TY - JOUR
T1 - IL-13 suppresses MUC5AC gene expression and mucin secretion in nasal epithelial cells
AU - Kim, C. H.
AU - Seob Song, K.
AU - Koo, J. S.
AU - Kim, H. U.
AU - Choi, J. Y.
AU - Kim, H. J.
AU - Yoon, J. H.
PY - 2002
Y1 - 2002
N2 - Recently, it was found that IL-13 is involved in allergic inflammation and mucus hypersecretion in in vivo animal models. However, the role of IL-13 in in vitro cellular models has yet to be determined. This study aimed to investigate the direct effect of IL-13 on mucin gene expression and mucin secretion in cultured normal human nasal epithelial cells. After treatment with IL-13, total mucin and MUC5AC mucin levels were measured using an immunoblotting assay. MUC2, MUC5AC and MUC8 mRNA expression were determined using reverse transcription-polymerase chain reaction. Also, immunostaining was performed using a MUC5AC antibody on histologic and cytospin slides. After treatment with ≥ 5 ng/ml IL-13, the level of total mucin and MUC5AC mucin secretion decreased substantially. The expression of MUC2 and MUC8 mRNA increased with higher concentrations of IL-13, but the expression of MUC5AC mRNA decreased. On the 7th day after IL-13 treatment, a significant decrease in the number of MUC5AC-positive cells was confirmed by immunostaining. These findings indicate that IL-13 suppresses MUC5AC mucin gene expression and mucin secretion in cultured normal human nasal epithelial cells in vitro.
AB - Recently, it was found that IL-13 is involved in allergic inflammation and mucus hypersecretion in in vivo animal models. However, the role of IL-13 in in vitro cellular models has yet to be determined. This study aimed to investigate the direct effect of IL-13 on mucin gene expression and mucin secretion in cultured normal human nasal epithelial cells. After treatment with IL-13, total mucin and MUC5AC mucin levels were measured using an immunoblotting assay. MUC2, MUC5AC and MUC8 mRNA expression were determined using reverse transcription-polymerase chain reaction. Also, immunostaining was performed using a MUC5AC antibody on histologic and cytospin slides. After treatment with ≥ 5 ng/ml IL-13, the level of total mucin and MUC5AC mucin secretion decreased substantially. The expression of MUC2 and MUC8 mRNA increased with higher concentrations of IL-13, but the expression of MUC5AC mRNA decreased. On the 7th day after IL-13 treatment, a significant decrease in the number of MUC5AC-positive cells was confirmed by immunostaining. These findings indicate that IL-13 suppresses MUC5AC mucin gene expression and mucin secretion in cultured normal human nasal epithelial cells in vitro.
UR - http://www.scopus.com/inward/record.url?scp=0036395206&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036395206&partnerID=8YFLogxK
U2 - 10.1080/000164802320396321
DO - 10.1080/000164802320396321
M3 - Article
C2 - 12403127
AN - SCOPUS:0036395206
SN - 0001-6489
VL - 122
SP - 638
EP - 643
JO - Acta Oto-Laryngologica
JF - Acta Oto-Laryngologica
IS - 6
ER -