/j.neulet.2003.12.054",

language = "English",

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T1 - Immunohistochemical localization of phospholipase D2 in embryonic rat brain

AU - Choi, Jeong Sun

AU - Park, Hyun Jung

AU - Jo, Young Chan

AU - Chun, Myung Hoon

AU - Chung, Jin Woong

AU - Kim, Joon Mo

AU - Min, Do Sik

AU - Lee, Mun Yong

N1 - Funding Information: This work was supported by the Basic Research Program of the Korea Science and Engineering Foundation (Grant Number: 2000-0-213-001-2).

PY - 2004/3/4

Y1 - 2004/3/4

N2 - The present study has characterized the cellular and temporal localization of the phospholipase D2 (PLD2) protein in the embryonic rat brain, using immunohistochemistry. PLD2 immunoreactivity was first observed in the choroid plexus and in the most ventricular zone of the lateral and third ventricles at embryonic day 15 (E15), followed by gradual restriction to the limited zone of ventricles at E20. In addition, PLD2 expression was high in the developing cerebral cortex and hippocampus. In the cortex, PLD2 expression was observed in the marginal zone from the earliest stage (E15) and then declined and had completely disappeared by E20. Double-labelling studies demonstrated co-expression of the anti-class III β-tubulin antibody in most of the PLD2 immunoreactive cells. Therefore, our findings suggest that PLD2 may be involved in early developmental processes of some neuronal progenitors.

AB - The present study has characterized the cellular and temporal localization of the phospholipase D2 (PLD2) protein in the embryonic rat brain, using immunohistochemistry. PLD2 immunoreactivity was first observed in the choroid plexus and in the most ventricular zone of the lateral and third ventricles at embryonic day 15 (E15), followed by gradual restriction to the limited zone of ventricles at E20. In addition, PLD2 expression was high in the developing cerebral cortex and hippocampus. In the cortex, PLD2 expression was observed in the marginal zone from the earliest stage (E15) and then declined and had completely disappeared by E20. Double-labelling studies demonstrated co-expression of the anti-class III β-tubulin antibody in most of the PLD2 immunoreactive cells. Therefore, our findings suggest that PLD2 may be involved in early developmental processes of some neuronal progenitors.

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Immunohistochemical localization of phospholipase D2 in embryonic rat brain

Abstract

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