Identification and functional analysis of the fusaricidin biosynthetic gene of Paenibacillus polymyxa E681

Soo Keun Choi, Soo Young Park, Rumi Kim, Choong Hwan Lee, Jihyun F. Kim, Seung Hwan Park

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91 Citations (Scopus)


Fusaricidin, a peptide antibiotic consisting of six amino acids, has been identified as a potential antifungal agent from Paenibacillus polymyxa. Here, we report the complete sequence of the fusaricidin synthetase gene (fusA) identified from the genome sequence of a rhizobacterium, P. polymyxa E681. The gene encodes a polypeptide consisting of six modules in a single open-reading frame. Interestingly, module six of FusA does not contain an epimerization domain, which suggests that the sixth amino acids of the fusaricidin analogs produced by P. polymyxa E681 may exist as an l-form, although all reported fusaricidins contain d-form alanines in their sixth amino acid residues. Alternatively, the sixth adenylation domain of the FusA may directly recognize the d-form alanine. The inactivation of fusA led to the complete loss of antifungal activity against Fusarium oxysporum. LC/MS analysis confirmed the incapability of fusaricidin production in the fusA mutant strain, thus demonstrating that fusA is involved in fusaricidin biosynthesis. Our findings suggested that FusA can produce more than one kind of fusaricidin, as various forms of fusaricidins were identified from P. polymyxa E681.

Original languageEnglish
Pages (from-to)89-95
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number1
Publication statusPublished - 2008 Jan 4

Bibliographical note

Funding Information:
This research was supported by a grant (MG05-0201-6-0) from the 21C Frontier Microbial Genomics and Applications Center Program of the Ministry of Science and Technology, and by a grant (Code 20050401034822) from the BioGreen 21 Program of the Rural Development Administration, Republic of Korea.

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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