Identification and characterization of three telomere repeat-binding factors in rice

Mi Young Byun; Jong Pil Hong; Woo Taek Kim

doi:10.1016/j.bbrc.2008.04.181

Identification and characterization of three telomere repeat-binding factors in rice

Mi Young Byun, Jong Pil Hong, Woo Taek Kim

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Telomeres consist of nucleoprotein complexes that protect chromosome end structures. Here, we describe three OsTRBF genes, encoding telomere repeat-binding factors of the single Myb histone family in rice. The predicted proteins contain a Myb DNA-binding motif and a linker histone H1/H5 domain in the N-terminal and central regions, respectively. The OsTRBF transcripts were constitutively detected in rice plants grown under greenhouse conditions. Gel retardation assays showed that these OsTRBF proteins bind specifically to the plant double-stranded telomeric sequence, TTTAGGG, with markedly different binding affinities as judged by their respective dissociation constants. Yeast two-hybrid and in vitro pull-down assays indicated that both OsTRBF1 and OsTRBF2 interact with one another to form homo- and hetero-complexes, while OsTRBF3 appeared to act as a monomer. Our results suggest that OsTRBFs play combinatory roles in the function and structure of telomeres in rice.

Original language	English
Pages (from-to)	85-90
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	372
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2008.04.181
Publication status	Published - 2008 Jul 18

Bibliographical note

Funding Information:
This work was supported by grants to W.T.K. from the Plant Diversity Research Center (21st Century Frontier Research Program funded by the Ministry of Science and Technology of Korea) and the BioGreen 21 Program (funded by Rural Development Administration of Korea). M.Y.B. was a recipient of Brain Korea 21 graduate student scholarship.

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2008.04.181

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title = "Identification and characterization of three telomere repeat-binding factors in rice",

abstract = "Telomeres consist of nucleoprotein complexes that protect chromosome end structures. Here, we describe three OsTRBF genes, encoding telomere repeat-binding factors of the single Myb histone family in rice. The predicted proteins contain a Myb DNA-binding motif and a linker histone H1/H5 domain in the N-terminal and central regions, respectively. The OsTRBF transcripts were constitutively detected in rice plants grown under greenhouse conditions. Gel retardation assays showed that these OsTRBF proteins bind specifically to the plant double-stranded telomeric sequence, TTTAGGG, with markedly different binding affinities as judged by their respective dissociation constants. Yeast two-hybrid and in vitro pull-down assays indicated that both OsTRBF1 and OsTRBF2 interact with one another to form homo- and hetero-complexes, while OsTRBF3 appeared to act as a monomer. Our results suggest that OsTRBFs play combinatory roles in the function and structure of telomeres in rice.",

author = "Byun, {Mi Young} and Hong, {Jong Pil} and Kim, {Woo Taek}",

note = "Funding Information: This work was supported by grants to W.T.K. from the Plant Diversity Research Center (21st Century Frontier Research Program funded by the Ministry of Science and Technology of Korea) and the BioGreen 21 Program (funded by Rural Development Administration of Korea). M.Y.B. was a recipient of Brain Korea 21 graduate student scholarship. ",

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PY - 2008/7/18

Y1 - 2008/7/18

N2 - Telomeres consist of nucleoprotein complexes that protect chromosome end structures. Here, we describe three OsTRBF genes, encoding telomere repeat-binding factors of the single Myb histone family in rice. The predicted proteins contain a Myb DNA-binding motif and a linker histone H1/H5 domain in the N-terminal and central regions, respectively. The OsTRBF transcripts were constitutively detected in rice plants grown under greenhouse conditions. Gel retardation assays showed that these OsTRBF proteins bind specifically to the plant double-stranded telomeric sequence, TTTAGGG, with markedly different binding affinities as judged by their respective dissociation constants. Yeast two-hybrid and in vitro pull-down assays indicated that both OsTRBF1 and OsTRBF2 interact with one another to form homo- and hetero-complexes, while OsTRBF3 appeared to act as a monomer. Our results suggest that OsTRBFs play combinatory roles in the function and structure of telomeres in rice.

AB - Telomeres consist of nucleoprotein complexes that protect chromosome end structures. Here, we describe three OsTRBF genes, encoding telomere repeat-binding factors of the single Myb histone family in rice. The predicted proteins contain a Myb DNA-binding motif and a linker histone H1/H5 domain in the N-terminal and central regions, respectively. The OsTRBF transcripts were constitutively detected in rice plants grown under greenhouse conditions. Gel retardation assays showed that these OsTRBF proteins bind specifically to the plant double-stranded telomeric sequence, TTTAGGG, with markedly different binding affinities as judged by their respective dissociation constants. Yeast two-hybrid and in vitro pull-down assays indicated that both OsTRBF1 and OsTRBF2 interact with one another to form homo- and hetero-complexes, while OsTRBF3 appeared to act as a monomer. Our results suggest that OsTRBFs play combinatory roles in the function and structure of telomeres in rice.

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Identification and characterization of three telomere repeat-binding factors in rice

Abstract

Bibliographical note

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