Immortalized gingival fibroblasts as a cytotoxicity test model for dental materials

Rasika P. Illeperuma, Jin Kim, Young J. Park, Jin M. Kim, Jung Y. Bae, Zhong M. Che, Hwa K. Son, Mi R. Han, Kwang M. Kim

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47 Citations (Scopus)


In vitro cytotoxicity test is an initial step to identify the harmful effects of new dental materials. Aim of this study was to develop a stable human cell line derived from normal gingival fibroblasts (hNOF) and to assess its feasibility in in vitro cytotoxicity testing. Immortalized human gingival fibroblasts (hTERT-hNOF) were successfully established with human telomerase reverse transcriptase gene transfection, preserving its phenotypical characteristics, replicative potential and biological properties. Utilizing standard cytotoxicity test modeling and dental materials, hTERT-hNOF were evaluated for their feasibility in cytotoxicity testing, compared with hNOF and L929 cells. Similar pattern of cytotoxic response was observed among hNOF, hTERT-hNOF and L929 cells. Cytotoxicity response of hTERT-hNOF was significantly similar to hNOF, moreover hTERT-hNOF and hNOF were found to be more sensitive towards the tested dental materials compared to L929 cells. This study suggested that hTERT-hNOF is an effective cytotoxic test model for dental materials.

Original languageEnglish
Pages (from-to)753-762
Number of pages10
JournalJournal of Materials Science: Materials in Medicine
Issue number3
Publication statusPublished - 2012 Mar

Bibliographical note

Funding Information:
Acknowledgments This study was supported by the Priority Research Centers Program through the National Research Foundation of South Korea (NRF) funded by the Ministry of Education, Science and Technology (2009-0094028) and the NRF (R13-2003-013-04001-0). The authors also would like to extend sincere thanks to Professor Darren Williams, Professor Da-Woon Jung and N. Tyagi of Department of Life Science, Gwangju Institute of Science and Technology, Korea, for critical reading of the manuscript and Professor Ki Yeol Kim of the Oral Cancer Research Institute, Yonsei University College of Dentistry, for the support in statistical data analysis.

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering


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