Identification of PCR product by non-surface-binding method using label-free field-effect transistor

Jungim Han; Jeoyoung Shim; Wonseok Chung; Kyutae Yoo; Soosuk Lee; Euisik Yoon; Christopher Ko

Identification of PCR product by non-surface-binding method using label-free field-effect transistor

Jungim Han, Jeoyoung Shim, Wonseok Chung, Kyutae Yoo, Soosuk Lee, Euisik Yoon, Christopher Ko

Yonsei Advanced Science Institute

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We demonstrate an innovative method of PCR product identification that is based on thermal denaturation of PCR product-captured bead using integrated BioFET. Laborious purification and concentration of PCR product were easily carried by capturing the samples on the solid surface like microbeads. When temperature raised upto the denaturation point of PCR product, potential of BioFET is changed by mobile DNA in solution from PCR product-captured beads. By differentiating potential change with respect to temperature change, melting temperature can be determined. In this way, we successfully discriminated three kinds of PCR product using their different melting temperature and improved sensitivity of detection. We expect that proposed method can potentially be used for the identification of multiplex PCR products.

Original language	English
Title of host publication	Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008
Pages	632-635
Number of pages	4
Publication status	Published - 2008
Event	2008 NSTI Nanotechnology Conference and Trade Show, NSTI Nanotech 2008 Joint Meeting, Nanotechnology 2008 - Quebec City, QC, United States Duration: 2008 Jun 1 → 2008 Jun 5

Publication series

Name	Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008
Volume	2

Other

Other	2008 NSTI Nanotechnology Conference and Trade Show, NSTI Nanotech 2008 Joint Meeting, Nanotechnology 2008
Country/Territory	United States
City	Quebec City, QC
Period	08/6/1 → 08/6/5

All Science Journal Classification (ASJC) codes

Mechanical Engineering

Cite this

Han, J., Shim, J., Chung, W., Yoo, K., Lee, S., Yoon, E., & Ko, C. (2008). Identification of PCR product by non-surface-binding method using label-free field-effect transistor. In Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008 (pp. 632-635). (Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008; Vol. 2).

Han, Jungim ; Shim, Jeoyoung ; Chung, Wonseok et al. / Identification of PCR product by non-surface-binding method using label-free field-effect transistor. Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008. 2008. pp. 632-635 (Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008).

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title = "Identification of PCR product by non-surface-binding method using label-free field-effect transistor",

abstract = "We demonstrate an innovative method of PCR product identification that is based on thermal denaturation of PCR product-captured bead using integrated BioFET. Laborious purification and concentration of PCR product were easily carried by capturing the samples on the solid surface like microbeads. When temperature raised upto the denaturation point of PCR product, potential of BioFET is changed by mobile DNA in solution from PCR product-captured beads. By differentiating potential change with respect to temperature change, melting temperature can be determined. In this way, we successfully discriminated three kinds of PCR product using their different melting temperature and improved sensitivity of detection. We expect that proposed method can potentially be used for the identification of multiplex PCR products.",

author = "Jungim Han and Jeoyoung Shim and Wonseok Chung and Kyutae Yoo and Soosuk Lee and Euisik Yoon and Christopher Ko",

year = "2008",

language = "English",

isbn = "9781420085075",

series = "Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008",

pages = "632--635",

booktitle = "Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008",

note = "2008 NSTI Nanotechnology Conference and Trade Show, NSTI Nanotech 2008 Joint Meeting, Nanotechnology 2008 ; Conference date: 01-06-2008 Through 05-06-2008",

}

Han, J, Shim, J, Chung, W, Yoo, K, Lee, S, Yoon, E & Ko, C 2008, Identification of PCR product by non-surface-binding method using label-free field-effect transistor. in Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008. Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008, vol. 2, pp. 632-635, 2008 NSTI Nanotechnology Conference and Trade Show, NSTI Nanotech 2008 Joint Meeting, Nanotechnology 2008, Quebec City, QC, United States, 08/6/1.

Identification of PCR product by non-surface-binding method using label-free field-effect transistor. / Han, Jungim; Shim, Jeoyoung; Chung, Wonseok et al.
Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008. 2008. p. 632-635 (Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008; Vol. 2).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Identification of PCR product by non-surface-binding method using label-free field-effect transistor

AU - Han, Jungim

AU - Shim, Jeoyoung

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AU - Lee, Soosuk

AU - Yoon, Euisik

AU - Ko, Christopher

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AB - We demonstrate an innovative method of PCR product identification that is based on thermal denaturation of PCR product-captured bead using integrated BioFET. Laborious purification and concentration of PCR product were easily carried by capturing the samples on the solid surface like microbeads. When temperature raised upto the denaturation point of PCR product, potential of BioFET is changed by mobile DNA in solution from PCR product-captured beads. By differentiating potential change with respect to temperature change, melting temperature can be determined. In this way, we successfully discriminated three kinds of PCR product using their different melting temperature and improved sensitivity of detection. We expect that proposed method can potentially be used for the identification of multiplex PCR products.

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M3 - Conference contribution

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BT - Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008

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ER -

Han J, Shim J, Chung W, Yoo K, Lee S, Yoon E et al. Identification of PCR product by non-surface-binding method using label-free field-effect transistor. In Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008. 2008. p. 632-635. (Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, NSTI-Nanotech, Nanotechnology 2008).

Identification of PCR product by non-surface-binding method using label-free field-effect transistor

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All Science Journal Classification (ASJC) codes

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