Identification of Mycobacterium species in FFPE granulomatous lymphadenitis tissue using REBA Myco-ID®

J. Munkhdelger, H. Y. Wang, Y. Choi, P. M. Wairagu, D. Lee, S. Park, S. Kim, B. Y. Jeon, H. Lee, K. H. Park

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4 Citations (Scopus)


SETTING: Tuberculous lymphadenitis is the most common form of lymphadenopathy; its main histopathological finding is granulomatous inflammation. OBJECTIVE: A reverse blot hybridisation assay, REBA Myco-ID®, was applied to formalin-fixed paraffin-embedded (FFPE) tissue showing granulomatous lymphadenitis to define the causative agents. DESIGN: A total of 119 granulomatous lymphadenitis cases observed between 2000 and 2010 were studied. All tissue samples were treated by haematoxylin and eosin and Ziehl-Neelsen stain. Mycobacterium tuberculosis and non-tuberculous mycobacteria (NTM) were identified using the REBA Myco-ID assay, and resistance to rifampicin (RMP) and isoniazid (INH) was determined using REBA MTB-MDR®. RESULTS: Of the 119 cases, 113 (95%) were positive with the REBA Myco-ID assay, while 20 (16.8%) were positive on acid-fast bacilli smear. Of the 113 positive REBA Myco-ID cases, 110 (92.43%) were identified as M. tuberculosis, 2 (1.7%) as NTM, and 1 (0.8%) as coinfection with M. tuberculosis and M. chelonae. Only 1 (0.9%) of the 110 M. tuberculosis cases was identified as RMP-resistant. CONCLUSION: REBA Myco-ID is a highly sensitive and specific assay for detecting M. tuberculosis and NTM. M. tuberculosis is the main cause of granulomatous lymphadenitis.

Original languageEnglish
Pages (from-to)898-902
Number of pages5
JournalInternational Journal of Tuberculosis and Lung Disease
Issue number7
Publication statusPublished - 2013 Jul 1

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine
  • Infectious Diseases


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