Identification of EBP50 as a specific biomarker for carcinogens via the analysis of mouse lymphoma cellular proteome

Yoen Jung Lee; In Kwon Choi; Yhun Yhong Sheen; Sue Nie Park; Ho Jeong Kwon

doi:10.1007/s10059-012-2280-7

Identification of EBP50 as a specific biomarker for carcinogens via the analysis of mouse lymphoma cellular proteome

Yoen Jung Lee, In Kwon Choi, Yhun Yhong Sheen, Sue Nie Park, Ho Jeong Kwon

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

To identify specific biomarkers generated upon exposure of L5178Y mouse lymphoma cells to carcinogens, 2-DE and MALDI-TOF MS analysis were conducted using the cellular proteome of L5178Y cells that had been treated with the known carcinogens, 1,2-dibromoethane and O-nitrotoluene and the noncarcinogens, emodin and D-mannitol. Eight protein spots that showed a greater than 1.5-fold increase or decrease in intensity following carcinogen treatment compared with treatment with noncarcinogens were selected. Of the identified proteins, we focused on the candidate biomarker ERM-binding phosphoprotein 50(EBP50), the expression of which was specifically increased in response to treatment with the carcinogens. The expression level of EBP50 was determined by western analysis using polyclonal rabbit anti-EBP50 antibody. Further, the expression level of EBP50 was increased in cells treated with seven additional carcinogens, verifying that EBP50 could serve as a specific biomarker for carcinogens.

Original language	English
Pages (from-to)	309-316
Number of pages	8
Journal	Molecules and cells
Volume	33
Issue number	3
DOIs	https://doi.org/10.1007/s10059-012-2280-7
Publication status	Published - 2012 Mar

Bibliographical note

Funding Information:
This study was partly supported by grants from the Division of Genetic Toxicology National Institute of Toxicological Research, KFDA, the National Research Foundation of Korea funded by the Korean Government (MEST; 2009-0092964, 2010-0017984, F01-2009-000-10183-0), Translational Research Center for Protein Function Control, the Next-Generation BioGreen 21 Program (2011-8-1178), Rural Development Administration, National R&D Program, Ministry of Health & Welfare, and from the Brain Korea 21 Project, Republic of Korea. We thank Drs. Young-ki Paik and Jong Shin Yoo for their valuable comments and Korea Basic Science Institute (KBSI) and Yonsei Proteome Research Center (YPRC) for technical support of proteomic analysis.

All Science Journal Classification (ASJC) codes

Molecular Biology
Cell Biology

Access to Document

10.1007/s10059-012-2280-7

Cite this

@article{5eb54c6b50764486b85866978f9a6b1c,

title = "Identification of EBP50 as a specific biomarker for carcinogens via the analysis of mouse lymphoma cellular proteome",

abstract = "To identify specific biomarkers generated upon exposure of L5178Y mouse lymphoma cells to carcinogens, 2-DE and MALDI-TOF MS analysis were conducted using the cellular proteome of L5178Y cells that had been treated with the known carcinogens, 1,2-dibromoethane and O-nitrotoluene and the noncarcinogens, emodin and D-mannitol. Eight protein spots that showed a greater than 1.5-fold increase or decrease in intensity following carcinogen treatment compared with treatment with noncarcinogens were selected. Of the identified proteins, we focused on the candidate biomarker ERM-binding phosphoprotein 50(EBP50), the expression of which was specifically increased in response to treatment with the carcinogens. The expression level of EBP50 was determined by western analysis using polyclonal rabbit anti-EBP50 antibody. Further, the expression level of EBP50 was increased in cells treated with seven additional carcinogens, verifying that EBP50 could serve as a specific biomarker for carcinogens.",

author = "Lee, {Yoen Jung} and Choi, {In Kwon} and Sheen, {Yhun Yhong} and Park, {Sue Nie} and Kwon, {Ho Jeong}",

note = "Funding Information: This study was partly supported by grants from the Division of Genetic Toxicology National Institute of Toxicological Research, KFDA, the National Research Foundation of Korea funded by the Korean Government (MEST; 2009-0092964, 2010-0017984, F01-2009-000-10183-0), Translational Research Center for Protein Function Control, the Next-Generation BioGreen 21 Program (2011-8-1178), Rural Development Administration, National R&D Program, Ministry of Health & Welfare, and from the Brain Korea 21 Project, Republic of Korea. We thank Drs. Young-ki Paik and Jong Shin Yoo for their valuable comments and Korea Basic Science Institute (KBSI) and Yonsei Proteome Research Center (YPRC) for technical support of proteomic analysis.",

year = "2012",

month = mar,

doi = "10.1007/s10059-012-2280-7",

language = "English",

volume = "33",

pages = "309--316",

journal = "Molecules and cells",

issn = "1016-8478",

publisher = "Korean Society for Molecular and Cellular Biology",

number = "3",

}

TY - JOUR

T1 - Identification of EBP50 as a specific biomarker for carcinogens via the analysis of mouse lymphoma cellular proteome

AU - Lee, Yoen Jung

AU - Choi, In Kwon

AU - Sheen, Yhun Yhong

AU - Park, Sue Nie

AU - Kwon, Ho Jeong

N1 - Funding Information: This study was partly supported by grants from the Division of Genetic Toxicology National Institute of Toxicological Research, KFDA, the National Research Foundation of Korea funded by the Korean Government (MEST; 2009-0092964, 2010-0017984, F01-2009-000-10183-0), Translational Research Center for Protein Function Control, the Next-Generation BioGreen 21 Program (2011-8-1178), Rural Development Administration, National R&D Program, Ministry of Health & Welfare, and from the Brain Korea 21 Project, Republic of Korea. We thank Drs. Young-ki Paik and Jong Shin Yoo for their valuable comments and Korea Basic Science Institute (KBSI) and Yonsei Proteome Research Center (YPRC) for technical support of proteomic analysis.

PY - 2012/3

Y1 - 2012/3

N2 - To identify specific biomarkers generated upon exposure of L5178Y mouse lymphoma cells to carcinogens, 2-DE and MALDI-TOF MS analysis were conducted using the cellular proteome of L5178Y cells that had been treated with the known carcinogens, 1,2-dibromoethane and O-nitrotoluene and the noncarcinogens, emodin and D-mannitol. Eight protein spots that showed a greater than 1.5-fold increase or decrease in intensity following carcinogen treatment compared with treatment with noncarcinogens were selected. Of the identified proteins, we focused on the candidate biomarker ERM-binding phosphoprotein 50(EBP50), the expression of which was specifically increased in response to treatment with the carcinogens. The expression level of EBP50 was determined by western analysis using polyclonal rabbit anti-EBP50 antibody. Further, the expression level of EBP50 was increased in cells treated with seven additional carcinogens, verifying that EBP50 could serve as a specific biomarker for carcinogens.

AB - To identify specific biomarkers generated upon exposure of L5178Y mouse lymphoma cells to carcinogens, 2-DE and MALDI-TOF MS analysis were conducted using the cellular proteome of L5178Y cells that had been treated with the known carcinogens, 1,2-dibromoethane and O-nitrotoluene and the noncarcinogens, emodin and D-mannitol. Eight protein spots that showed a greater than 1.5-fold increase or decrease in intensity following carcinogen treatment compared with treatment with noncarcinogens were selected. Of the identified proteins, we focused on the candidate biomarker ERM-binding phosphoprotein 50(EBP50), the expression of which was specifically increased in response to treatment with the carcinogens. The expression level of EBP50 was determined by western analysis using polyclonal rabbit anti-EBP50 antibody. Further, the expression level of EBP50 was increased in cells treated with seven additional carcinogens, verifying that EBP50 could serve as a specific biomarker for carcinogens.

UR - http://www.scopus.com/inward/record.url?scp=84863449883&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863449883&partnerID=8YFLogxK

U2 - 10.1007/s10059-012-2280-7

DO - 10.1007/s10059-012-2280-7

M3 - Article

C2 - 22434383

AN - SCOPUS:84863449883

SN - 1016-8478

VL - 33

SP - 309

EP - 316

JO - Molecules and cells

JF - Molecules and cells

IS - 3

ER -

Identification of EBP50 as a specific biomarker for carcinogens via the analysis of mouse lymphoma cellular proteome

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this