Identification of a subnuclear body involved in sequence-specific cytokine RNA processing

Sungwook Lee; Taeyun A. Lee; Eunhye Lee; Sujin Kang; Areum Park; Seung Won Kim; Hyo Jin Park; Je Hyun Yoon; Sang Jun Ha; Taesun Park; Ju Seog Lee; Jae Hee Cheon; Boyoun Park

doi:10.1038/ncomms6791

Identification of a subnuclear body involved in sequence-specific cytokine RNA processing

Sungwook Lee, Taeyun A. Lee, Eunhye Lee, Sujin Kang, Areum Park, Seung Won Kim, Hyo Jin Park, Je Hyun Yoon, Sang Jun Ha, Taesun Park, Ju Seog Lee, Jae Hee Cheon, Boyoun Park

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Processing of interleukin RNAs must be tightly controlled during the immune response. Here we report that a subnuclear body called the interleukin-6 and -10 splicing activating compartment (InSAC) is a nuclear site of cytokine RNA production and stability. Tat-activating regulatory DNA-binding protein-43 (TDP-43) acts as an InSAC scaffold that selectively associates with IL-6 and IL-10 RNAs in a sequence-specific manner. TDP-43 also recruits key spliceosomal components from Cajal bodies. LPS induces posttranslational modifications of TDP-43; in particular, TDP-43 ubiquitination provides a driving force for InSAC formation. As a consequence, in vivo depletion of TDP-43 leads to a dramatic reduction in the RNA processing and the protein levels of IL-6 in serum. Collectively, our findings highlight the importance of TDP-43-mediated InSAC biogenesis in immune regulation.

Original language	English
Article number	5791
Journal	Nature communications
Volume	6
DOIs	https://doi.org/10.1038/ncomms6791
Publication status	Published - 2015 Jan 5

Bibliographical note

Funding Information:
We thank Dr Philip C. Wong (Departments of Pathology, Neuroscience and Neurology, The Johns Hopkins University School of Medicine, Baltimore) for critical reading of the manuscript, and Dr Alxander Hoffmann (University of San Diego, USA) for NF-kB-deficient MEF cells. This study was supported by grants from Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0009203 and 2011-0015372), from the National R&D Program for Cancer Control, Ministry of Health & Welfare, Republic of Korea (1220060), and from the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (HI14C2542). S.L. and J.-H. Y. were supported by the Yonsei University Research Fund of 2014 (2014-12-0135) and by the NIA-IRP, NIH, respectively. S.L., S.K., T.A.L., E.L. and A.P. were supported by Brain Korea (BK21) PLUS Program.

Publisher Copyright:
© 2015 Macmillan Publishers Limited. All rights reserved.

All Science Journal Classification (ASJC) codes

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
General
Physics and Astronomy(all)

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10.1038/ncomms6791

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@article{986858b5f36845deba56a7ce59068401,

title = "Identification of a subnuclear body involved in sequence-specific cytokine RNA processing",

abstract = "Processing of interleukin RNAs must be tightly controlled during the immune response. Here we report that a subnuclear body called the interleukin-6 and -10 splicing activating compartment (InSAC) is a nuclear site of cytokine RNA production and stability. Tat-activating regulatory DNA-binding protein-43 (TDP-43) acts as an InSAC scaffold that selectively associates with IL-6 and IL-10 RNAs in a sequence-specific manner. TDP-43 also recruits key spliceosomal components from Cajal bodies. LPS induces posttranslational modifications of TDP-43; in particular, TDP-43 ubiquitination provides a driving force for InSAC formation. As a consequence, in vivo depletion of TDP-43 leads to a dramatic reduction in the RNA processing and the protein levels of IL-6 in serum. Collectively, our findings highlight the importance of TDP-43-mediated InSAC biogenesis in immune regulation.",

author = "Sungwook Lee and Lee, {Taeyun A.} and Eunhye Lee and Sujin Kang and Areum Park and Kim, {Seung Won} and Park, {Hyo Jin} and Yoon, {Je Hyun} and Ha, {Sang Jun} and Taesun Park and Lee, {Ju Seog} and Cheon, {Jae Hee} and Boyoun Park",

note = "Funding Information: We thank Dr Philip C. Wong (Departments of Pathology, Neuroscience and Neurology, The Johns Hopkins University School of Medicine, Baltimore) for critical reading of the manuscript, and Dr Alxander Hoffmann (University of San Diego, USA) for NF-kB-deficient MEF cells. This study was supported by grants from Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0009203 and 2011-0015372), from the National R&D Program for Cancer Control, Ministry of Health & Welfare, Republic of Korea (1220060), and from the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (HI14C2542). S.L. and J.-H. Y. were supported by the Yonsei University Research Fund of 2014 (2014-12-0135) and by the NIA-IRP, NIH, respectively. S.L., S.K., T.A.L., E.L. and A.P. were supported by Brain Korea (BK21) PLUS Program. Publisher Copyright: {\textcopyright} 2015 Macmillan Publishers Limited. All rights reserved.",

year = "2015",

month = jan,

day = "5",

doi = "10.1038/ncomms6791",

language = "English",

volume = "6",

journal = "Nature Communications",

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T1 - Identification of a subnuclear body involved in sequence-specific cytokine RNA processing

AU - Lee, Sungwook

AU - Lee, Taeyun A.

AU - Lee, Eunhye

AU - Kang, Sujin

AU - Park, Areum

AU - Kim, Seung Won

AU - Park, Hyo Jin

AU - Yoon, Je Hyun

AU - Ha, Sang Jun

AU - Park, Taesun

AU - Lee, Ju Seog

AU - Cheon, Jae Hee

AU - Park, Boyoun

N1 - Funding Information: We thank Dr Philip C. Wong (Departments of Pathology, Neuroscience and Neurology, The Johns Hopkins University School of Medicine, Baltimore) for critical reading of the manuscript, and Dr Alxander Hoffmann (University of San Diego, USA) for NF-kB-deficient MEF cells. This study was supported by grants from Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0009203 and 2011-0015372), from the National R&D Program for Cancer Control, Ministry of Health & Welfare, Republic of Korea (1220060), and from the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (HI14C2542). S.L. and J.-H. Y. were supported by the Yonsei University Research Fund of 2014 (2014-12-0135) and by the NIA-IRP, NIH, respectively. S.L., S.K., T.A.L., E.L. and A.P. were supported by Brain Korea (BK21) PLUS Program. Publisher Copyright: © 2015 Macmillan Publishers Limited. All rights reserved.

PY - 2015/1/5

Y1 - 2015/1/5

N2 - Processing of interleukin RNAs must be tightly controlled during the immune response. Here we report that a subnuclear body called the interleukin-6 and -10 splicing activating compartment (InSAC) is a nuclear site of cytokine RNA production and stability. Tat-activating regulatory DNA-binding protein-43 (TDP-43) acts as an InSAC scaffold that selectively associates with IL-6 and IL-10 RNAs in a sequence-specific manner. TDP-43 also recruits key spliceosomal components from Cajal bodies. LPS induces posttranslational modifications of TDP-43; in particular, TDP-43 ubiquitination provides a driving force for InSAC formation. As a consequence, in vivo depletion of TDP-43 leads to a dramatic reduction in the RNA processing and the protein levels of IL-6 in serum. Collectively, our findings highlight the importance of TDP-43-mediated InSAC biogenesis in immune regulation.

AB - Processing of interleukin RNAs must be tightly controlled during the immune response. Here we report that a subnuclear body called the interleukin-6 and -10 splicing activating compartment (InSAC) is a nuclear site of cytokine RNA production and stability. Tat-activating regulatory DNA-binding protein-43 (TDP-43) acts as an InSAC scaffold that selectively associates with IL-6 and IL-10 RNAs in a sequence-specific manner. TDP-43 also recruits key spliceosomal components from Cajal bodies. LPS induces posttranslational modifications of TDP-43; in particular, TDP-43 ubiquitination provides a driving force for InSAC formation. As a consequence, in vivo depletion of TDP-43 leads to a dramatic reduction in the RNA processing and the protein levels of IL-6 in serum. Collectively, our findings highlight the importance of TDP-43-mediated InSAC biogenesis in immune regulation.

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JO - Nature Communications

JF - Nature Communications

M1 - 5791

ER -

Identification of a subnuclear body involved in sequence-specific cytokine RNA processing

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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