Immobilization of lipase on surface modified magnetic nanoparticles using alkyl benzenesulfonate

Kanagasabai Muruganandam Ponvel; Dong Geun Lee; Eun Ji Woo; Ik Sung Ahn; Chang Ha Lee

doi:10.1007/s11814-009-0019-8

Immobilization of lipase on surface modified magnetic nanoparticles using alkyl benzenesulfonate

Kanagasabai Muruganandam Ponvel, Dong Geun Lee, Eun Ji Woo, Ik Sung Ahn, Chang Ha Lee

Department of Chemical and Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

The surface of nano-sized magnetite (NSM) particles synthesized by coprecipitation method was modified by alkyl benzenesulfonate (ABS) as a coating material. ABS on the NSM was expected to form a spacer between the surface of the NSM particles and the enzyme adsorbed and to play a role of strong enzyme adsorption onto a hydrophobic surface. Transmission electron microscopy showed that the NSM particles had an average size of 10 nm. Magnetic measurement revealed that the nanoparticles were superparamagnetic and the saturation magnetization was about 68 emu/g. Porcine pancreas lipase (PPL) was immobilized onto the ABS-NSM, which was to catalyze hydrolysis of olive oil and showed enhanced durability in the reuse after being recovered by magnetic separations.

Original language	English
Pages (from-to)	127-130
Number of pages	4
Journal	Korean Journal of Chemical Engineering
Volume	26
Issue number	1
DOIs	https://doi.org/10.1007/s11814-009-0019-8
Publication status	Published - 2009 Jan

Bibliographical note

Funding Information:
This work was supported by Korea Research Foundation Grant (KRF-2005-005-J01402).

All Science Journal Classification (ASJC) codes

Chemistry(all)
Chemical Engineering(all)

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10.1007/s11814-009-0019-8

Cite this

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title = "Immobilization of lipase on surface modified magnetic nanoparticles using alkyl benzenesulfonate",

abstract = "The surface of nano-sized magnetite (NSM) particles synthesized by coprecipitation method was modified by alkyl benzenesulfonate (ABS) as a coating material. ABS on the NSM was expected to form a spacer between the surface of the NSM particles and the enzyme adsorbed and to play a role of strong enzyme adsorption onto a hydrophobic surface. Transmission electron microscopy showed that the NSM particles had an average size of 10 nm. Magnetic measurement revealed that the nanoparticles were superparamagnetic and the saturation magnetization was about 68 emu/g. Porcine pancreas lipase (PPL) was immobilized onto the ABS-NSM, which was to catalyze hydrolysis of olive oil and showed enhanced durability in the reuse after being recovered by magnetic separations.",

author = "Ponvel, {Kanagasabai Muruganandam} and Lee, {Dong Geun} and Woo, {Eun Ji} and Ahn, {Ik Sung} and Lee, {Chang Ha}",

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AU - Ponvel, Kanagasabai Muruganandam

AU - Lee, Dong Geun

AU - Woo, Eun Ji

AU - Ahn, Ik Sung

AU - Lee, Chang Ha

N1 - Funding Information: This work was supported by Korea Research Foundation Grant (KRF-2005-005-J01402).

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AB - The surface of nano-sized magnetite (NSM) particles synthesized by coprecipitation method was modified by alkyl benzenesulfonate (ABS) as a coating material. ABS on the NSM was expected to form a spacer between the surface of the NSM particles and the enzyme adsorbed and to play a role of strong enzyme adsorption onto a hydrophobic surface. Transmission electron microscopy showed that the NSM particles had an average size of 10 nm. Magnetic measurement revealed that the nanoparticles were superparamagnetic and the saturation magnetization was about 68 emu/g. Porcine pancreas lipase (PPL) was immobilized onto the ABS-NSM, which was to catalyze hydrolysis of olive oil and showed enhanced durability in the reuse after being recovered by magnetic separations.

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Immobilization of lipase on surface modified magnetic nanoparticles using alkyl benzenesulfonate

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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