Hyper Sensitive Strip Test with Chemi-luminescence Signal Band

Hyung Seok Kim; Jae Chul Pyun

doi:10.1016/j.proche.2009.07.260

Hyper Sensitive Strip Test with Chemi-luminescence Signal Band

Hyung Seok Kim, Jae Chul Pyun

Department of Materials Science and Engineering

Research output: Contribution to journal › Conference article › peer-review

11 Citations (Scopus)

Abstract

A simple and sensitive method using lateral flow immunoassay (LF) using chemiluminescence reaction has been developed for the detection of antigen. In this work, horseradish peroxidase (HRP) samples were used to confirm the validity of this method and human chorionic gonadotropin (hCG) samples were used to evaluate the limit of detection (LOD) of this method. Through HRP test, this method was confirmed to be reliable for immunoassay, and the results of hCG test show that this method was very sensitive compared with conventional strip test using color of gold nanoparticles or latex beads.

Original language	English
Pages (from-to)	1043-1046
Number of pages	4
Journal	Procedia Chemistry
Volume	1
Issue number	1
DOIs	https://doi.org/10.1016/j.proche.2009.07.260
Publication status	Published - 2009 Sept
Event	Eurosensors 23rd Conference - Lausanne, Switzerland Duration: 2009 Sept 6 → 2009 Sept 9

Bibliographical note

Funding Information:
This work was supported by DAPA and ADD, by the Korea Science and Engineering Foundation (KOSEF) grant founded by the Korea Goverment (MOST) (No. R01-2008-000-11767-0), and by the KIST institutional program.

All Science Journal Classification (ASJC) codes

Chemistry(all)
Chemical Engineering(all)

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10.1016/j.proche.2009.07.260

Cite this

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title = "Hyper Sensitive Strip Test with Chemi-luminescence Signal Band",

abstract = "A simple and sensitive method using lateral flow immunoassay (LF) using chemiluminescence reaction has been developed for the detection of antigen. In this work, horseradish peroxidase (HRP) samples were used to confirm the validity of this method and human chorionic gonadotropin (hCG) samples were used to evaluate the limit of detection (LOD) of this method. Through HRP test, this method was confirmed to be reliable for immunoassay, and the results of hCG test show that this method was very sensitive compared with conventional strip test using color of gold nanoparticles or latex beads.",

author = "Kim, {Hyung Seok} and Pyun, {Jae Chul}",

note = "Funding Information: This work was supported by DAPA and ADD, by the Korea Science and Engineering Foundation (KOSEF) grant founded by the Korea Goverment (MOST) (No. R01-2008-000-11767-0), and by the KIST institutional program.; Eurosensors 23rd Conference ; Conference date: 06-09-2009 Through 09-09-2009",

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T1 - Hyper Sensitive Strip Test with Chemi-luminescence Signal Band

AU - Kim, Hyung Seok

AU - Pyun, Jae Chul

N1 - Funding Information: This work was supported by DAPA and ADD, by the Korea Science and Engineering Foundation (KOSEF) grant founded by the Korea Goverment (MOST) (No. R01-2008-000-11767-0), and by the KIST institutional program.

PY - 2009/9

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N2 - A simple and sensitive method using lateral flow immunoassay (LF) using chemiluminescence reaction has been developed for the detection of antigen. In this work, horseradish peroxidase (HRP) samples were used to confirm the validity of this method and human chorionic gonadotropin (hCG) samples were used to evaluate the limit of detection (LOD) of this method. Through HRP test, this method was confirmed to be reliable for immunoassay, and the results of hCG test show that this method was very sensitive compared with conventional strip test using color of gold nanoparticles or latex beads.

AB - A simple and sensitive method using lateral flow immunoassay (LF) using chemiluminescence reaction has been developed for the detection of antigen. In this work, horseradish peroxidase (HRP) samples were used to confirm the validity of this method and human chorionic gonadotropin (hCG) samples were used to evaluate the limit of detection (LOD) of this method. Through HRP test, this method was confirmed to be reliable for immunoassay, and the results of hCG test show that this method was very sensitive compared with conventional strip test using color of gold nanoparticles or latex beads.

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Hyper Sensitive Strip Test with Chemi-luminescence Signal Band

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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