The cdc25 gene, which is highly conserved in many eukaryotes, encodes a phosphatase that plays essential roles in cell cycle regulation. We identified a cdc25 ortholog in the pinewood nematode, Bursaphelenchus xylophilus. The B. xylophilus ortholog (Bx-cdc25) was found to be highly similar to Caenorhabditis elegans cdc-25.2 in sequence as well as in gene structure, both having long intron 1. The Bx-cdc25 gene was determined to be composed of seven exons and six introns in a 2,580 bp region, and was shown to encode 360 amino acids of a protein containing a highly-conserved phosphatase domain. Bx-cdc25 mRNA was hardly detectable throughout the juvenile stages but was highly expressed in eggs and in both female and male adults. Functional conservation during germline development between C. elegans cdc25 and Bx-cdc25 was revealed by Bx-cdc25 RNA interference in C. elegans.
Bibliographical noteFunding Information:
We thank Bum-Ho Yoo and Hyeon-Sook Koo (Yonsei University) for technical support for soaking RNAi on B. xylophilus. This work was supported by Forest Science and Technology Project (No. S110708L0505604C) from the Korea Forest Service, KRF 2006-005-J03401, and the second Brain Korea 21 Project to Y.H.S. The nematode strain used in this work was provided by the Caenorhabditis Genetics Center, which is funded by the NIH National Center for Research Resources.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology