Immunodiagnosis of clonorchiasis using a recombinant antigen.

T. S. Yong; H. J. Yang; S. J. Park; Y. K. Kim; D. H. Lee; S. M. Lee

doi:10.3347/kjp.1998.36.3.183

Immunodiagnosis of clonorchiasis using a recombinant antigen.

T. S. Yong, H. J. Yang, S. J. Park, Y. K. Kim, D. H. Lee, S. M. Lee

Department of Environmental Medical Biology

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

A cDNA expression library of Clonorchis sinensis adult worm was constructed, and screened out immunologically. One clone, pBCs31, was selected in view of its predominant reactivity with an experimentally infected rabbit serum. Recombinant C. sinensis antigen with 28 kDa as a beta-galactosidase fusion protein produced in Escherichia coli was identified by immunoblot analysis. The cloned gene was composed of 16 copies of a 30 base pair repeat and an additional 320 bases. The deduced amino acid sequence of the tandem repeat was AQPPKSGDGG. On RNA slot blot analysis. C. sinensis adult worm RNA showed a positive reaction with the cloned gene. Enzyme-linked immunosorbent assay using a purified recombinant antigen of pBCs31 showed high specificity for diagnosis of clonorchiasis.

Original language	English
Pages (from-to)	183-190
Number of pages	8
Journal	The Korean journal of parasitology
Volume	36
Issue number	3
DOIs	https://doi.org/10.3347/kjp.1998.36.3.183
Publication status	Published - 1998

All Science Journal Classification (ASJC) codes

Parasitology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3347/kjp.1998.36.3.183

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title = "Immunodiagnosis of clonorchiasis using a recombinant antigen.",

abstract = "A cDNA expression library of Clonorchis sinensis adult worm was constructed, and screened out immunologically. One clone, pBCs31, was selected in view of its predominant reactivity with an experimentally infected rabbit serum. Recombinant C. sinensis antigen with 28 kDa as a beta-galactosidase fusion protein produced in Escherichia coli was identified by immunoblot analysis. The cloned gene was composed of 16 copies of a 30 base pair repeat and an additional 320 bases. The deduced amino acid sequence of the tandem repeat was AQPPKSGDGG. On RNA slot blot analysis. C. sinensis adult worm RNA showed a positive reaction with the cloned gene. Enzyme-linked immunosorbent assay using a purified recombinant antigen of pBCs31 showed high specificity for diagnosis of clonorchiasis.",

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AU - Yong, T. S.

AU - Yang, H. J.

AU - Park, S. J.

AU - Kim, Y. K.

AU - Lee, D. H.

AU - Lee, S. M.

PY - 1998

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AB - A cDNA expression library of Clonorchis sinensis adult worm was constructed, and screened out immunologically. One clone, pBCs31, was selected in view of its predominant reactivity with an experimentally infected rabbit serum. Recombinant C. sinensis antigen with 28 kDa as a beta-galactosidase fusion protein produced in Escherichia coli was identified by immunoblot analysis. The cloned gene was composed of 16 copies of a 30 base pair repeat and an additional 320 bases. The deduced amino acid sequence of the tandem repeat was AQPPKSGDGG. On RNA slot blot analysis. C. sinensis adult worm RNA showed a positive reaction with the cloned gene. Enzyme-linked immunosorbent assay using a purified recombinant antigen of pBCs31 showed high specificity for diagnosis of clonorchiasis.

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Immunodiagnosis of clonorchiasis using a recombinant antigen.

Abstract

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